The study of the synaptonemal complex component SYCP3 in meiotic recombination

• Project/Area Number: 16H07295
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Genome biology
• Research Institution: Waseda University
• Principal Investigator: Wataru Kobayashi 早稲田大学, 理工学術院, 助教 (20778063)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 相同組換え / シナプトネマ複合体 / SYCP3 / RAD51 / DMC1 / 相同的対合 / 減数分裂期組換え / 相同的対合反応

Outline of Final Research Achievements

The purpose of this study is to elucidate the molecular mechanism of meiotic recombination by the synpatonemal complex component SYCP3. The synaptonemal complex is essential for the progression of meiotic recombination. However, the functions of SYCP3 in meiotic recombination remain elusive. We performed the biochemical and cell biology analyses to elucidate the mechanism by which SYCP3 regulates RAD51- and DMC1- homologous pairing. We found that SYCP3 significantly suppresses the RAD51-mediated homologous pairing, but not DMC1-mediated homologous pairing. In addition, we found that SYCP3 specifically binds to RAD51 and suppresses RAD51-mediated homologous pairing by competing with HOP2-MND1 complex.

Research Products

• [Journal Article] Replication stress induces accumulation of FANCD2 at central region of large fragile genes. (2018)
  • Author(s): Okamoto Y, Iwasaki WM, Kugou K, Takahashi KK, Oda A, Sato K, Kobayashi W, Kawai H, Sakasai R, Takaori-Kondo A, Yamamoto T, Kanemaki MT, Taoka M, Isobe T, Kurumizaka H, Innan H, Ohta K, Ishiai M, Takata M.
  • Journal Title: Nucleic Acids Res. Volume: 46(6) Issue: 6 Pages: 2932-2944
  • DOI: 10.1093/nar/gky058
  • NAID: 120006488604
  • Peer Reviewed / Open Access
• [Journal Article] SYCP3 regulates strand invasion activities of RAD51 and DMC1 (2017)
  • Author(s): Kobayashi Wataru、Hosoya Noriko、Machida Shinichi、Miyagawa Kiyoshi、Kurumizaka Hitoshi
  • Journal Title: Genes to Cells Volume: 22 Issue: 9 Pages: 799-809
  • DOI: 10.1111/gtc.12513
  • Peer Reviewed / Open Access
• [Journal Article] RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination (2017)
  • Author(s): Inano Shojiro、Sato Koichi、Katsuki Yoko、Kobayashi Wataru、Tanaka Hiroki、Nakajima Kazuhiro、Nakada Shinichiro、Miyoshi Hiroyuki、Knies Kerstin、Takaori-Kondo Akifumi、Schindler Detlev、Ishiai Masamichi、Kurumizaka Hitoshi、Takata Minoru
  • Journal Title: Molecular Cell Volume: 66 Issue: 5 Pages: 622-634
  • DOI: 10.1016/j.molcel.2017.04.022
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Xeroderma pigmentosum group C protein interacts with histones: regulation by acetylated states of histone H3. (2017)
  • Author(s): Kakumu E
  • Journal Title: Genes Cells. Volume: 3 Issue: 3 Pages: 310-327
  • DOI: 10.1111/gtc.12479
  • Peer Reviewed / Open Access
• [Journal Article] FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5'-DNA end. (2016)
  • Author(s): Sato K, Shimomuki M, Katsuki Y, Takahashi D, Kobayashi W, Ishiai M, Miyoshi H, Takata M, Kurumizaka H.
  • Journal Title: Nucleic Acids Res Volume: 44 Issue: 22 Pages: 10758-10771
  • DOI: 10.1093/nar/gkw876
  • NAID: 120006318854
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Chromatin architecture may dictate the target site for DMC1, but not for RAD51, during homologous pairing (2016)
  • Author(s): Kobayashi W., Takaku M., Machida S., Tachiwana H., Maehara K., Ohkawa Y. and Kurumizaka H
  • Journal Title: Scientific reports Volume: 6 Issue: 1 Pages: 24428-24428
  • DOI: 10.1038/srep24228
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Functional analysis of the synaptonemal complex component SYCP3 (2018)
  • Author(s): Kobayashi W, Kurumizaka H
  • Organizer: INDO-JAPAN Conference: Epigenetics, Human Microbiomes and Disease
  • Int'l Joint Research
• [Presentation] Structural and biochemical analysis of overlapping dinucleosome containing histone variants and histone modifications (2017)
  • Author(s): Kobayashi W, Aihara M, Kato D, Koyama M, Saikusa K, Akashi S, Inoue R, Sugiyama M, Kurumizaka H
  • Organizer: EMBO Conference The Nucleosome: From Atoms to Genomes
  • Int'l Joint Research
• [Presentation] ヌクレオソーム上におけるRAD51の相同鎖検索機構の解析 (2017)
  • Author(s): 小林航、石井初芽、胡桃坂仁志
  • Organizer: 第24回 DNA複製・組換え・修復ワークショップ
• [Presentation] シナプトネマ複合体構成因子SYCP3はRAD51およびDMC1依存的な相同組換え反応を制御する (2016)
  • Author(s): 小林航、細谷紀子、寺本睦美、町田晋一、宮川清、胡桃坂仁志
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、 横浜
  • Year and Date: 2016-11-30
• [Presentation] The synaptonemal complex component SYCP3 regulates meiotic recombination (2016)
  • Author(s): Wataru Kobayashi, Noriko Hososya, Mutsumi Teramoto, Shinichi Machida, Kiyoshi Miyagawa, Hitoshi Kurumizaka
  • Organizer: 10th 3R International Symposium
  • Place of Presentation: Matue city, Shimane, JAPAN
  • Year and Date: 2016-11-13
  • Int'l Joint Research
• [Remarks] 胡桃坂研究室
  • URL: http://www.eb.waseda.ac.jp/kurumizaka/