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Innate immune responses in the silkworm

Research Project

Project/Area Number 16K01018
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Science education
Research InstitutionNara University of Education

Principal Investigator

MORIMOTO Koichi  奈良教育大学, 理科教育講座, 教授 (70243350)

Research Collaborator SUGIMURA yukio  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords免疫 / カイコ / 高等学校 / 自然免疫 / 科学教育
Outline of Final Research Achievements

Insect immune system is divided into two major reaction types, cellular and humoral immune responses.
Actual protective responses were shown in the silkworm larvae, Bombyx mori, which had be anaesthetized on a frozen thermal gel and subsequently injected with various inocula. As a cellular response, phagocytic activity of hemocytes was observed 3 hr after injection of India ink, followed by accumulation of the ink carbon particles in hemocytes for successive 1 day.Chemical protection induced by humoral immune response was also examined, using larvae injected with heat-killed bacteria and bacterial lipo-polysaccharide (LPS). Antibacterial activities in hemolymph were detected constantly 6-24 hr after injection, which were assayed by the agar cup-plate method. Based on the present study, the topic of insect immune responses is thought to be accepted as a useful teaching material in high school.

Academic Significance and Societal Importance of the Research Achievements

カイコ卵や幼虫はインターネットを通じて購入でき、人工飼料でも飼育できるため、周年に亘り飼育できる。また、カイコ5齢幼虫は大きいので(体長7cm位),取り扱いが容易である.また, 1頭から400 μL体液が採取できるので、数名の生徒で共有して,実験を進めることができ,免疫実験の昆虫として都合がよい。本研究を基に,細胞性防御応答である食作用の観察と液性の化学的防御応答である抗菌タンパク質の検出を統合した実験を提案した.

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (3 results)

All 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] カイコの自然免疫2017

    • Author(s)
      杉村順夫、森本弘一、尾山廣
    • Journal Title

      生物教育

      Volume: 59 Pages: 10-18

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] カイコに対する水中麻酔法の教材化2018

    • Author(s)
      森本弘一、杉村順夫
    • Organizer
      日本生物教育学会
    • Related Report
      2017 Research-status Report
  • [Presentation] カイコの生活環に関する教材化2017

    • Author(s)
      森本弘一、杉村順夫
    • Organizer
      日本生物教育学会
    • Place of Presentation
      東京学芸大学
    • Year and Date
      2017-01-07
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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