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The role of satellite cells in skeletal muscle function -Preparation and investigation of the chimeric multinuclear myotubes-

Research Project

Project/Area Number 16K01727
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Sports science
Research InstitutionThe University of Shiga Prefecture

Principal Investigator

Nakai Naoya  滋賀県立大学, 人間文化学部, 教授 (90324508)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords筋サテライト細胞 / 骨格筋 / 糖代謝 / タンパク質合成 / 分岐鎖アミノ酸 / オートファジー / アミノ酸 / サテライト細胞 / ロイシン / メカニカルストレス
Outline of Final Research Achievements

Skeletal muscle satellite cell is a stem cell, which located in a niche on the surface of the muscle fiber. We examined the function of satellite cell in vitro. Whereas the acquired environmental factors such as abnormality of glucose metabolism depressed the function of skeletal muscle, the isolated satellite cells retained its normal function in vitro. The isolated gene knock-out satellite cells exhibited the same characteristics as gene knock-out skeletal muscle. In addition, multinuclear myotubes which formed by the mixture of gene knock-out satellite cells and wild-type satellite cells (chimeric myotube), exhibited the intermediate characteristics. Thus, nuclei from wild-type satellite cell could not fully compensate the function of the nuclei from gene knock-out satellite cell, even both nuclei were located in the same myotube.

Academic Significance and Societal Importance of the Research Achievements

機能が低下した骨格筋から採取した筋サテライト細胞を適正な条件で培養し、筋分化させるとほぼ正常な機能を回復できる可能性を示した点で、本研究の社会的意義がある。すなわち、生活習慣を見直し、正常な代謝に回復できれば以前の生理機能を取り戻せる可能性を示した。また、骨格筋細胞が多核細胞であることを利用して、遺伝的背景の異なる筋サテライト細胞からキメラ筋細胞を作成し、正常な核による補償効果を解析した点に学術的意義があるが、結果からは両者の核が同じ細胞内に存在しても遺伝子欠損の影響は補償できないことが明らかになった。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2019 2018 2017 2016 Other

All Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (4 results) Remarks (2 results)

  • [Journal Article] Low-carbohydrate high-protein diet diminishes the insulin response to glucose load via suppression of SGLT-1 in mice.2019

    • Author(s)
      Higashida K, Terada S, Li X, Inoue S, Iida N, Kitai S, Nakai N.
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 82 Issue: 2 Pages: 365-371

    • DOI

      10.1080/09168451.2018.1533803

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Leucine supplementation after mechanical stimulation activates protein synthesis via L-type amino acid transporter 1 in vitro.2018

    • Author(s)
      Nakai N, Kawano F, Murakami T, Nakata K, Higashida K.
    • Journal Title

      J Cell Biochem.

      Volume: 119(2) Issue: 2 Pages: 2094-2101

    • DOI

      10.1002/jcb.26371

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Vibration acceleration promotes bone formation in rodent models2017

    • Author(s)
      Uchida, R., Nakata, K., Kawano, F., Yonetani, Y., Ogasawara, I., Nakai, N., Mae, T., Matsuo, T., Tachibana, Y., Yokoi, H., Yoshikawa, H.
    • Journal Title

      PLoS One

      Volume: 12 Issue: 3 Pages: e0172614-e0172614

    • DOI

      10.1371/journal.pone.0172614

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Prenatal myonuclei play a crucial role for skeletal muscle hypertrophy in rodents.2017

    • Author(s)
      Kawano F, Ono Y, Fujita R, Watanabe A, Masuzawa R, Shibata K, Hasegawa S, Nakata K, Nakai N
    • Journal Title

      American Journal of Physiology Cell Physiology

      Volume: 312 Issue: 3 Pages: C233-C243

    • DOI

      10.1152/ajpcell.00151.2016

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Muscle-specific deletion of BDK amplifies loss of myofibrillar protein during protein undernutrition.2017

    • Author(s)
      Ishikawa T, Kitaura Y, Kadota Y, Morishita Y, Ota M, Yamanaka F, Xu M, Ikawa M, Inoue N, Kawano F, Nakai N, Murakami T, Miura S, Hatazawa Y, Kamei Y, Shimomura Y.
    • Journal Title

      Sci Rep.

      Volume: 7 Issue: 1 Pages: 39825-39825

    • DOI

      10.1038/srep39825

    • NAID

      120006957417

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] グルコース枯渇はC2C12細胞のタンパク質合成シグナルの低下およびオートファジーを誘導する2018

    • Author(s)
      北井彩貴、飯田典子、井上祥花、東田一彦、中井直也
    • Organizer
      日本栄養・食糧学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 分岐鎖アミノ酸代謝の亢進が筋サテライト細胞のタンパク質合成に及ぼす影響2018

    • Author(s)
      中井直也、飯田典子、北井彩貴、井上祥花、北浦靖之、下村吉治、東田一彦
    • Organizer
      日本体力医学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 短期間の糖代謝異常は筋サテライト細胞のインスリンシグナルを低下させる2017

    • Author(s)
      成澤諒子、東田一彦、中井直也
    • Organizer
      日本体力医学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 筋サテライト細胞を用いた培養骨格筋細胞モデルによるタンパク質合成促進作用機序の解析2016

    • Author(s)
      中井直也、東田一彦
    • Organizer
      第71回日本体力医学会大会
    • Place of Presentation
      岩手県盛岡市
    • Year and Date
      2016-09-23
    • Related Report
      2016 Research-status Report
  • [Remarks] 滋賀県立大学 運動栄養学研究室

    • URL

      https://naoyanakai.wixsite.com/ex-nutrition

    • Related Report
      2018 Annual Research Report
  • [Remarks] 滋賀県立大学 人間文化学部 生活栄養学科 運動栄養学研究室

    • URL

      http://www.shc.usp.ac.jp/nakai/index.html

    • Related Report
      2017 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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