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Development of soft nanoparticle composite materials based on decellularized tissues and its application to treatment of myocardial infarction

Research Project

Project/Area Number 16K01387
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTokyo Medical and Dental University

Principal Investigator

HASHIMOTO YOSHIHIDE  東京医科歯科大学, 生体材料工学研究所, 助教 (40638384)

Co-Investigator(Kenkyū-buntansha) 根岸 淳  信州大学, 繊維学部, 助教(特定雇用) (60722634)
Research Collaborator Funamoto Seiichi  
Yamashita Akitatsu  
Akiyoshi Kazunari  
Project Period (FY) 2016-10-21 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsナノゲル / 脱細胞化生体組織 / ECMハイドロゲル / ECMゲル / 心筋リモデリング / 心筋梗塞 / 高静水圧 / 脱細胞化 / マイクロ加工 / 心筋修復 / 脱細胞化組織 / タンパク質 / 心筋梗塞治療
Outline of Final Research Achievements

we have developed decellularized porcine myocardium, bladder matrix (UBM), and small intestinal submucosa (SIS) by surfactant method and high-hydrostatic pressure (HHP) method. The tissue structure of decellularized tissue prepared by HHP method was maintained compared with surfactant method.
We have investigated the efficacy of nanogel-crosslinked gel or nanogel-crosslinked gel/UBM-ECM gel composite material using rat myocardial infarction model. As a result, it revealed that nanogel-crosslinked gel/UBM-ECM gel suppressed the myocardial thinning and fibrosis.

Academic Significance and Societal Importance of the Research Achievements

本研究で提案する脱細胞化組織-ナノゲル複合材料は、脱細胞化組織に存在する増殖因子などの薬物をナノゲルが取り込み、徐放するDDS機能と細胞の三次元足場として働く機能を有する。この材料は、心筋梗塞で心機能が低下した多くの患者のQOLの改善に役立つ可能性が期待できる。また、様々な組織の再生に応用可能であるばかりか、種々の幹細胞の機能制御に新たなツールを与え、iPS 細胞などを用いた再生医療の発展に貢献することが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (22 results)

All 2019 2018 2017

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (15 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results)

  • [Journal Article] A fibrin‐coated pericardial extracellular matrix prevented heart adhesion in a rat model2018

    • Author(s)
      Funamoto Seiichi、Hashimoto Yoshihide、Kishida Akio、Negishi Jun
    • Journal Title

      Journal of Biomedical Materials Research Part B: Applied Biomaterials

      Volume: 107 Issue: 4 Pages: 1088-1094

    • DOI

      10.1002/jbm.b.34201

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 開心術後の癒着防止材の評価を目的とした心臓癒着動物モデルの構築および製品評価2018

    • Author(s)
      山下暁立, 舩本誠一, 張永巍, 橋本良秀, 岸田晶夫
    • Journal Title

      胸部外科

      Volume: 71 Pages: 658-663

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nanogel tectonics for tissue engineering: Protein delivery systems with nanogel chaperones2018

    • Author(s)
      Yoshihide Hashimoto, Sada-atsu Mukai, Yoshihiro Sasaki, Kazunari Akiyoshi
    • Journal Title

      Adv Healthc Mater

      Volume: 7 Issue: 23

    • DOI

      10.1002/adhm.201800729

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Water absorption by decellularized dermis2018

    • Author(s)
      Zhang Yongwei、Iwata Takuya、Nam Kwangwoo、Kimura Tsuyoshi、Wu Pingli、Nakamura Naoko、Hashimoto Yoshihide、Kishida Akio
    • Journal Title

      Heliyon

      Volume: 4 Issue: 4 Pages: e00600-e00600

    • DOI

      10.1016/j.heliyon.2018.e00600

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of small-diameter vascular grafts reconstructed from decellularized aorta sheets2017

    • Author(s)
      J. Negishi, Y. Hashimoto, A. Yamashita, Y. Zhang, T. Kimura, A. Kishida, S. Funamoto
    • Journal Title

      J Biomed Mater Res Part A

      Volume: 105 Issue: 5 Pages: 1293-1298

    • DOI

      10.1002/jbm.a.36017

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Histological structure affects recellularization of decellularized arteries2017

    • Author(s)
      J. Negishi, Y. Hashimoto, A. Yamashita, T. Kimura, A. Kishida, S. Funamoto
    • Journal Title

      Mater Sci Eng C-Mater Biol Appl

      Volume: 70 Pages: 450-455

    • DOI

      10.1016/j.msec.2016.09.004

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nanogel-integrated pH responsive composite hydrogels for controlled drug delivery2017

    • Author(s)
      G. E. Cinay, M. Alipour, Y. Hashimoto, Y. Sasaki, K. Akiyoshi, S. Kizilel
    • Journal Title

      ACS Biomater Sci Eng

      Volume: 3 Issue: 3 Pages: 370-380

    • DOI

      10.1021/acsbiomaterials.6b00670

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 種々の脱細胞化ECMゲルを用いた血管網構築2019

    • Author(s)
      木村剛, 門田純平, 橋本良秀, 藤里俊哉, 岸田晶夫
    • Organizer
      第18回日本再生医療学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ウシ血管を用いた脱細胞化小口径動脈グラフトの作製2019

    • Author(s)
      橋本良秀, 山下暁立, 張永巍, 舩本誠一, 岸田晶夫
    • Organizer
      第21 回日本異種移植研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 組織再生プラットフォームとしての脱細胞化生体組織とその周辺技術2019

    • Author(s)
      橋本良秀
    • Organizer
      医工連携セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 血管網構築を目指した脱細胞化ECMゲルの開発2019

    • Author(s)
      門田純平, 橋本良秀, 藤里俊哉, 木村剛, 岸田晶夫
    • Organizer
      第28回インテリジェント・ナノ材料シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Characterization of ECM hydrogels prepared by different decellularization methods2018

    • Author(s)
      Junpei Kadota, Yoshihide Hashimoto, Toshiya Fujisato, Tsuyoshi Kimura, Akio Kishida
    • Organizer
      1st G’L’owing Polymer Symposium in KANTO
    • Related Report
      2018 Annual Research Report
  • [Presentation] フィブリン糊被覆脱細胞化心膜による心臓-胸壁間の癒着防止2018

    • Author(s)
      根岸淳, 橋本良秀, 張 永巍, 山下暁立, 舩本誠一, 岸田晶夫
    • Organizer
      第40回日本バイオマテリアル学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脱細胞化小口径動脈グラフトの作製とin vivo評価2018

    • Author(s)
      橋本良秀, 山下暁立, 張永巍, 舩本誠一, 岸田晶夫
    • Organizer
      第40回日本バイオマテリアル学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 異なる脱細胞化方法で調製したECMヒドロゲルの特性評価2018

    • Author(s)
      門田純平, 橋本良秀, 藤里俊哉, 木村 剛, 岸田晶夫
    • Organizer
      第40回日本バイオマテリアル学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Preparation and characterization of ECM hydrogels derived from acellular urinary bladder matrix (UBM) and small intestinal submucosa (SIS)2018

    • Author(s)
      Junpei Kadota, Yoshihide Hashimoto, Tsuyoshi Kimura, Akio Kishida
    • Organizer
      3rd International Sympodium on Creation of Life Innovation Materials for Interdiciplinary and International Researcher Development (iLIM-3)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In vitro microvascular network formation in/on hydrogels derived from decellularized tissues2018

    • Author(s)
      Junpei Kadota, Yoshihide Hashimoto, Tsuyoshi Kimura, Akio Kishida
    • Organizer
      Frontier 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Preparation and characterization of hydrogels derived fromdecellularized urinary bladder matrix (UBM) and small intestinal submucosa (SIS)2018

    • Author(s)
      Junpei Kadota, Yoshihide Hashimoto, Tsuyoshi Kimura, Akio Kishida
    • Organizer
      5Th TERMIS World Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 人工角膜の最前線2018

    • Author(s)
      橋本良秀
    • Organizer
      2018年度 サイエンスカフェ(最新医歯学研究)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 角膜上皮の創傷治癒に伴う脱細胞化角膜実質のリモデリング2018

    • Author(s)
      橋本良秀, 舩本誠一, 佐々木秀次, 根岸淳, 木村剛, 小林尚俊, 岸田晶夫
    • Organizer
      第27回ライフサポート学会フロンティア講演会
    • Related Report
      2017 Research-status Report
  • [Presentation] 多分岐PEG修飾脱細胞化心膜の開発と癒着防止材への応用2017

    • Author(s)
      橋本良秀 山下暁立 根岸淳,張 永巍 木村剛, 舩本誠一 岸田晶夫
    • Organizer
      第39回日本バイオマテリアル学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ソフトナノ粒子の集積化による再生医療用材料の開発2017

    • Author(s)
      橋本良秀
    • Organizer
      日本真空学会 機能薄膜部会 ナノ構造機能創成専門部会 第8回研究会
    • Related Report
      2017 Research-status Report
    • Invited

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Published: 2016-10-24   Modified: 2020-03-30  

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