| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Nanosecond high-voltage electric pulses (NHEPs) are recently regarded as a novel physical method for efficient induction of cell death. In this study, mechanistic investigations of the biological actions of NHEPs demonstrated that NHEPs induce gross crosslinking of intracellular proteins, which accounts for a part of the cytotoxicity of NHEPs. Next, cancer cell spheroids were used as an in vitro model for cancer therapy with NHEPs. A novel electric device was employed to generate NHEPs with various pulse widths. NSEP-exposed spheroids were promptly disintegrated and exhibited a significant reduction in viability. Taken together, this study provided mechanistic insights into NHEP actions and indicated the usefulness of NHEPs in cancer treatment.
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