Isolation/identification of novel darkness inducible cell death factors by forward genetics using autophagy-defective plants
| Project/Area Number |
16H07255
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Meiji University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | タンパク質分解 / 老化 / 細胞死 / オートファジー / オルガネラ / サプレッサースクリーニング |
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| Outline of Final Research Achievements |
Autophagy is the major system responsible for the degradation of organelles and cytosolic macromolecules in the vacuole. So far, I have found that autophagy defective mutants (atg mutants) show dark-induced senescence phenotype. However, the reason why defect of autophagy accelerates the senescence phenotype remains to be elucidated.
In this research, I screened suppressors which do not show the accelerated dark-induced senescence phenotype, and isolated 6 independent suppressors. Then, by whole genome sequencings, I identified the putative responsible genes for the suppressors. I also tried to elucidate the physiological functions of plant autophagy under the darkness and further establish research bases for elucidation of molecular mechanisms of dark-induced senescence processes.
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Report
(3 results)
Research Products
(14 results)
