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Study for the realization of a new detection method for irradiated foods by measuring 5,6-Dihydrothymidine

Research Project

Project/Area Number 16H07502
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Hygiene and public health
Research InstitutionOsaka Institute of Public Health

Principal Investigator

Takuya Fujiwara  地方独立行政法人 大阪健康安全基盤研究所, 衛生化学部, 研究員 (70783819)

Project Period (FY) 2016-08-26 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords食品 / 食品照射 / 放射線 / 電子線 / 質量分析 / LC-MS/MS / 衛生 / 分析科学
Outline of Final Research Achievements

The method for determination of irradiation histories of foods by measuring the ratio of sum of 5R- and 5S-5,6-dihidrothymidine to thymidine (total-DHdThd/dThd) in DNA as a marker was applicable to electron beam (EB) irradiated foods as γ-irradiated foods. The production efficiency of DHdThd to irradiation dose was similar between the EB and the γ-irradiated samples. The total-DHdThd/dThd in irradiated samples was stable during the storage for 4 months at -20℃ and process of heating as cook. Thus, the total-DHdThd/dThd would be a rugged marker for determination for irradiation history of foods. The isomer ratio of 5R- and 5S-DHdThd may be sensitive to the temperature of sample irradiation, although further studies would be required.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • Research Products

    (5 results)

All 2018 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Rapid and Reliable Method for Determining Irradiation Histories of Ground Beef and Prawns by Measuring 5,6-Dihydrothymidine2017

    • Author(s)
      Naoki Fukui, Satoshi Takatori, Yoko Kitagawa, Takuya Fujiwara, Etsuko Ishikawa, Takatomo Fujiyama, Keiji Kajimura, Masakazu Furuta, Hirotaka Obana
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 65 Issue: 42 Pages: 9342-9352

    • DOI

      10.1021/acs.jafc.7b03266

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] 放射線損傷ヌクレオシドである5,6-ジヒドロチミジンを指標とした照射食品検知法の開発(電子線照射食品への適用)2018

    • Author(s)
      藤原拓也、福井直樹、高取聡、石川悦子、藤山貴友、梶村計志、古田雅一
    • Organizer
      第53回日本食品照射研究協議会研究発表会(東京都江東区)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 放射線損傷ヌクレオシドである5,6-ジヒドロチミジンを指標とした照射食品検知法の開発2017

    • Author(s)
      福井直樹、藤原拓也、北川陽子、高取聡、石川悦子、藤山貴友、梶村計志、古田雅一、尾花裕孝
    • Organizer
      第54回アイソトープ・放射線研究発表会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2017-07-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] 放射線損傷ヌクレオシドである5,6-ジヒドロチミジンを指標とした照射食品検知法の電子線照射食品への適用性2017

    • Author(s)
      藤原拓也、福井直樹、高取聡、石川悦子、藤山貴友、梶村計志、古田雅一
    • Organizer
      第113回日本食品衛生学会学術講演会(東京都江戸川区)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 放射線損傷ヌクレオシドである5,6-ジヒドロチミジンを指標とした照射食品検知法の開発2016

    • Author(s)
      福井直樹、高取聡、北川陽子、藤原拓也、起橋雅浩、 石川悦子、藤山貴友、梶村計志、古田雅一、尾花裕孝
    • Organizer
      日本食品照射研究協議会第52回大会
    • Place of Presentation
      東京都立産業技術研究センター(東京都江東区)
    • Year and Date
      2016-12-07
    • Related Report
      2016 Annual Research Report

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Published: 2016-09-02   Modified: 2019-03-29  

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