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The study of the synaptonemal complex component SYCP3 in meiotic recombination

Research Project

Project/Area Number 16H07295
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Genome biology
Research InstitutionWaseda University

Principal Investigator

Wataru Kobayashi  早稲田大学, 理工学術院, 助教 (20778063)

Project Period (FY) 2016-08-26 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords相同組換え / シナプトネマ複合体 / SYCP3 / RAD51 / DMC1 / 相同的対合 / 減数分裂期組換え / 相同的対合反応
Outline of Final Research Achievements

The purpose of this study is to elucidate the molecular mechanism of meiotic recombination by the synpatonemal complex component SYCP3. The synaptonemal complex is essential for the progression of meiotic recombination. However, the functions of SYCP3 in meiotic recombination remain elusive. We performed the biochemical and cell biology analyses to elucidate the mechanism by which SYCP3 regulates RAD51- and DMC1- homologous pairing. We found that SYCP3 significantly suppresses the RAD51-mediated homologous pairing, but not DMC1-mediated homologous pairing. In addition, we found that SYCP3 specifically binds to RAD51 and suppresses RAD51-mediated homologous pairing by competing with HOP2-MND1 complex.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • Research Products

    (12 results)

All 2018 2017 2016 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (5 results) (of which Int'l Joint Research: 3 results) Remarks (1 results)

  • [Journal Article] Replication stress induces accumulation of FANCD2 at central region of large fragile genes.2018

    • Author(s)
      Okamoto Y, Iwasaki WM, Kugou K, Takahashi KK, Oda A, Sato K, Kobayashi W, Kawai H, Sakasai R, Takaori-Kondo A, Yamamoto T, Kanemaki MT, Taoka M, Isobe T, Kurumizaka H, Innan H, Ohta K, Ishiai M, Takata M.
    • Journal Title

      Nucleic Acids Res.

      Volume: 46(6) Issue: 6 Pages: 2932-2944

    • DOI

      10.1093/nar/gky058

    • NAID

      120006488604

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] SYCP3 regulates strand invasion activities of RAD51 and DMC12017

    • Author(s)
      Kobayashi Wataru、Hosoya Noriko、Machida Shinichi、Miyagawa Kiyoshi、Kurumizaka Hitoshi
    • Journal Title

      Genes to Cells

      Volume: 22 Issue: 9 Pages: 799-809

    • DOI

      10.1111/gtc.12513

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination2017

    • Author(s)
      Inano Shojiro、Sato Koichi、Katsuki Yoko、Kobayashi Wataru、Tanaka Hiroki、Nakajima Kazuhiro、Nakada Shinichiro、Miyoshi Hiroyuki、Knies Kerstin、Takaori-Kondo Akifumi、Schindler Detlev、Ishiai Masamichi、Kurumizaka Hitoshi、Takata Minoru
    • Journal Title

      Molecular Cell

      Volume: 66 Issue: 5 Pages: 622-634

    • DOI

      10.1016/j.molcel.2017.04.022

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Xeroderma pigmentosum group C protein interacts with histones: regulation by acetylated states of histone H3.2017

    • Author(s)
      Kakumu E
    • Journal Title

      Genes Cells.

      Volume: 3 Issue: 3 Pages: 310-327

    • DOI

      10.1111/gtc.12479

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5'-DNA end.2016

    • Author(s)
      Sato K, Shimomuki M, Katsuki Y, Takahashi D, Kobayashi W, Ishiai M, Miyoshi H, Takata M, Kurumizaka H.
    • Journal Title

      Nucleic Acids Res

      Volume: 44 Issue: 22 Pages: 10758-10771

    • DOI

      10.1093/nar/gkw876

    • NAID

      120006318854

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chromatin architecture may dictate the target site for DMC1, but not for RAD51, during homologous pairing2016

    • Author(s)
      Kobayashi W., Takaku M., Machida S., Tachiwana H., Maehara K., Ohkawa Y. and Kurumizaka H
    • Journal Title

      Scientific reports

      Volume: 6 Issue: 1 Pages: 24428-24428

    • DOI

      10.1038/srep24228

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Functional analysis of the synaptonemal complex component SYCP32018

    • Author(s)
      Kobayashi W, Kurumizaka H
    • Organizer
      INDO-JAPAN Conference: Epigenetics, Human Microbiomes and Disease
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural and biochemical analysis of overlapping dinucleosome containing histone variants and histone modifications2017

    • Author(s)
      Kobayashi W, Aihara M, Kato D, Koyama M, Saikusa K, Akashi S, Inoue R, Sugiyama M, Kurumizaka H
    • Organizer
      EMBO Conference The Nucleosome: From Atoms to Genomes
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヌクレオソーム上におけるRAD51の相同鎖検索機構の解析2017

    • Author(s)
      小林航、石井初芽、胡桃坂仁志
    • Organizer
      第24回 DNA複製・組換え・修復ワークショップ
    • Related Report
      2017 Annual Research Report
  • [Presentation] シナプトネマ複合体構成因子SYCP3はRAD51およびDMC1依存的な相同組換え反応を制御する2016

    • Author(s)
      小林航、細谷紀子、寺本睦美、町田晋一、宮川清、胡桃坂仁志
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、 横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] The synaptonemal complex component SYCP3 regulates meiotic recombination2016

    • Author(s)
      Wataru Kobayashi, Noriko Hososya, Mutsumi Teramoto, Shinichi Machida, Kiyoshi Miyagawa, Hitoshi Kurumizaka
    • Organizer
      10th 3R International Symposium
    • Place of Presentation
      Matue city, Shimane, JAPAN
    • Year and Date
      2016-11-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 胡桃坂研究室

    • URL

      http://www.eb.waseda.ac.jp/kurumizaka/

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-09-02   Modified: 2019-03-29  

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