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マダニ過酸化水素消去酵素に着目した高免疫効率型の新規抗マダニワクチン開発への挑戦

Research Project

Project/Area Number 16J08221
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Veterinary medical science
Research InstitutionYamaguchi University

Principal Investigator

草木迫 浩大  山口大学, 連合獣医学研究科, 特別研究員(DC2)

Project Period (FY) 2016-04-22 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2017: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2016: ¥700,000 (Direct Cost: ¥700,000)
Keywordsマダニ / ペルオキシレドキシン / ワクチン候補分子 / Th2免疫経路 / 過酸化水素 / 遺伝子ノックダウン
Outline of Annual Research Achievements

平成28年度の研究成果から、マダニペルオキシレドキシン (HlPrx2) は、マダニの吸血、産卵並びに過酸化水素濃度の制御に重要な分子であった。このため、組換え HlPrx2 (rHlPrx2) で免疫した宿主動物でマダニを吸血させ、マダニの吸血、発育並びに産卵に及ぼす影響を評価し、HlPrx2 がワクチン標的候補分子となるか検証した。実験群として、rHlPrx2 のみ免疫群、rHlPrx2 と同量のフロイント不完全アジュバント (IFA) を混合し免疫した群、陰性対照として、rHlPrx2 の代わりにリン酸緩衝液 (PBS) を使用した2群の4群を設定した。抗体価は、ELISA を用いて測定した。免疫動物は、日本白色ウサギ並びに BALB/c マウスを用いた。ウサギ並びにマウスにおいて、rHlPrx2 のみ免疫でも十分な抗体価を誘導でき、さらに、宿主体内にできた特異的抗体が、マダニ吸血に反応した。特に、免疫マウスにおいて、rHlPrx2 のみ免疫群で Th2 免疫経路のマーカーである IgG1 を特異的に誘導した。しかし、実際に免疫ウサギで雌成ダニを吸血させたところ、吸血並びに産卵への顕著な影響は認められず、また免疫マウスで若ダニを吸血させても、若ダニの吸血並びにその後の発育への顕著な影響は認められなかった。
これらの結果から、宿主への rHlPrx2 免疫はマダニの吸血、発育並びに産卵に大きな影響を及ぼさないことが示唆された。しかし、rHlPrx2 のみの免疫でも宿主に十分な抗体価を誘導でき、さらに Th2 免疫経路のマーカーである IgG1 抗体を特異的に誘導したことから、rHlPrx2 は Th2 免疫経路賦活型の生物由来アジュバントとしての応用の可能性が考えられた。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (28 results)

All 2018 2017 2016 Other

All Journal Article (8 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 5 results) Presentation (17 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Peroxiredoxins are important for the regulation of hydrogen peroxide concentrations in ticks and tick cell line2018

    • Author(s)
      Kusakisako, K., Hernandez, E. P., Talactac, M. R., Yoshii, K., Umemiya-Shirafuji, R., Fujisaki, K. and Tanaka, T.
    • Journal Title

      Ticks Tick Borne Dis.

      Volume: In press Issue: 4 Pages: 870-881

    • DOI

      10.1016/j.ttbdis.2018.03.016

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Evaluation of vaccine potential of 2-Cys peroxiredoxin from the hard tick Haemaphysalis longicornis2018

    • Author(s)
      Kusakisako, K., Miyata, T., Tsujio, M., Galay, R. L., Talactac, M. R., Hernandez, E. P., Fujisaki, K. and Tanaka, T.
    • Journal Title

      Exp. Appl. Acarol.

      Volume: 74 (1) Issue: 1 Pages: 73-84

    • DOI

      10.1007/s10493-018-0209-3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] マダニペルオキシレドキシンの役割 ―特に吸血における抗酸化機構と宿主の免疫応答について―2017

    • Author(s)
      草木迫 浩大、藤﨑 幸藏、田仲 哲也
    • Journal Title

      獣医寄生虫学会誌

      Volume: 16 Pages: 22-28

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Initial development of Babesia ovata in the tick midgut2017

    • Author(s)
      Maeda, H., Hatta, T., Alim, M. A., Tsubokawa, D., Mikami, F., Kusakisako, K., Matsubayashi, M., Umemiya-Shirafuji, R., Tsuji, N. and Tanaka, T.
    • Journal Title

      Vet. Prasitol.

      Volume: 233 Pages: 39-42

    • DOI

      10.1016/j.vetpar.2016.11.020

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis2017

    • Author(s)
      Talactac, M. R., Yada, Y., Yoshii, K., Hernandez, E. P., Kusakisako, K., Maeda, H., Galay, R. L., Fujisaki, K., Mochizuki, M. and Tanaka, T.
    • Journal Title

      Dev. Comp. Immunol.

      Volume: 68 Pages: 98-107

    • DOI

      10.1016/j.dci.2016.11.013

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 2-Cys peroxiredoxin is required in successful blood-feeding, reproduction, and antioxidant response in the hard tick Haemaphysalis longicornis2016

    • Author(s)
      Kusakisako K, Galay RL, Umemiya-Shirafuji R, Hernandez EP, Maeda H, Talactac MR, Tsuji N, Mochizuki M, Fujisaki K, Tanaka T.
    • Journal Title

      Parasites and Vectors

      Volume: 9 Issue: 1 Pages: 457-457

    • DOI

      10.1186/s13071-016-1748-2

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Improvement of the cryopreservation method for the Babesia gibsoni parasite by using commercial freezing media2016

    • Author(s)
      Kusakisako, K., Masatani, T., Yada Y., Talactac, M. R., Hernandez, E. P., Maeda, H., Mochizuki, M. and Tanaka, T.
    • Journal Title

      Parasitol. Int.

      Volume: In press Issue: 5 Pages: 532-535

    • DOI

      10.1016/j.parint.2016.02.012

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Induction of gene silencing in Haemaphysalis longicornis ticks through immersion in double-stranded RNA2016

    • Author(s)
      Galay, R. L., Hernandez, E. P., Talactac, M. R., Maeda, H., Kusakisako, K., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
    • Journal Title

      Ticks Tick Borne Dis.

      Volume: In press Issue: 5 Pages: 813-816

    • DOI

      10.1016/j.ttbdis.2016.03.018

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 2-Cys peroxiredoxin has an important role in blood feeding and reproduction through the regulation of hydrogen peroxide concentrations in the hard tick Haemaphysalis longicornis2018

    • Author(s)
      Kusakisako Kodai、Galay Remil Linggatong、Umemiya-Shirafuji Rika、Hernandez Emmanuel Pacia、Maeda Hiroki、Talactac Melbourne Rio、Tsuji Naotoshi、Mochizuki Masami、Fujisaki Kozo、Tanaka Tetsuya
    • Organizer
      The 9th Joint Symposium of Veterinary Research in East Asia
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Peroxiredoxins are important for blood feeding and reproduction through the regulation of H2O2 concentrations in the hard tick Haemaphysalis longicornis2017

    • Author(s)
      Kodai Kusakisako, Remil Linggatong Galay, Rika Umemiya-Shirafuji, Emmanuel Pacia Hernandez, Hiroki Maeda, Melbourne Rio Talactac, Naotoshi Tsuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
    • Organizer
      International Symposium in Veterinary Science
    • Place of Presentation
      Bogor, Indonesia
    • Year and Date
      2017-01-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The potential of recombinant ferritins as anti-tick vaccine against Haemaphysalis longicornis2017

    • Author(s)
      Remil Linggatong Galay, Takeshi Miyata, Rika Umemiya-Shirafuji, Hiroki Maeda, Kodai Kusakisako, Masami Mochizuki, KozoFujisaki, Tetsuya Tanaka
    • Organizer
      International Symposium in Veterinary Science
    • Place of Presentation
      Bogor, Indonesia
    • Year and Date
      2017-01-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interaction of different acaricides with the recombinant glutathione S-transferases of Haemaphysalis longicornis2017

    • Author(s)
      Emmanuel Pacia Hernandez, Kodai Kusakisako, Remil Linggatong Galay, Hiroki Maeda, Melbourne Rio Talactac, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
    • Organizer
      International Symposium in Veterinary Science
    • Place of Presentation
      Bogor, Indonesia
    • Year and Date
      2017-01-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation of the survival dynamics of Japan isolate thogoto virus in Haemaphysalis longicornis2017

    • Author(s)
      Melbourne Rio Talactac, Kentaro Yoshii, Emmanuel Pacia Hernandez, Kodai Kusakisako, Hiroki Maeda, Kozo Fujisaki, Masami Mochizuki, Tetsuya Tanaka,
    • Organizer
      International Symposium in Veterinary Science
    • Place of Presentation
      Bogor, Indonesia
    • Year and Date
      2017-01-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 哺乳類細胞におけるマダニアクチン遺伝子プロモーターを用いた外来遺伝子の発現2017

    • Author(s)
      諸熊 遥樹、草木迫 浩大、Talactac Melbourne Rio、Hernandez Emmanuel Pacia、田仲 哲也
    • Organizer
      第70回日本寄生虫学会南日本支部大会・第67回日本衛生動物学会南日本支部大会合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マダニペルオキシレドキシンの特性解明 (-ペルオキシレドキシンの性状解析並びに吸血・産卵に果たす役割について-)2017

    • Author(s)
      草木迫 浩大、正谷 達謄、宮田 健、Galay Remil Linggatong、白藤(梅宮) 梨可、前田 大輝、Talactac Melbourne Rio、Hernandez Emmanuel Pacia、辻 尚利、望月 雅美、藤崎 幸蔵、田仲 哲也
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ペルオキシレドキシンは連携して細胞内過酸化水素濃度を制御する2017

    • Author(s)
      草木迫 浩大、Hernandez Emmanuel Pacia、Talactac Melbourne Rio、好井 健太朗、田仲 哲也
    • Organizer
      第86回日本寄生虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Peroxiredoxins are important for blood feeding and reproduction through the regulation of hydrogen peroxide concentrations in the hard tick Haemaphysalis longicornis2017

    • Author(s)
      Kusakisako Kodai、Masatani Tatsunori、Miyata Takeshi、Galay Remil Linggatong、Umemiya-Shirafuji Rika、Hernandez Emmanuel Pacia、Maeda Hiroki、Talactac Melbourne Rio、Tsuji Naotoshi、Mochizuki Masami、Fujisaki Kozo、Tanaka Tetsuya
    • Organizer
      One Health 9th Tick and Tick-borne Pathogen Conference & 1st Asia Pacific Rickettsia Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] フタトゲチマダニへモリンフ由来ディフェンシンの同定とその抗菌作用2016

    • Author(s)
      屋田友里花, 草木迫浩大, Melbourne Rio Talactac, Emmanuel Pacia Hernandez, 前田大輝, 安藤匡子, 望月雅美, 藤崎幸蔵, 田仲哲也
    • Organizer
      第69回日本寄生虫学会南日本支部大会・第66回日本衛生動物学会南日本支部大会合同大会
    • Place of Presentation
      佐賀大学(佐賀県、佐賀市)
    • Year and Date
      2016-11-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] ダニ胚由来細胞における遺伝子発現ベクターの検証2016

    • Author(s)
      井戸明子, 正谷達謄, 好井健太朗, Remil Linggatong Galay, 草木迫浩大, Melbourne Rio Talactac, Emmanuel Pacia Hernandez, 望月雅美, 田仲哲也
    • Organizer
      第69回日本寄生虫学会南日本支部大会・第66回日本衛生動物学会南日本支部大会合同大会
    • Place of Presentation
      佐賀大学(佐賀県、佐賀市)
    • Year and Date
      2016-11-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] Experimental transmission of tick-borne encephalitis virus surrogate Langat virus by Haemaphysalis longicornis2016

    • Author(s)
      Melbourne Rio Talactac, Kentaro Yoshii, Emmanuel Pacia Hernandez, Kodai Kusakisako, Hiroki Maeda, Kozo Fujisaki, Masami Mochizuki, Tetsuya Tanaka
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道、札幌市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] マダニISE6細胞を用いた過酸化水素発生の可視化についての検討2016

    • Author(s)
      草木迫浩大, Melbourne Rio Talactac, Emmanuel Pacia Hernandez, 前田大輝, 好井健太朗, 望月雅美, 田仲哲也
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      日本大学(神奈川県、藤沢市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] フタトゲチマダニ体内での過酸化水素消去酵素ペルオキシレドキシンの役割について2016

    • Author(s)
      草木迫浩大, Remil Linggatong Galay, 白藤(梅宮)梨可, Emmanuel Pacia Hernandez, 前田大輝, Melbourne Rio Talactac, 辻尚利, 望月雅美, 藤崎幸蔵, 田仲哲也
    • Organizer
      第24回 Seminar on Acari-Desease Interface (SADI)
    • Place of Presentation
      休暇村指宿(鹿児島県、指宿市)
    • Year and Date
      2016-05-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] フタトゲチマダニの2本鎖RNAを用いた浸漬法による遺伝子抑制の確立2016

    • Author(s)
      田仲哲也, Remil Linggatong Galay, Emmanuel Pacia Hernandez, Melbourne Rio Talactac, 前田大輝, 草木迫浩大, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵
    • Organizer
      第24回 Seminar on Acari-Desease Interface (SADI)
    • Place of Presentation
      休暇村指宿(鹿児島県、指宿市)
    • Year and Date
      2016-05-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] Survival dynamics of tick-borne encephalitis virus surrogate Langat virus in Haemaphysalis longicornis2016

    • Author(s)
      Melbourne Rio Talactac, Kentaro Yoshii, Hiroki Maeda, Kodai Kusakisako, Emmanuel Pacia Hernandez, Kozo Fujisaki, Remil Linggatong Galay, Tetsuya Tanaka, Masami Mochizuki
    • Organizer
      第24回 Seminar on Acari-Desease Interface (SADI)
    • Place of Presentation
      休暇村指宿(鹿児島県、指宿市)
    • Year and Date
      2016-05-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] Identification and partial characterization of Haemaphysalis longicornis glutathione S- transferases (GST)2016

    • Author(s)
      Emmanuel Pacia Hernandez, Kodai Kusakisako, Hiroki Maeda, Remil Linggatong Galay, Melbourne Rio Talactac, Masami Mochizuki, Kozo Fujizaki, Tetsuya Tanaka
    • Organizer
      第24回 Seminar on Acari-Desease Interface (SADI)
    • Place of Presentation
      休暇村指宿(鹿児島県、指宿市)
    • Year and Date
      2016-05-27
    • Related Report
      2016 Annual Research Report
  • [Book] Ticks' Antioxidant Complex: A Defense Stronghold and a Potential Target for Their Control, Advances in Medicine and Biology Volume 116. (Editors: Leon V. Berhardt)2017

    • Author(s)
      Galay RL, Hernandez EP, Talactac MR, Kusakisako K, Fujisaki K, Tanaka T
    • Total Pages
      256
    • Publisher
      Nova Science Publishers
    • Related Report
      2016 Annual Research Report
  • [Remarks] 鹿児島大学 共同獣医学部 獣医学科 病態予防獣医学講座 感染症学分野

    • URL

      http://www.vet.kagoshima-u.ac.jp/kadai/V-Infection/sinkoukansen/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 鹿児島大学 共同獣医学部獣医学科 病態予防獣医学講座 感染症学分野

    • URL

      http://www.vet.kagoshima-u.ac.jp/kadai/V-Infection/sinkoukansen/index.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-05-17   Modified: 2024-03-26  

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