• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of biological response of heavy-ion radiation using microbeam irradiation with specially designed irradiation dish

Research Project

Project/Area Number 16K00552
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Risk sciences of radiation and chemicals
Research InstitutionNational Institutes for Quantum and Radiological Science and Technology

Principal Investigator

FUNAYAMA Tomoo  国立研究開発法人量子科学技術研究開発機構, 高崎量子応用研究所 放射線生物応用研究部, 上席研究員(定常) (40354956)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywordsマイクロビーム / 重イオン / 高LET / 重イオンヒット効果 / バイスタンダー効果
Outline of Final Research Achievements

In this study, we developed a specially-designed cell irradiation dish for analyzing the distance limitation of intercellular communication in radiation-induced bystander effect, which is difficult to carry out with the conventional general-purpose microbeam irradiation dish. A cover glass to be a cell attaching surface was adhered to a wall component in which grooves having a width of 2 mm were arranged in a maze shape designed using three-dimensional CAD software. To improve cell attachment efficiency on a cover glass, a treatment with a vacuum plasma was performed. HeLa cells were inoculated in a developed specially-designed cell dish, thereafter, placed on a cell targeting system of a collimating heavy-ion microbeam system of QST-Takasaki. We confirmed that the cells attached in the dish were observed enough clear for targeting, and was able to irradiate with defined number of carbon ion microbeam.

Academic Significance and Societal Importance of the Research Achievements

重イオン特有の生物効果の解明は、その応用である重粒子線がん治療やイオンビーム育種の高度化、長期宇宙滞在における宇宙放射線リスク評価において重要な課題である。重イオンマイクロビームを用いた細胞への照準照射はこの課題を解決する上で極めて有効な手法であるが、従来の汎用マイクロビーム照射容器を用いた照射では実現可能な実験が限定されていた。本研究で確立した、ラピッドプロトタイピング技術を用いた、照射実験の要件ごとに形状を最適化したテーラーメード照射容器を製作する技術は、重イオンマイクロビームを用いて実現できる実験の幅を大きく拡大し、重イオン特有の生物効果の解明や、その応用の高度化に寄与する。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (17 results)

All 2019 2018 2017 2016

All Journal Article (10 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Invited: 3 results)

  • [Journal Article] 重イオンマイクロビームを用いた生物照射技術とその利用研究2019

    • Author(s)
      舟山 知夫, 鈴木 芳代
    • Journal Title

      放射線

      Volume: 44 Pages: 151-156

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 放射光マイクロビームを使った生物照射効果研究2019

    • Author(s)
      鈴木 雅雄, 宇佐美 徳子, 舟山 知夫, 横田 裕一郎, 鈴木 芳代, 小林 泰彦
    • Journal Title

      放射線

      Volume: 44 Pages: 147-149

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Validation of Ion Species and Beam Size Availability in Collimating Ion Microbeam System of TIARA2018

    • Author(s)
      Tomoo Funayama, Michiyo Suzuki
    • Journal Title

      QST-M-16, QST Takasaki Annual Report 2017

      Volume: - Pages: 78-78

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Target Irradiation of Individual Cells Using Focusing Heavy-ion Microbeam of QST-Takasaki (VII): Utilization of Polypropylene Film Dish for Analyzing Heavy-ion Hit Effect of Irradiated Cells2017

    • Author(s)
      Funayama Tomoo、Tabei Suguru
    • Journal Title

      QST-M-8, QST Takasaki Annual Report 2016

      Volume: - Pages: 84-84

    • Related Report
      2017 Research-status Report
  • [Journal Article] Histone Deacetylase Inhibitors Sensitize Murine B16F10 Melanoma Cells to Carbon Ion Irradiation by Inducing G1 Phase Arrest2017

    • Author(s)
      Saito Katsuyo、Funayama Tomoo、Yokota Yuichiro、Murakami Takashi、Kobayashi Yasuhiko
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 40 Issue: 6 Pages: 844-851

    • DOI

      10.1248/bpb.b16-01025

    • NAID

      130005688082

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Abscopal Activation of Microglia in Embryonic Fish Brain Following Targeted Irradiation with Heavy-Ion Microbeam.2017

    • Author(s)
      Yasuda T, Kamahori M, Nagata K, Watanabe-Asaka T, Suzuki M, Funayama T, Mitani H, Oda S.
    • Journal Title

      Int J Mol Sci.

      Volume: 18(7) Issue: 7 Pages: 1428-1428

    • DOI

      10.3390/ijms18071428

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 量研機構・高崎の重イオンマイクロビーム2016

    • Author(s)
      舟山 知夫, 横田 裕一郎, 鈴木 芳代
    • Journal Title

      放射線生物研究

      Volume: 51 Pages: 336-356

    • NAID

      40021064788

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 重イオン線によるバイスタンダー応答の特徴2016

    • Author(s)
      冨田 雅典, 松本 英樹 , 舟山 知夫
    • Journal Title

      放射線生物研究

      Volume: 51 Pages: 324-335

    • NAID

      40021064759

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] In vivo 3D analysis of systemic effects after local heavy-ion beam irradiation in an animal model2016

    • Author(s)
      Nagata K, Hashimoto C, Watanabe-Asaka T, Itoh K, Yasuda T, Ohta K, Oonishi H, Igarashi K, Suzuki M, Funayama T, Kobayashi Y, Nishimaki T, Katsumura T, Oota H, Ogawa M, Oga A, Ikemoto K, Itoh H, Kutsuna N, Oda S, and Mitani H
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 28691-28691

    • DOI

      10.1038/srep28691

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of Live-Cell Imaging System for Long-Term Analysis of Bystander Cell Populations Irradiated with Heavy-Ion Microbeams2016

    • Author(s)
      T. Funayama, Y. Yokota , Y. Kobayashi
    • Journal Title

      QST-M-2, QST Takasaki Annual Report 2015

      Volume: - Pages: 109-109

    • Related Report
      2016 Research-status Report
    • Open Access
  • [Presentation] 放射線の微視的線量分布とマイクロビーム2018

    • Author(s)
      舟山 知夫
    • Organizer
      日本放射線影響学会第61回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 重イオンマイクロビームを用いた生命科学研究2018

    • Author(s)
      舟山 知夫, 鈴木 芳代
    • Organizer
      量子生命科学研究会第2回学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 重イオンマイクロビームを用いた生物照射技術とその利用研究2018

    • Author(s)
      舟山 知夫、鈴木 芳代
    • Organizer
      第65回応用物理学会春季学術講演会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 重イオンマイクロビームシステムオフライン観察系の高度化2017

    • Author(s)
      舟山 知夫, 横田 裕一郎
    • Organizer
      第1回QST高崎研シンポジウム
    • Place of Presentation
      高崎量子応用研究所(群馬県・高崎市)
    • Year and Date
      2017-01-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 量研高崎の重イオンマイクロビームを用いた生物照射効果解析技術2017

    • Author(s)
      舟山 知夫、鈴木 芳代、横田 裕一郎
    • Organizer
      日本宇宙生物科学会第31回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 量研高崎におけるマイクロビーム生物研究2017

    • Author(s)
      舟山 知夫、鈴木 芳代、横田 裕一郎
    • Organizer
      日本放射線影響学会第60回大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 重イオンマイクロビームを用いたモデル生物研究2016

    • Author(s)
      舟山 知夫, 白井 孝治, 保田 隆子, 浅香 智美, 尾田 正二, 三谷 啓志, 鈴木 芳代
    • Organizer
      日本放射線影響学会第59回大会
    • Place of Presentation
      JMSアステールプラザ(広島県・広島市)
    • Year and Date
      2016-10-26
    • Related Report
      2016 Research-status Report
    • Invited

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi