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Evaluating the Influence of Viruses on the Deep Biosphere Carbon Cycle

Research Project

Project/Area Number 16H07489
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Environmental dynamic analysis
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

PAN Donald  国立研究開発法人海洋研究開発機構, 深海・地殻内生物圏研究分野, ポストドクトラル研究員 (90784851)

Project Period (FY) 2016-08-26 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordssubseafloor / virus / sediment / subsurface / biogeochemistry / carbon
Outline of Final Research Achievements

Subseafloor sediment (200-1200m below seafloor) was collected during IODP Expedition 370 in the Nankai Trough. The sediment biomass was extremely low. Using traditional methods of virus extraction from sediment, it was not possible to find viruses. I developed a new method of virus extraction from sediment that improved extraction by more than 100x. This suggests that there are many more viruses in subseafloor sediment than previously known. In order to obtain higher biomass samples for analysis, subseafloor sediment from another site (offshore Cape Erimo) were obtained by drilling to 100mbsf during Chikyu Expedition 910. Cell extraction for TEM analysis and viral DNA extraction is in progress. An alternative approach was also taken using viruses cultivated from a methanogenic bioreactor which had been initially seeded with subseafloor sediment from offshore Shimokita during IODP Expedition 337. Viral DNA was extracted and sequenced. Bioinformatic analysis is in progress.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • Research Products

    (8 results)

All 2018 2017

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 2 results,  Open Access: 3 results) Presentation (5 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Journal Article] Exploring the Role of Relational Practices in Water Governance Using a Game-Based Approach2018

    • Author(s)
      Magnuszewski Piotr、Krolikowska Karolina、Koch Anna、Pajak Michal、Allen Craig、Chraibi Victoria、Giri Anil、Haak Danielle、Hart Noelle、Hellman Michelle、Pan Donald、Rossman Nathan、Sendzimir Jan、Sliwinski Maggi、Stefanska Joanna、Taillieu Tharsi、Weide Denise、Zlatar Ilonka
    • Journal Title

      Water

      Volume: 10 Issue: 3 Pages: 346-346

    • DOI

      10.3390/w10030346

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Abundance and Distribution of Microbial Cells and Viruses in an Alluvial Aquifer2017

    • Author(s)
      Pan Donald、Nolan Jason、Williams Kenneth H.、Robbins Mark J.、Weber Karrie A.
    • Journal Title

      Frontiers in Microbiology

      Volume: 8

    • DOI

      10.3389/fmicb.2017.01199

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] International Ocean Discovery Program Expedition 370 Preliminary Report: Temperature Limit of the Deep Biosphere off Muroto2017

    • Author(s)
      Verena B. Heuer, Fumio Inagaki, Yuki Morono, Yusuke Kubo, Lena Maeda, and Expedition 370 Scientists
    • Journal Title

      International Ocean Discovery Program

      Volume: ** Pages: 1-29

    • DOI

      10.14379/iodp.pr.370.2017

    • Related Report
      2016 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Presentation] Preliminary results from IODP Expedition 370 (Temperature Limit of the Deep Biosphere off Muroto, T-LIMIT)2017

    • Author(s)
      V.B. HEUER, F. INAGAKI, Y. MORONO, Y. KUBO, S. HENKEL, F. SCHUBOTZ, B. VIEHWEGER, AND THE IODP EXPEDITION 370 SCIENTISTS
    • Organizer
      IODP/ICDP Kolloquium 2017
    • Place of Presentation
      Braunschweig, Germany
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] IODP 室戸沖限界生命圏掘削調査(T-Limit)の概要について2017

    • Author(s)
      稲垣史生, Verena B. Heuer, 諸野祐樹, 久保雄介, IODP Expedition 370 Scientists
    • Organizer
      ブルーアースシンポジウム2017
    • Place of Presentation
      日本大学(東京部千代田区)
    • Year and Date
      2017-03-02
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 「ちきゅう」による IODP 室戸沖限界生命圏掘削調査(T-Limit)のオペレーション概要2017

    • Author(s)
      久保雄介・稲垣史生・諸野祐樹,Verena Heuer,IODP Expedition 370 Scientists
    • Organizer
      ブルーアースシンポジウム2017
    • Place of Presentation
      日本大学(東京部千代田区)
    • Year and Date
      2017-03-02
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Improved virus extraction and enumeration from deep subsurface sediment2017

    • Author(s)
      Donald Pan
    • Organizer
      International Society for Subsurface Microbiology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expanding the Frontiers of the Virosphere: Viruses in the Subsurface2017

    • Author(s)
      Donald Pan
    • Organizer
      Japan Geoscience Union - American Geophysical Union Join Meeting 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2016-09-02   Modified: 2019-12-27  

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