| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Genome editing, such as TALEN and CRISPR/Cas9, are powerful tools that can be applied to species that have traditionally been difficult to genetic modification. In this study, we attempted to construct a genome editing system that can recycle selection marker and aimed to develop a technology to modify multiple genes in marine diatoms. Through this study, we developed episome-type genome-editing plasmids for diatoms, which allowed for the introduction and retention into diatom cells, as well as plasmid curing and reintroduction. It was also demonstrated that the developed technology can be applied to the modification of various genes. Therefore, this study completes the construction of a multiple genome editing system in marine diatoms.
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