Dual release of stromal cell-derived factor-1 and basic fibroblast growth factor with a nerve conduit accelerates peripheral nerve regeneration
| Project/Area Number |
16K01393
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Osaka City University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
上村 卓也 大阪市立大学, 大学院医学研究科, 客員研究員 (10597321)
高松 聖仁 大阪市立大学, 大学院医学研究科, 客員准教授 (30295688)
岡田 充弘 大阪市立大学, 大学院医学研究科, 講師 (40309571)
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| Research Collaborator |
YOKOI Takuya
SHINTANI Kosuke
ONODE Ema
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 末梢神経 / 再生医療 / 人工神経 / iPS細胞 / ストローマ細胞由来因子 / SDF-1 / 線維芽細胞増殖因子 / bFGF / ドラッグデリバリーシステム / 成長因子 / ドラッグデリバリーシステム（DDS） / FGF / SDF |
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| Outline of Final Research Achievements |
In this study, we investigated the efficacy of a nerve conduit coated with stromal cell-derived factor-1 (SDF-1) and basic fibroblast growth factor (bFGF) for peripheral nerve regeneration. We prepared a nerve conduit containning SDF-1 and bFGF co-sustained-release gelatin, and verified the effects in a mouse sciatic nerve defect model. Co-sustained release of SDF-1 and bFGF in the nerve conduit promoted lower limb function recovery and axonal regeneration. A large number of CD34 positive cells and microvessels were found in the nerve conduit, and TGFβ gene expression was elevated early after transplantation. This results suggest that co-sustained release of multiple factors promotes peripheral nerve regeneration with synergy or additive action.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究によって人工神経を足場としたSDF-1とFGFの二重徐放（Dual-release）DDSを構築し、内因性の神経再生促進能を増幅させることで、外的に細胞移植を行わずとも神経再生を促進することが可能であった。この内因性の神経再生力を増幅した治療方法が確立されれば、我々がこれまでに開発した従来の細胞移植（iPS細胞由来神経前駆細胞移植）と組み合わせることによって、神経再生が更に加速した理想的な再生医療が可能になり、現在の標準的治療である自家神経移植に伴う欠点を克服することが可能になり、その社会的意義は大きい。
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Report
(4 results)
Research Products
(64 results)
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[Journal Article] Validity and Reproducibility of Various Measurement Methods for Craniocervical Sagittal Balance.2018
Author(s)
Tamai K, Suzuki A, Takahashi S, Hoshino M, Toyoda H, Hayashi K, Ohyama S, Kishimura Y, Onode E, Umano M, Terai H, Nakamura H.
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Journal Title
Clin Spine Surg.
Volume: 31(2)
Issue: 2
Pages: 80-85
DOI
Related Report
Peer Reviewed
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[Journal Article] Differences in short-term clinical and radiological outcomes depending on timing of balloon kyphoplasty for painful osteoporotic vertebral fracture.2018
Author(s)
Takahashi S, Hoshino M, Terai H, Toyoda H, Suzuki A, Tamai K, Watanabe K, Tsujio T, Yasuda H, Kono H, Sasaoka R, Dohzono S, Hayashi K, Ohyama S, Hori Y, Nakamura H.
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Journal Title
J Orthop Sci.
Volume: 23(1)
Issue: 1
Pages: 51-56
DOI
Related Report
Peer Reviewed
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[Journal Article] Characterizing the course of back pain after osteoporotic vertebral fracture: a hierarchical cluster analysis of a prospective cohort study.2017
Author(s)
Toyoda H, Takahashi S, Hoshino M, Takayama K, Iseki K, Sasaoka R, Tsujio T, Yasuda H, Sasaki T, Kanematsu F, Kono H, Nakamura H.
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Journal Title
Arch Osteoporos.
Volume: 12(1)
Issue: 1
Pages: 82-82
DOI
Related Report
Peer Reviewed
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[Book] 整形外科2018
Author(s)
上村卓也、高松聖仁、中村博亮
Total Pages
1
Publisher
南光堂
Related Report
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[Book] 人工臓器2018
Author(s)
横井卓哉、上村卓也、高松聖仁、中村博亮
Total Pages
4
Publisher
日本人工臓器学会
Related Report
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