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Streptomyces属放線菌におけるComXフェロモン型分子の探索

Research Project

Project/Area Number 16J01117
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Natural medicines
Research InstitutionThe University of Tokyo

Principal Investigator

星野 翔太郎  東京大学, 薬学系研究科, 特別研究員(DC1)

Project Period (FY) 2016-04-22 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2018: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2017: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2016: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords代謝解析 / 遺伝子破壊株 / 形態比較 / Quorum sensing / 二糖類 / 遺伝子破壊 / 相同組み換え法 / 接合伝達 / 基質認識 / プレニル基転移酵素 / Streptomyces属 / 放線菌 / クオラムセンシング / ゲノムマイニング / ペプチドフェロモン / 翻訳後修飾 / イソプレニル化
Outline of Annual Research Achievements

本年度は、Streptomyces bottropensis野生株及びsboQ遺伝子の破壊株(昨年度作成)を用いることで、本遺伝子によって生合成される代謝物の同定、及び本遺伝子の放線菌Streptomyces bottropensis株に対する生物的意義の解明を目指した。
まず、sboQ遺伝子により生合成される代謝物の同定を目的として複数種類の液体培地にて野生株及び遺伝子破壊株の培養及び抽出を行い、HPLC及びLC-MSによる分析を行ったが、これまでの所野生株・破壊株間で目立った代謝プロファイル変化を確認することは出来なかった。
続いてsboQ遺伝子の生物的意義を明らかとする目的として、炭素源を中心として100種類以上の成分の異なる寒天培地を作成し、野生株及び破壊株の各寒天培地上における表現型の違いを観察した。その結果、破壊株は野生株と比較して高濃度の二糖類(LactoseやSucroseなど)を含有する寒天培地上では生育が著しく悪化することが見いだされた。一方、高濃度のSorbitolを含有する培地では両者の生育に差が見られなかったとから(即ち単純な浸透圧による影響ではない)、sboQ遺伝子がQuorum sensingを介して糖代謝あるいは糖輸送を制御している事が強く示唆された。
遺伝子相補実験によって上記現象に対するsboQ遺伝子の関与が確認されれば、放線菌におけるpeptide分子を介した未知のquorum sensing系の存在に関する糸口を与えると共に、それらを利用した抗生物質生産制御など幅広い応用が期待され、その意義は極めて大きいものと言える。

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (29 results)

All 2019 2018 2017 2016

All Journal Article (17 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 17 results,  Open Access: 6 results,  Acknowledgement Compliant: 3 results) Presentation (12 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Lignans with melanogenesis effects from Premna serratifolia wood2019

    • Author(s)
      S. Woo, S. Hoshino, C.P. Wong, N.N. Win, M.D. Awoufack, Prema, H. Ngwe, H. Zhang, F. Hayashi, I. Abe, H. Morita
    • Journal Title

      Fitoterapia

      Volume: 133 Pages: 35-42

    • DOI

      10.1016/j.fitote.2018.12.008

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A New Tetrahydrofuran Lignan from Premna serratifolia Wood2019

    • Author(s)
      Woo So-Yeun、Wong Chin Piow、Win Nwet Nwet、Hoshino Shotaro、Prema、Ngwe Hla、Abe Ikuro、Morita Hiroyuki
    • Journal Title

      Natural Product Communications

      Volume: 14 Issue: 1 Pages: 113-116

    • DOI

      10.1177/1934578x1901400130

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Complete biosynthetic pathways of ascofuranone and ascochlorin in Acremonium egyptiacum2019

    • Author(s)
      Araki Yasuko、Awakawa Takayoshi、Matsuzaki Motomichi、Cho Rihe、Matsuda Yudai、Hoshino Shotaro、Shinohara Yasutomo、Yamamoto Masaichi、Kido Yasutoshi、Inaoka Daniel Ken、Nagamune Kisaburo、Ito Kotaro、Abe Ikuro、Kita Kiyoshi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 未定 Issue: 17 Pages: 8269-8274

    • DOI

      10.1073/pnas.1819254116

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Reprogramming of the antimycin NRPS-PKS assembly lines inspired by gene evolution2018

    • Author(s)
      Awakawa Takayoshi、Fujioka Takuma、Zhang Lihan、Hoshino Shotaro、Hu Zhijuan、Hashimoto Junko、Kozone Ikuko、Ikeda Haruo、Shin-Ya Kazuo、Liu Wen、Abe Ikuro
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 3534-3534

    • DOI

      10.1038/s41467-018-05877-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Crystalline Sponge Method Enabled the Investigation of a Prenyltransferase-terpene Synthase Chimeric Enzyme, Whose Product Exhibits Broadened NMR Signals2018

    • Author(s)
      Mitsuhashi Takaaki、Kikuchi Takashi、Hoshino Shotaro、Ozeki Masahiro、Awakawa Takayoshi、Shi She-Po、Fujita Makoto、Abe Ikuro
    • Journal Title

      Organic Letters

      Volume: 20 Issue: 18 Pages: 5606-5609

    • DOI

      10.1021/acs.orglett.8b02284

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Catenulobactins A and B, Heterocyclic Peptides from Culturing Catenuloplanes sp. with a Mycolic Acid-Containing Bacterium2018

    • Author(s)
      Hoshino Shotaro、Ozeki Masahiro、Awakawa Takayoshi、Morita Hiroyuki、Onaka Hiroyasu、Abe Ikuro
    • Journal Title

      Journal of Natural Products

      Volume: 81 Issue: 9 Pages: 2106-2110

    • DOI

      10.1021/acs.jnatprod.8b00261

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hinduchelins A-D, Noncytotoxic Catechol Derivatives from <i>Streptoalloteichus hindustanus</i>2018

    • Author(s)
      He Fei、Nakamura Hitomi、Hoshino Shotaro、Fong Chin Joyce Seow、Yang Liang、Zhang Huiping、Hayashi Fumiaki、Abe Ikuro
    • Journal Title

      Journal of Natural Products

      Volume: 81 Issue: 6 Pages: 1493-1496

    • DOI

      10.1021/acs.jnatprod.8b00049

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Activation of silent biosynthetic pathways and discovery of novel secondary metabolites in actinomycetes by co-culture with mycolic acid-containing bacteria2018

    • Author(s)
      Hoshino Shotaro、Onaka Hiroyasu、Abe Ikuro
    • Journal Title

      Journal of Industrial Microbiology & Biotechnology

      Volume: 46 Issue: 3-4 Pages: 363-374

    • DOI

      10.1007/s10295-018-2100-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure function and engineering of multifunctional non-heme iron dependent oxygenases in fungal meroterpenoid biosynthesis2018

    • Author(s)
      Nakashima Yu、Mori Takahiro、Nakamura Hitomi、Awakawa Takayoshi、Hoshino Shotaro、Senda Miki、Senda Toshiya、Abe Ikuro
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 1-10

    • DOI

      10.1038/s41467-017-02371-w

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Umezawamides, new bioactive polycyclic tetramate macrolactams isolated from a combined-culture of Umezawaea sp. and mycolic acid-containing bacterium2018

    • Author(s)
      Hoshino Shotaro、Wong Chin Piow、Ozeki Masahiro、Zhang Huiping、Hayashi Fumiaki、Awakawa Takayoshi、Asamizu Shumpei、Onaka Hiroyasu、Abe Ikuro
    • Journal Title

      The Journal of Antibiotics

      Volume: 71 Issue: 7 Pages: 653-657

    • DOI

      10.1038/s41429-018-0040-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Two new pyrrolo-2-aminoimidazoles, Clathriroles A and B, from marine sponge, Clathria prolifera collected in Myanmar2018

    • Author(s)
      Woo, S.-Y., Win, N. N., Wong, C. P., Ito, T., Hoshino, S., Ngwe, H., Aye, A. A., Han, N., Zhang, H., Hayashi, F., Abe, I., Morita, H.
    • Journal Title

      J. Nat. Med.,

      Volume: 72 Issue: 3 Pages: 803-807

    • DOI

      10.1007/s11418-018-1205-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mirilactams C-E, novel polycyclic macrolactams isolated from combined-culture of Actinosynnema mirum NBRC 14064 and mycolic acid-contanining bacterium2018

    • Author(s)
      Hoshino Shotaro、Ozeki Masahiro、Ozeki Masahiro、Zhang Huiping、Hayashi Fumiaki、Awakawa Takayoshi、Morita Hiroyuki、Onaka Hiroyasu、Abe Ikuro
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 66

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis for the unusual ring reconstruction in fungal meroterpenoid biogenesis2017

    • Author(s)
      Mori Takahiro、Iwabuchi Taiki、Hoshino Shotaro、Wang Hang、Matsuda Yudai、Abe Ikuro
    • Journal Title

      Nature Chemical Biology

      Volume: 13 Issue: 10 Pages: 1066-1073

    • DOI

      10.1038/nchembio.2443

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mycolic Acid Containing Bacterium Stimulates Tandem Cyclization of Polyene Macrolactam in a Lake Sediment Derived Rare Actinomycete2017

    • Author(s)
      Hoshino Shotaro、Okada Masahiro、Awakawa Takayoshi、Asamizu Shumpei、Onaka Hiroyasu、Abe Ikuro
    • Journal Title

      Organic Letters

      Volume: 19 Issue: 18 Pages: 4992-4995

    • DOI

      10.1021/acs.orglett.7b02508

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Manipulation of prenylation reactions by structure-based engineering of bacterial indolactam prenyltransferases2016

    • Author(s)
      T. Mori, L. Zhang, T. Awakawa, S. Hoshino, M. Okada, H. Morita, I. Abe
    • Journal Title

      Nature Communications

      Volume: 7 Issue: 1 Pages: 10849-10851

    • DOI

      10.1038/ncomms10849

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genome-based discovery of an unprecedented cyclization mode in fungal sesterterpenoids biosynthesis2016

    • Author(s)
      Okada, M., Matsuda, Y., Mitsuhashi, T., Hoshino, S., Mori, T., Nakagawa, K., Quan, Z., Qin, B., Zhang, H., Hayashi, F., Kawaide, H., Abe, I.
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 138 Issue: 31 Pages: 10011-10018

    • DOI

      10.1021/jacs.6b05799

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural diversification of lyngbyatoxin A by host-dependent heterologous expression of the tleABC biosynthetic gene cluster2016

    • Author(s)
      Zhang, L., Hoshino, S., Awakawa, T., Wakimoto, T., Abe, I.
    • Journal Title

      ChemBioChem

      Volume: 17 Issue: 15 Pages: 1407-1411

    • DOI

      10.1002/cbic.201600229

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 希少放線菌Streptoalloteichus tenebrarius NBRC 16177株が産生する二次代謝産物に関する研究2019

    • Author(s)
      星野翔太郎、淡川孝義、三橋隆章、Wong Chin Piow、森田洋行、阿部郁朗
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 希少放線菌に対する複合培養法の適用と新規二次代謝産物の獲得2018

    • Author(s)
      星野翔太郎、淡川孝義、尾仲宏康、阿部郁朗
    • Organizer
      第33回日本放線菌学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ACTIVATION OF SILENT BIOSYNTHETIC GENES OF RARE-ACTINOMYCETES BY COMBINED-CULTURE WITH MYCOLIC ACID-CONTAINING BACTERIUM2018

    • Author(s)
      Shotaro Hoshino, Takayoshi Awakawa, Hiroyasu Onaka, Ikuro Abe
    • Organizer
      ASP 2018 annual meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 複合培養法を利用した希少放線菌からの生物活性物質探索2018

    • Author(s)
      星野翔太郎、淡川孝義、浅水俊平、尾仲宏康、阿部郁朗
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Discovery of Novel Antibiotics from Rare-Actinomycetes Using Combined-Culture Strategy2018

    • Author(s)
      Shotaro Hoshino, Takayoshi Awakawa, Hiroyasu Onaka, Ikuro Abe
    • Organizer
      Natural Product Discovery and Development in the Genomic Era
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 放線菌における翻訳後修飾を担うisoprenyl tryptophan synthaseの探索及び機能解明研究2017

    • Author(s)
      星野 翔太郎, 岡田正弘, 淡川孝義, 阿部郁朗
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      東北大学川内北キャンパス (宮城県仙台市青葉区)
    • Year and Date
      2017-03-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 複合培養を利用した希少放線菌における潜在的二次代謝能の覚醒2017

    • Author(s)
      星野翔太郎、張恵平、林文晶、尾仲宏康、阿部郁朗
    • Organizer
      第32回 日本放線菌学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 複合培養法を用いた希少放線菌からの新規生物活性物質探索2017

    • Author(s)
      星野翔太郎、張恵平、林文晶、尾仲宏康、阿部郁朗
    • Organizer
      日本生薬学会第64回年会、東邦大学習志野キャンパス、千葉県船橋市、2017/9/10.
    • Related Report
      2017 Annual Research Report
  • [Presentation] 複合培養法を用いた希少放線菌からの医薬品資源探索2017

    • Author(s)
      星野翔太郎、淡川孝義、岡田正弘、浅水俊平、尾仲宏康、 阿部郁朗
    • Organizer
      第7回食品薬学シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Application of Combined Culture Method to Rare Actinomycetes2017

    • Author(s)
      Shotaro Hoshino, Masahiro Okada, Hiroyasu Onaka, Ikuro Abe
    • Organizer
      18th International Symposium on the Biology of Actinomycetes
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 放線菌における翻訳後修飾を担うトリプトファンイソプレニル化酵素の探索及び機能解明研究2016

    • Author(s)
      星野 翔太郎, 岡田正弘, 淡川孝義, 阿部郁朗
    • Organizer
      第21回天然薬物の開発と応用シンポジウム
    • Place of Presentation
      千葉大学薬学部120周年記念講堂 (千葉県千葉市中央区)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] 放線菌における翻訳後修飾によるトリプトファンのイソプレニル化酵素の探索及び機能解析2016

    • Author(s)
      星野 翔太郎, 岡田正弘, 淡川孝義, 阿部郁朗
    • Organizer
      2016年度日本放線菌学会大会
    • Place of Presentation
      東京大学農学部弥生講堂 一条ホール (東京都文京区)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report

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Published: 2016-05-17   Modified: 2024-03-26  

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