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Elucidation of mechanism of disease using primary failure of tooth eruption specific iPS cells.

Research Project

Project/Area Number 16H07198
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Orthodontics/Pediatric dentistry
Research InstitutionShowa University

Principal Investigator

IZUMIDA ERI  昭和大学, 歯学部, 助教 (70783497)

Project Period (FY) 2016-08-26 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsiPS細胞 / 原発性萌出不全 / PTH1R遺伝子 / ミスセンス変異 / PTH1R
Outline of Final Research Achievements

The purpose of this study was to elucidate the pathogenic mechanism of primary failure of tooth eruption (PFE) using PFE-specific induced pluripotent stem (iPS) cells. PFE-specific iPS cells were prepared from hematopoetic precursor cells isolated from blood of a PFE patient by introducing Yamanaka factors (OCT3/4, SOX2, and KLF4) using a Sendai virus vector. Osteoblast-like cells were obtained after cultivation of PFE-specific and control iPS cells in the osteoblastic differentiation medium. Expression of osteoblast marker genes in as well as mineralization by osteoblast-like cells derived from PFE-specific iPS cells was comparable to those obtained in cells derived from control iPS cells. These results indicate that the mutation in PTH1R gene found in the PFE patient does not affect osteoblast differentiation.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • Research Products

    (1 results)

All 2016

All Presentation (1 results)

  • [Presentation] 疾患特異的iPS細胞を用いた原発性萌出不全発症機序の解析2016

    • Author(s)
      泉田恵理
    • Organizer
      第75回日本矯正歯科学会大会
    • Place of Presentation
      アスティとくしま
    • Year and Date
      2016-11-07
    • Related Report
      2016 Annual Research Report

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Published: 2016-09-02   Modified: 2019-03-29  

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