| Project/Area Number |
16K01374
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Shibaura Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 細胞培養 / バイオセンサ / 抗菌ペプチド / 等速電気泳動 / アプタマー / ケラチノサイト / ３次元皮膚構造 / 細胞培養デバイス / バリア性 / 共培養 / オンチップインキュベーション / 培養表皮 |
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| Outline of Final Research Achievements |
Cell culture is vulnerable to microbial contamination, which is one of the reasons that cell culture is less portable. We focused on the possibility that the antimicrobial peptides released by keratinocytes that are introduced into the device could make cell culture robust against microbial contamination. To verify the effect, it is necessary to detect antimicrobial peptides with high sensitivity in a small amount of culture media sampled continuously. We developed a system that can detect the antimicrobial peptide LL-37 contained in a very small amount of medium of about 20 microliters with high sensitivity by selective LL-37 complex formation with an LL-37 aptamer, and separation of the complexes using isotachophoresis.
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| Academic Significance and Societal Importance of the Research Achievements |
原因未同定あるいは潜在的な健康問題・環境上の脅威を検出できる「細胞ベース」バイオセンサ，または，細胞を移植する医療（細胞医療）など，研究対象としての培養細胞を，医療・環境等の分野で広く応用するための技術に寄与できる．
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