| Project/Area Number |
16H07243
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Aquatic life science
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| Research Institution | Toyo University |
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Principal Investigator |
INOKUCHI Mayu 東洋大学, 生命科学部, 助教 (90778549)
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 軟骨魚類 / 浸透圧調節 / 塩類細胞 / オオメジロザメ / プロラクチン |
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| Outline of Final Research Achievements |
To clarify the role of elasmobranch ionocyte in osmoregulation, we used euryhaline bull shark as experimental fish. The transcriptome analyses were performed on bull shark gills, and we focused on CLC2, ECaC, and PMCA. ECaC expression was increased after FW acclimation. The distribution of CLC2 and PMCA signals were similar to that of ionocytes. These findings indicate that CLC2, ECaC and PMCA are involved in ionoregulation in the gills. Additionally, we examine the role of prolactin in bull shark. The results showed that prolactin and its receptor is highly expressed in pituitary.
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