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ネムリユスリカの無代謝休眠における遺伝子発現ネットワークの解析

Research Project

Project/Area Number 16J09151
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Insect science
Research InstitutionFukushima National College of Technology

Principal Investigator

十亀 陽一郎  福島工業高等専門学校, 物質工学科, 特命助教

Project Period (FY) 2016-04-22 – 2019-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords遺伝子発現 / 昆虫細胞 / 無代謝休眠
Outline of Annual Research Achievements

ネムリユスリカの無代謝休眠における遺伝子発現ネットワークの解析
ほとんどの生物は、水がない状態で生存することは不可能であるが、ネムリユスリカをはじめとする一部の生物は、乾燥に対する適応戦略として無代謝休眠を行う。無代謝休眠の状態では、生体はカラカラに干からびた状態になり(代謝は停止している)、乾燥をはじめ様々な環境ストレスに対する耐性を獲得する。本研究では、ネムリユスリカの無代謝休眠過程のメカニズムを明らかにするため、無代謝休眠過程で発現調節される遺伝子とその制御機構を明らかにすることを目的としている。
本年度は、ネムリユスリカの培養細胞を実験に用い、ネムリユスリカの培養細胞で外来遺伝子を発現・サイレングさせる実験系の構築を行った。これらの実験系の構築は、ネムリユスリカを用いた無代謝休眠の研究で広く利用できることが期待される。

Research Progress Status

翌年度、交付申請を辞退するため、記入しない。

Strategy for Future Research Activity

翌年度、交付申請を辞退するため、記入しない。

Report

(1 results)
  • 2016 Annual Research Report
  • Research Products

    (12 results)

All 2017 2016

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 5 results)

  • [Journal Article] Current findings on the molecular mechanisms underlying anhydrobiosis in Polyprdilum vanderplanki2017

    • Author(s)
      Sogame Y, Kikawada T
    • Journal Title

      Current Opinion in Insect Science

      Volume: 19 Pages: 16-21

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Establishment of gene transfer and gene silencing methods in a desiccation‐tolerant cell line, Pv112017

    • Author(s)
      Yoichiro Sogame, Jun Okada, Shingo Kikuta, Yugo Miyata, Richard Cornette, Oleg Gusev, Takahiro Kikawada
    • Journal Title

      Extremophiles

      Volume: 21 Issue: 1 Pages: 65-72

    • DOI

      10.1007/s00792-016-0880-4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Morphogenetic and molecular analyses of cyst wall components in the ciliated protozoan Colpoda cucullus Nag-1.2017

    • Author(s)
      Funadani R*, Sogame Y*,(*equal contributor) Kojima K, Takeshita T, Yamamoto K, Tsujizono T, Suizu F, Miyata S, Yagyu KI, Suzuki T, Arikawa M, Matsuoka T
    • Journal Title

      FEMS microbiology letters

      Volume: 363 Issue: 18 Pages: fnw203-fnw203

    • DOI

      10.1093/femsle/fnw203

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Genome Editing in a Cell Line of the Sleeping Chironomid by Using the CRISPR/Cas9 System2016

    • Author(s)
      Miyata Y, Sogame Y, Tokumoto S, Sakurai M, Kikawada T
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      神奈川
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] The sleeping chironomid as a model organism to investigate molecular mechanisms underlying anhydrobiosis2016

    • Author(s)
      Kikawada T, Miyata Y, Sogame Y, Cornrtte R, Gusev O
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      神奈川
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 繊毛虫コルポーダ Colpoda cucullus における休眠シスト壁形成に関する研究2016

    • Author(s)
      舟谷 亮二, 十亀 陽一郎, 小嶋 克彦, 竹下 敏一, 山本 圭祐, 辻囿 智哉, 水津 太, 宮田 清司, 矢生 健一, 鈴木 知彦, 有川 幹彦, 松岡 達臣
    • Organizer
      第49回 日本原生生物学会
    • Place of Presentation
      岡山
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Identification of a strong promoter in cultured cells of Polypedilum vanderplanki as a tool to reveal molecular background underlying anhydrobiosis2016

    • Author(s)
      Sogame Y, Okada J, Deviatiiarov R, Kikuta S,Cornette R, Gusev O, Kikawada T
    • Organizer
      25th International Congress of Entomology
    • Place of Presentation
      アメリカ
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The first edition of mutagenesis by CRISPR/Cas in the extreme desiccation tolerant cultured cell.2016

    • Author(s)
      Kikawada T, Miyata Y, Sogame Y, Furusawa T, Kikuta S, Cornette R, Gusev O
    • Organizer
      10th International Conference On Bioinformatics of Genome Regulation and Structure Systems Biology
    • Place of Presentation
      ロシア
    • Year and Date
      2016-08-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analyses on the mechanism of induction of anhydrobiosis in the midge Polypedilum vanderplanki2016

    • Author(s)
      Cornette R, Iwata K-I, Kikuta S, Sogame Y, Okuda T, Kikawada T
    • Organizer
      10th International Conference On Bioinformatics of Genome Regulation and Structure Systems Biology
    • Place of Presentation
      ロシア
    • Year and Date
      2016-08-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of a powerful constitutive promoter in culture cells of Polypedilum vanderplanki.2016

    • Author(s)
      Sogame Y, Miyata Y, Deviatiiarov R, Kikuta S, Cornette R, Gusev O, Furusawa T, Kikawada T
    • Organizer
      10th International Conference On Bioinformatics of Genome Regulation and Structure Systems Biology
    • Place of Presentation
      ロシア
    • Year and Date
      2016-08-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Anhydro-preservation of exogenously-expressed desiccation-sensitive enzyme Luciferase using insect cells2016

    • Author(s)
      Kikuta S, Watanabe S, Gusev O, Sogame Y, Cornett R, Kikawada T
    • Organizer
      10th International Conference On Bioinformatics of Genome Regulation and Structure Systems Biology
    • Place of Presentation
      ロシア
    • Year and Date
      2016-08-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 乾燥状態のネムリユスリカ由来培養細胞は外来性酵素を保護する2016

    • Author(s)
      渡辺俊介, 菊田 真吾, Oleg Gusev, 十亀陽一郎, 佐藤令一, Richard Cornette, 黄川田隆洋
    • Organizer
      第61回低温生物工学会大会
    • Place of Presentation
      埼玉
    • Year and Date
      2016-06-25
    • Related Report
      2016 Annual Research Report

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Published: 2016-05-17   Modified: 2024-03-26  

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