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Elucidation of the mechanism of arrhythmia in cardiac ischemia-reperfusion injury by imaging technology

Research Project

Project/Area Number 16K01356
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionOkayama University

Principal Investigator

Takahashi Ken  岡山大学, 医歯薬学総合研究科, 助教 (50432258)

Research Collaborator Naruse Keiji  
Wang Chen  
Miyaji Takaaki  
Wei Heng  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords心筋梗塞 / 虚血再灌流障害 / MALDI-TOF / TRPM4 / CRISPR/Cas9 / ミトコンドリア膜電位 / 細胞内ATP濃度 / iPS細胞 / TRPM4チャネル / ラット心筋細胞株H9c2 / TRPM2 / 細胞・組織 / 循環器・高血圧 / 生理学 / 生体分子
Outline of Final Research Achievements

Calcium sensitive dye Fluo4 was successfully perfused into the isolated rat heart in ex-vivo condition.
MALDI imaging was performed to construct a 3D map of TRPM4 protein in the heart, which allowed simultaneous mapping of more than 57 proteins at 100 μm resolution.
We developed a new experiment system using human iPS cardiomyocytes and models of ischemia-reperfusion injury, based on the previous system using rat heart and cardiomyocytes. Pretreatment of an inhibitor of TRPM4 channel protected the iPS cardiomyocytes from cell injury under ischemia-reperfusion condition. This result suggests that TRPM4 channel is involved in ischemia-reperfusion injury in human as well.

Academic Significance and Societal Importance of the Research Achievements

心機能に関し、ヒトとラット・マウスとの間の違いが従来より指摘されている。従って、本研究でヒト由来のiPS心筋細胞を用いる心臓虚血再灌流障害の実験系を確立した意義は極めて大きい。本研究をさらに進めることにより、ヒトの心筋梗塞の予防法開発につながることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017 Other

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Role of the TRPM4 Channel in Cardiovascular Physiology and Pathophysiology2018

    • Author(s)
      Wang C, Naruse K, Takahashi K
    • Journal Title

      Cells

      Volume: 7(6) Issue: 6 Pages: 62-62

    • DOI

      10.3390/cells7060062

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of Oxidative Stress on Cardiovascular System in Response to Gravity2017

    • Author(s)
      Takahashi Ken, Okumura Hiroki, Guo Rui, Naruse Keiji
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 18 Issue: 7 Pages: 1426-1426

    • DOI

      10.3390/ijms18071426

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Mechanobiology and its application to medical problem solving2019

    • Author(s)
      Takahashi K
    • Organizer
      2019 International Conference on Biology and Medical Sciences
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cellular model of ischemic heart disease using human induced pluripotent stem cells2018

    • Author(s)
      Wei H, Wang C, Takahashi K, Naruse K
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] TRPM4 channel is involved in cellular damage caused by simulated ischemia-reperfusion injury: study of human iPSC-derived cardiomyocytes2018

    • Author(s)
      Wang C, Wei H, Naruse K, Takahashi K
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] TRPM4 channel is involved in cellular damage caused by simulated ischemia-reperfusion injury: study of human iPSC-derived cardiomyocytes2018

    • Author(s)
      Wang C, Wei H, Naruse K, Takahashi K
    • Organizer
      The 95th Annual Meeting of the Physiological Society of Japan
    • Related Report
      2017 Research-status Report
  • [Presentation] Cellular model of ischemic heart disease using human induced pluripotent stem cells2018

    • Author(s)
      Wei H, Wang C, Takahashi K, Naruse K
    • Organizer
      The 95th Annual Meeting of the Physiological Society of Japan
    • Related Report
      2017 Research-status Report
  • [Presentation] Effect of dimensional factor of cell culture on cardiac differentiation of human induced pluripotent stem cells2018

    • Author(s)
      Tada C, Takahashi K, Naruse K
    • Organizer
      The 95th Annual Meeting of the Physiological Society of Japan
    • Related Report
      2017 Research-status Report
  • [Presentation] Simultaneous mapping of multiple proteins in heart using matrix-assisted laser desorption/ionization imaging mass spectrometry2017

    • Author(s)
      Takahashi K, Naruse K
    • Organizer
      European Society of Cardiology Annual Congress 2017
    • Place of Presentation
      Barcelona
    • Year and Date
      2017-08-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒトiPS細胞を用いた虚血性心疾患モデルの構築2017

    • Author(s)
      魏恒,王晨,高橋賢,成瀬恵治
    • Organizer
      第69回日本生理学会中国・四国地方会
    • Related Report
      2017 Research-status Report
  • [Presentation] Simultaneous mapping of multiple proteins in heart using matrix-assisted laser desorption/ionization imaging mass spectrometry2017

    • Author(s)
      Takahashi K, Naruse K
    • Organizer
      European Society of Cardiology Annual Conference 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Remarks] 高橋賢の研究Blog

    • URL

      https://ken-takahashi.net/?cat=4

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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