• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

臨界ゲルクラスターを用いた巨大生体分子分離媒体の創製

Research Project

Project/Area Number 16J09442
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Polymer/Textile materials
Research InstitutionThe University of Tokyo

Principal Investigator

Khairulina Kateryna (2017)  東京大学, 工学系研究科, 特別研究員(DC2)

KHAIRULINA KATERYNA (2016)  東京大学, 大学院工学系研究科(工学部), 特別研究員(DC2)

Project Period (FY) 2016-04-22 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2017: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2016: ¥700,000 (Direct Cost: ¥700,000)
Keywordspolymer gel / polyelectrolytes / dynamics / electrophoresis
Outline of Annual Research Achievements

Mobility of charged solutes in polymer networks is governed by diffusion and electrically driven migration, the latter one excessively utilized in gel electrophoresis. However, the physical mechanism of the migration is still not clear yet due to the uncontrollability of the number of structural parameters influencing migration, resulting in no clear understanding of solute-matrix interactions. In this thesis we used tetra-PEG gel as a model system and investigated the influence of structural parameters on migration of DNA molecules under constant electric field conditions. The effects of final network connectivity as well as induced electroosmosis were clarified. In addition, we propose a novel PEG gel electrophoresis strategy for separation of DNA fragments and low molecular weight compounds by doping graphene oxide (GO) into tetra-PEG gel. The results show that the addition of GO into PEG gel significantly improved the separation resolution of DNA fragments and considerably decreased electrophoretic mobility of small charged dyes ethidium bromide and rhodamine 6G. These results provide promising potential for graphene and its derivate utilized in various electrophoresis techniques for separation and detection of DNA fragments and other charged molecules.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (7 results)

All 2017 2016 2015 2014 2013

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Hydrogels with tuned electro-osmosys:potential model system to understand electro-kinetic transport in biological tissues2017

    • Author(s)
      Khairulina, K., Chung, U., Sakai, T.
    • Journal Title

      Journal of Materials Chemistry B

      Volume: 14 Issue: 23 Pages: 45674672-45674672

    • DOI

      10.1039/c7tb00064b

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Electrophoretic mobility of semi-flexible double-stranded DNA in defect-controlled polymer networks: mechanism investigation and role of structural parameters2015

    • Author(s)
      Khairulina, K; Li, X; Nishi, K; Shibayama, M; Chung, U; Sakai, T.
    • Journal Title

      J. Chem. Phys.

      Volume: 142 Issue: 23 Pages: 234904-234904

    • DOI

      10.1063/1.4922367

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Electrophoretic Mobility of Double-Stranded DNA in Polymer Solutions and Gels with Tuned Structures2014

    • Author(s)
      Xiang Li, Kateryna Khairulina, Ung-il Chung, and Takamasa Sakai
    • Journal Title

      Macromolecules

      Volume: 47 Issue: 11 Pages: 3582-3586

    • DOI

      10.1021/ma500661r

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Migration behavior of rod-like dsDNA under electric field in homogeneous polymer networks2013

    • Author(s)
      X. Li, K. Khairulina, U. Chung, and T. Sakai
    • Journal Title

      Macromolecules(ACS Publications)

      Volume: 46 Issue: 21 Pages: 8657-8663

    • DOI

      10.1021/ma401827g

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] Mobility of low molecular weight compounds and dsDNA in graphene oxide-PEG composite hydrogels2017

    • Author(s)
      Khairulina Kateryna, Yuta Nishina, Ung-il Chung, Takamasa Sakai
    • Organizer
      11th International Gel Symposium “GelSympo 2017"
    • Place of Presentation
      Nikon University, Japan
    • Year and Date
      2017-03-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Graphene oxide PEG hydrogels as efficient dye absorbents2016

    • Author(s)
      Khairulina Kateryna, Yuta Nishina, Ung-il Chung, Takamasa Sakai
    • Organizer
      International Polymer Conference (IPC)
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2016-12-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Electrophoretic mobility of dsDNA in polymer gels with tuned structures2016

    • Author(s)
      Khairulina Kateryna, Ung-il Chung, Takamasa Sakai
    • Organizer
      Polymer Gels and Networks 2016 (PN&G)
    • Place of Presentation
      KTH, Stockholm
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2016-05-17   Modified: 2024-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi