| Project/Area Number |
16K01437
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical engineering assessment
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| Research Institution | Osaka University |
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Principal Investigator |
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| Research Collaborator |
Maki Ayaka
Lin Ying
Sato Kenta
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | DNAメチル化 / ナノ銀粒子 / プロテアソーム / ナノマテリアル / エピジェネティクス |
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| Outline of Final Research Achievements |
In this research, based on the new viewpoint of "epigenetic control by nanomaterial", we tried to analyze the nanomaterial-induced biological responses by analyzing the relationship between physical property-biological responses-epigenetic control. As a result, silver nanoparticles, which are used for things familiar to our lives, 1) decreased DNA methylation rate for human alveolar epithelial cell lines, and reduced a protein amount of DNA methylation enzyme, Dnmt1. In addition, 2) the decrease in the amount of Dnmt1 was resulted from proteasomal degradation by promoting ubiquitination of Dnmt1. Investigating the biological responses through epigenetic control can contribute to the identification of new biological responses induced by nanomaterials and the elucidation of its mechanism.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、エピジェネティクス制御をも考慮に入れた、ナノマテリアルの安全性評価手法やハザード回避手法の開発へと展開できるものと期待される。また、ナノマテリアルによるハザード発現機構の解明は、有用かつ安全なナノ医薬品の開発支援に資する基盤情報を提供することで、創薬プロセスの効率化に直結し、我が国のナノ医薬品開発を大きく推進させる原動力となる。従って本研究成果は、ナノ安全科学研究とも言うべきものであり、安全な薬物治療による疾病克服を実現し、国民に健康で安全な社会を提供すると共に、ナノ医薬品産業の国際競争力の強化をもたらすと予想される。
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