根毛細胞の可塑的な伸長成長を支える転写制御ネットワークの解明

• Project/Area Number: 16J07464
• Research Category: Grant-in-Aid for JSPS Fellows
• Allocation Type: Single-year Grants
• Section: 国内
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: 柴田 美智太郎 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員(PD) (20734817)
• Project Period (FY): 2016-04-22 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 転写制御ネットワーク / 根毛 / 環境応答 / 転写因子 / 遺伝子制御ネットワーク

Outline of Annual Research Achievements

根毛は、植物の根の表面にある毛のような組織である。根の表面積を大きくすることで、土壌からの水や栄養素の効率的な吸収に寄与する。根毛の長さは植物の生育する環境に応答し、一般に栄養が豊富な環境では根毛の成長が抑制され、逆に栄養が不足している環境下は根毛の成長が促進される。すなわち、植物は自身の置かれた環境に対して適切な長さを調節していると考えられる。一方で、その分子機構の多くは未解明である。本研究では、その根毛の柔軟な成長制御機構を転写制御という観点から明らかにするために、転写因子による根毛の成長制御機構の解明を行ってきた。
本研究の初年度では、根毛の成長を制御する転写因子のスクリーニングを実施し、未解明の転写因子-遺伝子の制御関係を多数見出した。そして二年目では、GTL1というトリヘリックス型の転写因子に着目した解析を行い、GTL1がRSL4という転写因子の発現を抑制することで根毛の成長を負に制御することを明らかとした (Shibata et al., 2018)。最終年度となる3年目では、初年度に行ったスクリーニングのさらなる検証を進め、根毛のリン酸飢餓応答に重要な役割を担う転写因子を同定したほか、様々な環境シグナルを統合する役割を担う転写因子を発見した。これらの転写因子はまさしく本研究で同定を目指す環境シグナルに応答し根毛の成長を制御する転写因子であり、本研究計画が期待通り進行したことを示している。さらに３年間の集大成として、自分自身の研究成果だけでなく根毛の成長制御に関わる転写因子および転写制御について網羅したレビューを書き上げて発表した（Shibata & Sugimoto, 2019）。

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] UC Davis/NC State University(米国)
• [Int'l Joint Research] UC Davis/North Calorina/Duke(米国)
• [Int'l Joint Research] カリフォルニア大学デイビス校(米国)
• [Journal Article] A gene regulatory network for root hair development (2019)
  • Author(s): Michitaro Shibata, Keiko Sugimoto
  • Journal Title: Journal of Plant Research Volume: 132 Issue: 3 Pages: 301-309
  • DOI: 10.1007/s10265-019-01100-2
  • Peer Reviewed / Open Access
• [Journal Article] Toward a multifaceted understanding of plants’ adaptation mechanisms (2019)
  • Author(s): Michitaro Shibata, Junro Mogami
  • Journal Title: Journal of Plant Research Volume: 132 Issue: 3 Pages: 299-299
  • DOI: 10.1007/s10265-019-01101-1
• [Journal Article] A Gene Regulatory Network for Cellular Reprogramming in Plant Regeneration (2018)
  • Author(s): Ikeuchi M, Shibata M, Rymen B, Iwase A, Bagman AM, Watt L, Coleman D, Favero DS, Takahashi T, Ahnert SE, Brady SM, Sugimoto K
  • Journal Title: Plant and Cell Physiology Volume: 59 Issue: 4 Pages: 770-782
  • DOI: 10.1093/pcp/pcy013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] GTL1 and DF1 regulate root hair growth through transcriptional repression of ROOT HAIR DEFECTIVE 6-LIKE 4 in Arabidopsis (2018)
  • Author(s): Shibata Michitaro、Breuer Christian、Kawamura Ayako、Clark Natalie M.、Rymen Bart、Braidwood Luke、Morohashi Kengo、Busch Wolfgang、Benfey Philip N.、Sozzani Rosangela、Sugimoto Keiko
  • Journal Title: Development Volume: 145 Issue: 3 Pages: 159707-159707
  • DOI: 10.1242/dev.159707
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular networks orchestrating plant cell growth. (2017)
  • Author(s): Franciosini, A., Rymen, B., Shibata, M., Favero, D.S., and Sugimoto, K.
  • Journal Title: Current opinion in plant biology Volume: 35 Pages: 98-104
  • DOI: 10.1016/j.pbi.2016.11.010
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] ABA Suppresses Root Hair Growth via the OBP4 Transcriptional Regulator. (2017)
  • Author(s): Rymen, B., Kawamura, A., Schafer, S., Breuer, C., Iwase, A., Shibata, M., Ikeda, M., Mitsuda, N., Koncz, C., Ohme-Takagi, M., Matsui, M., and Sugimoto, K.
  • Journal Title: Plant physiology Volume: 173 Issue: 3 Pages: 1750-1762
  • DOI: 10.1104/pp.16.01945
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] A transcriptional cascade of root hair development in response to environmental signals (2019)
  • Author(s): Michitaro Shibata, Ayako Kawamura, Keiko Sugimoto
  • Organizer: 第60回日本植物生理学会
• [Presentation] FINE TUNING OF ROOT HAIR GROWTH BY A TRANSCRIPTIONAL REGULATORY NETWORK (2018)
  • Author(s): Michitaro Shibata, Christian Breuer, Ayako Kawamura, Bart Rymen, Lewis Watt, Natalie M. Clark Luke Braidwood, Rosangela Sozzani, Philip N. Benfey, Keiko Sugimoto
  • Organizer: International Conference on Arabidopsis Research (ICAR)
  • Int'l Joint Research
• [Presentation] Physiological functions of the regulation of root hair growth by a transcription factor GTL1 (2018)
  • Author(s): Michitaro Shibata, Ayako Kawamura, Keiko Sugimoto
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] Two transcription factors govern root hair growth in Arabidopsis (2017)
  • Author(s): Shibata M., Breuer C., Kawamura A., Rymen B., Watt L., Clark NM., Braidwood L., Sozzani R., Benfey PN., Sugimoto K.
  • Organizer: 植物生理学会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
• [Presentation] The regulatory mechanisms for root hair growth of Arabidopsis by two transcription factors, GTL1 and RSL4 (2017)
  • Author(s): Michitaro Shibata, Christian Breuer, Ayako Kawamura, Bart Rymen, Lewis Watt, Natalie M. Clark, Luke Braidwood, Rosangela Sozzani, Siobhan M. Brady, Philip N. Benfey, Keiko Sugimoto
  • Organizer: International Symposium on Imaging Frontier 2017
  • Int'l Joint Research
• [Presentation] A trihelix transcription factor GTL1 regulates root hair growth through transcriptional repression of RSL4 (2017)
  • Author(s): Michitaro Shibata, Christian Breuer, Ayako Kawamura, Bart Rymen, Lewis Watt, Natalie M. Clark, Luke Braidwood, Rosangela Sozzani, Siobhan M. Brady, Philip N. Benfey, Keiko Sugimoto
  • Organizer: FASEB -Mechanisms in Plant Development
  • Int'l Joint Research
• [Presentation] The gene regulatory network of root hair development (2017)
  • Author(s): Michitaro Shibata, Christian Breuer, Ayako Kawamura, Bart Rymen, Lewis Watt, Natalie M. Clark, Luke Braidwood, Rosangela Sozzani, Siobhan M. Brady, Philip N. Benfey, Keiko Sugimoto
  • Organizer: 日本植物学会第81回大会 国際シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] A trihelix transcription factor GTL1 is the negative regulator for root hair growth (2017)
  • Author(s): Michitaro Shibata, Christian Breuer, Ayako Kawamura, Bart Rymen, Lewis Watt, Natalie M. Clark, Luke Braidwood, Rosangela Sozzani, Siobhan M. Brady, Philip N. Benfey, Keiko Sugimoto
  • Organizer: Taiwan-Japan Plant Biology 2017
  • Int'l Joint Research
• [Presentation] GTL1, a Trihelix Transcription Factor, is a Novel Repressor of Root Hair Growth in Arabidopsis thaliana (2017)
  • Author(s): Michitaro Shibata, Christian Breuer, Ayako Kawamura, Bart Rymen, Lewis Watt, Natalie M. Clark, Luke Braidwood, Rosangela Sozzani, Siobhan M. Brady, Philip N. Benfey, Keiko Sugimoto
  • Organizer: Marchantia Workshop 2017
  • Int'l Joint Research
• [Presentation] The analysis of gene regulatory networks in Arabidopsis based on yeast one-hybrid screening (2017)
  • Author(s): Shibata M.
  • Organizer: The 3rd CSRS-ITbM Joint Workshop
  • Place of Presentation: 名古屋大学
• [Presentation] A trihelix transcription factor GTL1 regulates root hair development in Arabidopsis (2016)
  • Author(s): Michitaro Shibata, Christian Breuer, Ayako Kawamura, Bart Rymen, Lewis Watt, Natalie M. Clark, Luke Braidwood, Rosangela Sozzani, Philip N. Benfey, Keiko Sugimoto
  • Organizer: Cold Spring Harbor Asia Conference
  • Place of Presentation: Awaji Yumebutai International Conference Centre
  • Year and Date: 2016-11-29
  • Int'l Joint Research
• [Presentation] Yeast one-hybrid screening for cellular reprograming (2016)
  • Author(s): 柴田美智太郎
  • Organizer: 新学術領域研究「環境記憶統合」第2回若手の会
  • Place of Presentation: 熱海・KKRホテル熱海
  • Year and Date: 2016-10-12
• [Presentation] Root hair development of Arabidopsis in response to environmental demands (2016)
  • Author(s): Shibata M.
  • Organizer: UC Davis Postdoctoral Research Symposium
  • Place of Presentation: UC Davis California USA
  • Int'l Joint Research
• [Remarks] 細胞機能研究チームのホームページ
  • URL: http://cellfunction.riken.jp/
• [Remarks] 細胞機能研究チームのホームページ
  • URL: http://cellfunction.riken.jp/index.html
• [Remarks] 理化学研究所プレスリリース
  • URL: http://www.riken.jp/pr/press/2018/20180214_2/
• [Remarks] 理化学研究所プレスリリース
  • URL: http://www.riken.jp/pr/press/2018/20180215_1/
• [Remarks] 理化学研究所 Cell Function Research Teamのホームページ
  • URL: http://cellfunction.riken.jp/