| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
Calcium sensitive dye Fluo4 was successfully perfused into the isolated rat heart in ex-vivo condition.
MALDI imaging was performed to construct a 3D map of TRPM4 protein in the heart, which allowed simultaneous mapping of more than 57 proteins at 100 μm resolution.
We developed a new experiment system using human iPS cardiomyocytes and models of ischemia-reperfusion injury, based on the previous system using rat heart and cardiomyocytes. Pretreatment of an inhibitor of TRPM4 channel protected the iPS cardiomyocytes from cell injury under ischemia-reperfusion condition. This result suggests that TRPM4 channel is involved in ischemia-reperfusion injury in human as well.
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