Analyses of regulation mechanism of DNA replication and its evolutionary significance in cyanobacteria and chloroplast
Project/Area Number |
16H07418
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Genome biology
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Research Institution | National Institute of Genetics |
Principal Investigator |
Ohbayashi Ryudo 国立遺伝学研究所, 細胞遺伝研究系, 博士研究員 (90778333)
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Project Period (FY) |
2016-08-26 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | DnaA / DNA複製 / ゲノム倍数性 / シアノバクテリア / 葉緑体 / 共生進化 / 藍藻 / DNAコーミング / 進化 / ゲノム / 微生物 / 遺伝学 |
Outline of Final Research Achievements |
Chromosome ploidy has diversified during evolution in all of the three domains of life, bacteria, archaea, and eukaryotes. Cyanobacteria (and chloroplast) maintain multiple copies of chromosomes (genomes). Although a correlation between ploidy level and cell size has been observed in bacteria and eukaryotes, it is poorly understood how replication of multi-copy chromosomes is regulated and how ploidy level is adjusted to cell size. Here we show that only one (or a few) copy of multiple chromosomes is replicated at once in the cyanobacteria and that this restriction depends on regulation of DnaA activity. Moreover, when cell growth rate was increased or decreased at a constant temperature, DnaA level, DnaA activity, and the number of replicating chromosomes also increased or decreased, resulting in nearly constant chromosome copy number per unit cell volume.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] Development of a double nuclear gene-targeting method by two-step transformation based on a newly established chloramphenicol-selection system in the red alga Cyanidioschyzon merolae2017
Author(s)
Fujiwara, T., Ohnuma, M., Kuroiwa, T., Ohbayashi, R., Hirooka, S.and Miyagishima, S.
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Journal Title
Front. Plant Sci.
Volume: 8
Pages: 343-343
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Acidophilic green algal genome provides insights into adaptation to an acidic environment2017
Author(s)
Hirooka, S., Hirose, Y., Kanesaki, Y., Higuchi, S., Fujiwara, T., Onuma, R., Era, A., Ohbayashi, R., Uzuka, A., Nozaki, H., Yoshikawa, H., and Miyagishima, S.
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Journal Title
Proc. Natl. Acad. Sci. USA.
Volume: 114
Issue: 39
DOI
Related Report
Peer Reviewed / Open Access
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