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Attempts at Elucidation of anaerobic bulking phenomenon for wastewater treatment and development of diagnostic technology of bulking-causative microorganisms

Research Project

Project/Area Number 16H07403
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Civil and environmental engineering
Research InstitutionMiyakonojo National College of Technology (2017)
Kitakyushu National College of Technology (2016)

Principal Investigator

Kuroda Kyohei  都城工業高等専門学校, 物質工学科, 助教 (50783213)

Research Collaborator YAMADA Masayoshi  鹿児島工業高等専門学校, 都市環境デザイン工学科, 准教授 (80469593)
NARIHIRO Takashi  産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (20421844)
NOBU K. Masaru  産業技術総合研究所, 生物プロセス研究部門, 研究員 (40805644)
Project Period (FY) 2016-08-26 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords嫌気性廃水処理 / グラニュール汚泥 / バルキング現象 / 環境ゲノム解析 / FISH法 / メタン発酵促進 / 16S rRNA遺伝子解析 / メタゲノム解析 / 嫌気性廃水処理法 / 芳香族化合物含有廃水 / バルキング / オミクス / メタボローム解析
Outline of Final Research Achievements

This study focused on sludge bulking occurred in upflow anaerobic sludge blanket (UASB) reactor treating phenol wastewater. In this study, to elucidate causative factors of the bulking, we performed metagenomic analysis of settling and floated granules. In addition, we attempted to develop methanogeneis facilitating technology using green tuff, which is porous and contains several conductive and essential minerals. Metagenomic analysis indicated that abundance of phenol, butyrate, and benzoate-degrading organisms and methanogens were suddenly decreased after bulking occurrence. Besides, abundance of predominant uncultured archaea in setting sludge were also decreased. By functional analysis, this archaea might have parasitic lifestyle in the UASB reactor. Using green tuff for development of methanogenesis facilitating technology, we confirmed that methane gas production rate was increased with the green tuff, suggesting that the material can be used to support methanogenesis.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • Research Products

    (7 results)

All 2018 2017 2016

All Presentation (7 results) (of which Int'l Joint Research: 3 results)

  • [Presentation] 天然鉱物を用いたメタン発酵促進技術の開発2018

    • Author(s)
      黒田恭平,延優,幡本将史,成廣隆,山口隆司,山内正仁,山田真義
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] フェノール模擬廃水を処理するUASB反応槽保持汚泥の肥大化の原因探索2017

    • Author(s)
      黒田恭平,高見誠也,平片悠河,幡本将史,山口隆司,山内正仁,成廣隆,山田真義
    • Organizer
      第51回日本水環境学会年会
    • Place of Presentation
      熊本大学 黒髪キャンパス(熊本県熊本市)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Exploration of uncultured bacterial phyla in biological wastewater treatment systems2017

    • Author(s)
      Kyohei Kuroda, Masashi Hatamoto, Masayoshi Yamada, Masahito Yamauchi, Takashi Yamaguchi
    • Organizer
      The 2nd International Symposium on Expertise of Engineering Design (2nd ISEED)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Detection of bulking-causative microorganisms in an upflow anaerobic sludge blanket reactor using molecular techniques2017

    • Author(s)
      Kyohei Kuroda, Yu Ikedo, Tomoka Arata, Nana Ohmine, Hazuki Kurashita, Manami Kotsusa, Yuga Hirakata, Masashi Hatamoto, Masayoshi Yamada, Masahito Yamauchi, Takashi Yamaguchi
    • Organizer
      The 6th International GIGAKU Conference (IGCN 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生物学的廃水処理汚泥を対象とした高解像度16S rRNA遺伝子解析2017

    • Author(s)
      黒田恭平,延優,成廣隆,山口隆司
    • Organizer
      第20回日本水環境学会シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] エネルギー・食料生産技術の高効率化に向けた微生物学・分子生物学的解析手法の適用2017

    • Author(s)
      黒田恭平
    • Organizer
      宮崎大学 産学・地域連携センター 第24回技術・研究発表交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Microbial community composition of floated and overgrown granules in an upflow anaerobic sludge blanket reactor2016

    • Author(s)
      Kyohei Kuroda, Seiya Takami, Yuga Hirakata, Masashi Hatamoto, Takashi Yamaguchi, Masahito Yamauchi, Takashi Narihiro, Masayoshi Yamada
    • Organizer
      11th International Forum on Ecotechnology in Penang (IFE11)
    • Place of Presentation
      Toyohashi University of Technology - Universiti Sains Malaysia
    • Year and Date
      2016-12-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2016-09-02   Modified: 2019-03-29  

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