| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The aim of this study is to profile the gene expression during osteogenic differentiation in stem cells derived from transgenic mice with Cre/LoxP system. We could confirm the expression of EGFP in osteocyte-like cells, which exhibited dendrite-like structures in OBM for 2 weeks. Differentiated BMSCs were divided into EGFP positive and negative cells by Flow cytometry. DNA microarray analysis demonstrated differences in the expression levels of some genes and some relevance (including Wnt signaling) in upregulated genes in EGFP positive cells. Cryab (crystalline-αB) and some osteogenic marker genes (osteocalcin, bone sialoprotein, Dmp1) were expressed at significantly higher levels in EGFP positive cells than EGFP negative cells. These results indicated that CRYAB express at around the same time as DMP1 expression during osteogenic differentiation and may contribute to the differentiation into osteocyte and the formation of mineralized tissues.
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