| Project/Area Number |
16H07312
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokai Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Chlamydia / Parachlamydia / Chlamydia trachomatis / Brevibacillus / 細菌 / 遺伝子 / 発現制御 / 性器クラミジア / パラクラミジア / ブレビバチルス発現システム / クラミジア |
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| Outline of Final Research Achievements |
Usual chlamydial protein expression system is expressing in E. coli, but it is not very efficiently. So, I challenged to establishing of an expression system by using Brevibacillus Expression System II.
Parachlamydia acanthamoeba was used in the experiment, it is easy to handling and closely related species for Chlamydia trachomatis that is the serious issue of a cause of sexually transmitted disease. Consequently, chlamydial proteins was not expressed by using this system in this time. It may be necessary to compare the difference of characteristics of the gene sequence encoding amino acids between Chlamydia, E. coli and Brevibacillus, and so on, but this research is thought to be a help for chlamydial research in the future.
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