| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The purpose of this study is the establishment of the genome editing technology that easily enables to modify the targeted genomic locus in a model vertebrate zebrafish. Furthermore, this technology was applied to insert efficiently the reporter gene at the targeted genomic locus, leading to monitor its gene expression during embryogenesis.
The biological experiment acceptable for observation of genome modifications is useful to understand properties in genome. In this study, I established the biological experiment that is suitable to consider genome editing efficiency and various genomic mutations induced by genome editing using zebrafish embryos modified by the genome editing technology.
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