Analysis of spectral opponent mechanism in the optic lobe of the butterfly, Papilio xuthus
| Project/Area Number |
16J07688
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | The Graduate University for Advanced Studies |
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Principal Investigator |
Chen PeiーJu (2017-2018) 総合研究大学院大学, 先導科学研究科, 特別研究員(DC1)
CHEN PEIーJU (2016) 総合研究大学院大学, 先導科学研究科, 特別研究員(DC1)
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| Project Period (FY) |
2016-04-22 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2018: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2017: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2016: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | butterfly / electrophysiology / histaminergic channel / immunohistochemistry / lamina monopolar cell / photoreceptor / spectral opponency / color vision / vision / insect / optic lobe / histamine channel / synapse |
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| Outline of Annual Research Achievements |
Spectral opponent responses have been observed at the level of photoreceptors in butterflies. To explain its mechanism, I proposed a “histamine hypothesis”: the opponency may be attributed to the antagonistic interaction between photoreceptors via the activation of histaminergic channels.
Immunolocalization on histamine-gated chloride channels in Papilio xuthus suggested that the lamina monopolar cells (LMCs) and photoreceptors express PxHCLA and PxHCLB, respectively. Using sharp microelectrode recording with current injections, the responses of both LMCs and spectrally opponent photoreceptors could be reversed when the membrane potential was close to the equilibrium potential of chloride ions.
Intracellular recording in the lamina showed that LMCs are divided into non-spectrally opponent and spectrally opponent ones. The former includes three spectrally heterogeneous classes, which are ommatidial type dependent. It suggests that LMCs seem to integrate chromatic signals from the photoreceptors in the same ommatidium via PxHCLA. I also correlated LMC spectral types with morphological types. The findings of spectrally opponent LMCs and variable spectral classes provide a new insight into the role of LMCs in color signaling circuitry. In addition, several classes of spectrally opponent photoreceptors were recorded. The recorded opponent responses can be well explained by the ommatidial type-specific inter-photoreceptor synaptic connections via PxHCLB. The results presented in this project will be a springboard for future studies of early chromatic processing in insects.
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| Research Progress Status |
平成30年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
平成30年度が最終年度であるため、記入しない。
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Report
(3 results)
Research Products
(16 results)
