| Project/Area Number |
16K01381
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Yokohama College of Pharmacy |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
岩瀬 由未子 横浜薬科大学, 薬学部, 講師 (00521882)
梅村 晋一郎 東北大学, 医工学研究科, 学術研究員 (20402787)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 超音波 / 薬剤修飾カーボンナノチューブ / ナノ粒子 / 音響化学活性 / 音響化学療法 / ＤＤＳ / カーボンナノチューブ |
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| Outline of Final Research Achievements |
Poly ethylene glycol -modified carbon nanotubes (PEG-modified CNTs) have attracted particular attention due to their potential application to tumor imaging and therapy as carbon nano materials. In this study, the sonodynamically induced antitumor effect of PHF was investigated. Both in vitro and in vivo antitumor effects were tested in combination with ultrasound at 2 MHz. The rate of ultrasonically induced damage on isolated sarcoma180 cells in air-saturated suspension was enhanced by twice with 80 microM PHF. This enhancement was significantly inhibited by histidine, which may suggest it was mediated by ultrasonically induced oxidation. The coadministraion of 25 mg/kg PEG-modified CNTs followed by ultrasonic exposure at 2 MHz suppressed the growth of implanted colon 26 tumors at an intensity with which ultrasound alone showed only a slight antitumor effect. The induction of apoptosis observed after the combination treatment of PHF with ultrasound.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は超音波とPEG-modified CNTsなどの音響化学物質を併用することにより腫瘍を選択的に単離または固形腫瘍細胞に対する抗腫瘍作用を確認することができた。これは、副作用が少なく腫瘍およびその周辺部位に対し選択的治療効果を示す治療法の開発に役立つものと考えられる。
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