Dietary protein reduces hepatic steatosis and prevents non-alcoholic fatty liver disease

• Project/Area Number: 16K00849
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Kanazawa University
• Principal Investigator: Watanabe Hitoshi 金沢大学, 新学術創成研究機構, 特任助教 (50721495)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 脂肪肝 / NAFLD / 食品 / タンパク質 / 肝臓 / 蛋白質 / 栄養学

Outline of Final Research Achievements

The prevalence of NAFLD has increased with the increased prevalence of obesity and metabolic syndrome. Recent studies indicate that NAFLD onset and progression may be influenced by the nutrients in food as well as the total calories ingested. However, it isn’t revealed whether protein in food prevents NAFLD progression. In this study, we evaluated the potential of mung bean protein isolate (MPI) to prevent NAFLD progression. MPI decreased the hepatic triglyceride concentration by inhibition of fatty acid synthesis. Additionally, NAFLD progression by atherogenic diet was prevented by MPI intake. These results indicate that MPI reduced hepatic triglyceride accumulation and may be a food to prevent NAFLD progression.

Academic Significance and Societal Importance of the Research Achievements

肥満・インスリン抵抗性の増加に伴い、NAFL・NASHともに罹患率が増加している。一方で、NAFLDの予防・治療は、食事療法以外には確立されていない。本研究において、食品中タンパク質が肝臓脂肪蓄積量とNAFLDの予防・治療に有用であるかを解明することは、摂取タンパク質の「質」に依存した新規食事療法を生み出すことであり、食事療法のメカニズムの解明や抗NAFLD食事療法の効率化に繋がると考えられる。

Research Products

• [Journal Article] Dietary mung bean protein reduces high-fat diet-induced weight gain by modulating host bile acid metabolism in a gut microbiota-dependent manner. (2018)
  • Author(s): Nakatani A, Li X, Miyamoto J, Igarashi J, Watanabe H, Sutou A, Watanabe K, Motoyama T, Tachibana N, Kohno M, Inoue H, Kimura I.
  • Journal Title: Biochem Biophys Res Commun. Volume: 501 Issue: 4 Pages: 955-961
  • DOI: 10.1016/j.bbrc.2018.05.090
  • Peer Reviewed / Open Access
• [Journal Article] Sirt2 facilitates hepatic glucose uptake by deacetylating glucokinase regulatory protein (2018)
  • Author(s): Watanabe Hitoshi、Inaba Yuka、Kimura Kumi、Matsumoto Michihiro、Kaneko Shuichi、Kasuga Masato、Inoue Hiroshi
  • Journal Title: Nat Commun. Volume: 9 Issue: 1 Pages: 30-30
  • DOI: 10.1038/s41467-017-02537-6
  • Peer Reviewed / Open Access
• [Journal Article] Dietary Mung Bean Protein Reduces Hepatic Steatosis, Fibrosis, and Inflammation in Male Mice with Diet-Induced, Nonalcoholic Fatty Liver Disease. (2017)
  • Author(s): Watanabe H, Inaba Y, Kimura K, Asahara SI, Kido Y, Matsumoto M, Motoyama T, Tachibana N, Kaneko S, Kohno M, Inoue H*.
  • Journal Title: J Nutr. Volume: 147 Issue: 1 Pages: 52-60
  • DOI: 10.3945/jn.116.231662
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cyclophilin A is a new M cell marker of bovine intestinal epithelium. (2016)
  • Author(s): Tetsuya Hondo , Shunsuke Someya , Yuya Nagasawa , Shunsuke Terada, Hitoshi Watanabe, Xiangning Chen, Kouichi Watanabe, Shyuichi Ohwada, Haruki Kitazawa, Michael T. Rose, Tomonori Nochi, Hisashi Aso
  • Journal Title: Cell and Tissue Research Volume: 364 Issue: 3 Pages: 585-597
  • DOI: 10.1007/s00441-015-2342-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] SIRT2はグルコキナーゼ調節タンパク質の脱アセチル化を介して肝臓糖取り込みを制御する (2018)
  • Author(s): 渡邉一史、稲葉有香、松本道宏、春日雅人、井上啓
  • Organizer: 日本肥満学会
• [Presentation] NAD+は肝糖取り込みを調節する (2018)
  • Author(s): 渡邉一史、稲葉有香、松本道宏、春日雅人、井上啓
  • Organizer: アディポサイエンス・シンポジウム
• [Presentation] SIRT2による肝臓糖取り込み機構の解明 (2018)
  • Author(s): 渡邉一史、松本道宏、春日雅人、井上啓
  • Organizer: 日本内分泌学会学術総会
• [Presentation] 肥満モデルにおける肝糖取込み障害メカニズムの解明 (2018)
  • Author(s): 渡邊一史、松本道宏、春日雅人、井上啓
  • Organizer: 第32回日本糖尿病・肥満動物学会年次学術集会
• [Presentation] NAD+/SIRT2作用の低下は肝糖取り込みを障害する (2017)
  • Author(s): 渡邉一史、春日雅人、井上啓
  • Organizer: 第38回日本肥満学会
• [Presentation] NAD+による肝糖取り込み調節機構の解析 (2017)
  • Author(s): 渡邉一史、稲葉有香、木村久美、松本道宏、金子周一、春日雅人、井上啓
  • Organizer: 第35回内分泌代謝学サマーセミナー
• [Presentation] SIRT2の肝臓糖取り込みにおける重要性 (2016)
  • Author(s): 渡邉一史、松本道宏、春日雅人、井上啓
  • Organizer: 第28回分子糖尿病学シンポジウム
  • Place of Presentation: 富山 タワー111
  • Year and Date: 2016-12-03