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Development of quantification method for allergenic proteins in peach and sweet cherry by targeted proteomics

Research Project

Project/Area Number 16K00939
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Eating habits
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Ippoushi Katsunari  国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門, 上級研究員 (30355606)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsターゲット・プロテオミクス / モモ / オウトウ / アレルゲン / LC/MS/MS / 安定同位体標識内部標準ペプチド / 核果類 / 定量 / アレルゲンタンパク質 / LC-MS/MS / Pru av 1 / Pru av 3 / Pru av 4 / Pru p 1 / Pru p 3 / プロテオミクス
Outline of Final Research Achievements

Pru p 1 and Pru p 3 are the main allergenic proteins of peach. In this study, we developed a quantitative method for these proteins based on protein absolute quantification with LC/MS/MS, and LVASPSGGSIIK* (K*: stable isotope-labelled K) and ISASTNC[CAM]ATVK* (C[CAM]: carbamidomethyl-modified C), internal standard peptides. Using the same methodology used for peach allergens, we developed a quantitative method for Pru av 1, Pru av 3, and Pru av 4, main allergenic proteins contained in sweet cherry.

Academic Significance and Societal Importance of the Research Achievements

本研究課題で開発された定量法は、低・無アレルゲンモモやオウトウ品種の育成および栽培技術の研究開発への利用が期待される。
食品表示法では、モモを原材料として含む加工食品については、モモを原材料として含む旨を可能な限り表示するよう努めることとされている。さらなる研究開発を行うことにより、本定量法はモモのアレルギー食品表示の検証などへの応用が期待される。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Assessment of Pru p 1 and Pru p 3 in peach fruit by liquid chromatography-tandem mass spectrometry2019

    • Author(s)
      Ippoushi Katsunari、Tanaka Yoshimi、Wakagi Manabu、Hashimoto Naoto、Takano-Ishikawa Yuko
    • Journal Title

      Journal of Food Composition and Analysis

      Volume: 80 Pages: 10-15

    • DOI

      10.1016/j.jfca.2019.03.010

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Analysis of cherry allergens by liquid chromatography/tandem mass spectrometry using stable isotope-labelled peptides2019

    • Author(s)
      Katsunari Ippoushi, Yoshimi Tanaka, Manabu Wakagi, Naoto Hashimoto, Yuko Takano-Ishikawa
    • Organizer
      48TH International Symposium on High-Performance Liquid Phase Separations and Related Techniques
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] LC/MS/MSと安定同位体標識ペプチドを用いたモモアレルゲンタンパク質の定量法2018

    • Author(s)
      一法師克成、田中良美、若木学、橋本直人、石川(高野) 祐子
    • Organizer
      日本分析化学会第67年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Quantification method of peach allergens by liquid chromatography/tandem mass spectrometry with the use of stable isotope-labelled peptides2017

    • Author(s)
      Katsunari Ippoushi, Yoshimi Tanaka, Manabu Wakagi, Naoto Hashimoto, Yuko Takano-Ishikawa
    • Organizer
      31st EFFoST International Conference
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2021-02-19  

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