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膜通気バイオフィルムリアクターによる高濃度窒素排水の効率除去とN2O削減

Research Project

Project/Area Number 16J08490
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Environmental engineering and reduction of environmental burden
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Co ThiKinh (2017)  東京農工大学, 大学院工学府, 特別研究員(DC2)

CO THIKINH (2016)  東京農工大学, 大学院工学府, 特別研究員(DC2)

Project Period (FY) 2016-04-22 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2017: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2016: ¥900,000 (Direct Cost: ¥900,000)
KeywordsMABR / CBR / nitrous oxide emission / nitrite oxide emission / nosZ / co-diffusion biofilm / denitrifiers / Nitric oxide / Microelectrode / Nitrous oxide / Membrane-aerated biofilm
Outline of Annual Research Achievements

To treat ammonium rich wastewaters in conventional activated sludge systems, conventional nitrification and denitrification is highly cost for aeration and external organic matter. Besides, N2O emission has been an emergent issue for the past few years due to its significant greenhouse effect. N2O emission from wastewater treatment plants is considered as one of major anthropogenic sources, thus reducing N2O emission from WWTPs is important. My current studies focus on investigation the potential of a membrane-aerated biofilm reactor (MABR) for simultaneous nitrification and denitrification as a compact and cost-effective wastewater treatment technology with minimum N2O emission.
In order to understand the impact of C/N ratio in simultaneous nitrification and denitrification process in an MABR and a conventional biofilm reactor (CBR), we operated 2 reactors representative for MABR and CBR. We found that the counter diffusion biofilms in MABR emits 130 times lower N2O than co-diffusion biofilms in a CBR. The MABR for also had 20% higher oxygen utilization efficiency and 12% higher TN removal efficiency than that of CBR at the same operational conditions. The molecular microbiological analysis indicated higher abundance of denitrifying genes, especially nosZ genes, in the MABR versus the CBR, especially the presence of the genera Thauera, Rhizobium, Sphingobacteria, and Brevundimonas as potential N2O-reducing bacteria in the MABR. The works were published in top rank journals including Applied Microbiology and Biotechnology, Water Research, and Bioresource Technology.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (8 results)

All 2017 2016

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Counter-diffusion biofilms have lower N 2 O emissions than co-diffusion biofilms during simultaneous nitrification and denitrification: Insights from depth-profile analysis2017

    • Author(s)
      Kinh Co Thi、Suenaga Toshikazu、Hori Tomoyuki、Riya Shohei、Hosomi Masaaki、Smets Barth F.、Terada Akihiko
    • Journal Title

      Water Research

      Volume: 124 Pages: 363-371

    • DOI

      10.1016/j.watres.2017.07.058

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of hotspots for NO and N 2 O production and consumption in counter- and co-diffusion biofilms for simultaneous nitrification and denitrification2017

    • Author(s)
      Kinh Co Thi、Riya Shohei、Hosomi Masaaki、Terada Akihiko
    • Journal Title

      Bioresource Technology

      Volume: 245 Pages: 318-324

    • DOI

      10.1016/j.biortech.2017.08.051

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Free nitrous acid and pH determine the predominant ammonia-oxidizing bacteria and amount of N2O in a partial nitrifying reactor2017

    • Author(s)
      C.T. Kinh, J. Ahn, T. Suenaga, N. Sittivorakulpong, P. Noophan, T. Hori, S. Riya, M. Hosomi, A. Terada
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 101 Issue: 4 Pages: 1673-1683

    • DOI

      10.1007/s00253-016-7961-2

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Membrane-aerated biofilm reactor for simultaneous nitrification and denitrification mitigates N2O emission because of counter-current substrate diffusion geometry: Depth-profile analysis2017

    • Author(s)
      Co Thi Kinh, Toshikazu Suenaga, Tomoyuki Hori, Shohei Riya, Masaaki Hosomi, Akihiko Terada
    • Organizer
      Annual meeting of The Japanese Society of Water and Environment
    • Related Report
      2017 Annual Research Report
  • [Presentation] Counter-diffusion biofilms for simultaneous nitrification and denitrification reduce NO and N2O emissions: Insights from depth-profile analysis2017

    • Author(s)
      Co Thi Kinh, Toshikazu Suenaga, Tomoyuki Hori, Shohei Riya, Masaaki Hosomi, Barth Smets, Akihiko Terada
    • Organizer
      The 5th International Conference of Nitrification and Related Processes
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Influent carbon/nitrogen ratio determines N2O emission and spatial distribution of N2O reducing bacteria in a membrane-aerated biofilm2016

    • Author(s)
      Co Thi Kinh, Toshikazu Suenaga, Tomoyuki Hori, Shohei Riya, Masaaki Hosomi, Barth F. Smets, Akihiko Terada
    • Organizer
      Microbial Ecology in Water Engineering & Biofilms
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      2016-09-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A biofilm employing counter-current substrate diffusion geometry facilitates N2O mitigation: Proof of concept.2016

    • Author(s)
      Co Thi Kinh, Toshikazu Suenaga, Tomoyuki Hori, Shohei Riya , Masaaki Hosomi, Akihiko Terada
    • Organizer
      The 6th International conference of Water and Environment Technology
    • Place of Presentation
      Chuo University, Tokyo, Japan
    • Year and Date
      2016-08-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Depth profiles of nitrous oxide concentration and functional gene abundances in counter- and co- diffusion biofilms for nitrification and denitrification2016

    • Author(s)
      Akihiko Terada, Co Thi Kinh, Toshikazu Suekana, Tomoyuki Hori, Shohei Riya, Barth F. Smets, Masaaki Hosomi
    • Organizer
      WEF/IWA Nutrient Removal and Recovery 2016 Conference
    • Place of Presentation
      Denver, Colorado, USA
    • Year and Date
      2016-07-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2016-05-17   Modified: 2024-03-26  

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