• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Comprehensive evaluation of the articular cartilage based on quantitative analysis of extracellular matrix using the new solubilization method.

Research Project

Project/Area Number 16K01821
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied health science
Research InstitutionShimane University

Principal Investigator

Kobayashi-Miura Mikiko  島根大学, 学術研究院医学・看護学系, 助教 (40447925)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords関節軟骨 / グリコサミノグリカン / コラーゲン / 発生 / 成熟 / グルコサミノグリカン / 老化 / 加齢
Outline of Final Research Achievements

We aimed to find an index for comprehensive estimation of the articular cartilage. We quantitated the glycosaminoglycan(GAG)and collagen(COL)as main extracellular matrix constituents of the articular cartilage at various growing stages during maturation in male rats. For COL quantitation, we measured hydroxyproline(Hyp), an unique modified amino acid in COL protein.
The amount of GAG was decreasing dramatically during the early maturation and then mildly at the latter half of maturation. On the other hand, the amount of Hyp was increasing constantly. The GAG/Hyp ratio is also dramatically changed during maturation and the ratio showed a unique value for each maturation stage clearly. Therefore, it is possible that the GAG/Hyp ratio may be a useful index for the estimation of the articular cartilage during maturation.

Academic Significance and Societal Importance of the Research Achievements

関節軟骨の摩耗が主な原因となる変形性膝関節症は日本人の5人に1人が罹患しているといわれ、特に高齢者に多い疾患である。しかし、関節軟骨の組織を形成する成分についての一生を通じての経時的な変化などについては未知の部分も多い。成熟に伴って組織の再性能が失われていくことから、成長過程の関節軟骨の性質を知ることは変形性膝関節症の治療法の開発などに繋がる可能性がある。本研究では、関節軟骨の細胞外基質組成分量とその組成比を成長段階ごとに解析し、関節軟骨組織の構成組成比GAG/Hypが未成熟な関節軟骨の成熟度を示す指標としての有用性を示唆した。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (19 results)

All 2020 2019 2018 2017 2016

All Journal Article (8 results) (of which Peer Reviewed: 7 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Quantitative analysis of the effects of nicotinamide phosphoribosyltransferase induction on the rates of NAD+ synthesis and breakdown in mammalian cells using stable isotope-labeling combined with mass spectrometry.2019

    • Author(s)
      Hara N, Osago H, Hiyoshi M, Kobayashi-Miura M, Tsuchiya M
    • Journal Title

      PLoS One

      Volume: 14 Issue: 3 Pages: e0214000-e0214000

    • DOI

      10.1371/journal.pone.0214000

    • NAID

      120006882593

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete solubilization of cartilage using the heat-stable protease thermolysin for comprehensive GAG analysis2018

    • Author(s)
      Osago H, Kobayashi-Miura M, Hamasaki Y, Hara N, Hiyoshi M, Tsuchiya M
    • Journal Title

      Analytical Biochemistry

      Volume: 548 Pages: 115-118

    • DOI

      10.1016/j.ab.2018.02.028

    • NAID

      120006882583

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Association of plasma free amino acids with hyperuricemia in relation to diabetes mellitus, dyslipidemia, hypertension and metabolic syndrome2017

    • Author(s)
      Mahbub MH, Yamaguchi N, Takahashi H, Hase R, Ishimaru Y, Sunagawa H, Amano H, Kobayashi-Miura M, Kanda H, Fujita Y, Yamamoto H, Yamamoto M, Kikuchi S, Ikeda A, Kageyama N, Nakamura M, Tanabe T
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s41598-017-17710-6

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Plasma free amino acid profiles evaluate risk of metabolic syndrome, diabetes, dyslipidemia, and hypertension in a large Asian population.2017

    • Author(s)
      Yamaguchi N, Mahbub MH, Takahashi H, Hase R, Ishimaru Y, Sunagawa H, Amano H, Kobayashi-Miura M, Kanda H, Fujita Y, Yamamoto H, Yamamoto M, Kikuchi S, Ikeda A, Takasu M, Kageyama N, Nakamura M, Tanabe T.
    • Journal Title

      Environ Health Prev Med.

      Volume: 22 Issue: 1 Pages: 22-35

    • DOI

      10.1186/s12199-017-0642-7

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Quantitative analyses of rat articular cartilage matrix during the maturation2017

    • Author(s)
      Kobayashi-Miura M, Osago H, Hiyoshi M, Hamasaki Y, Hara N, Tsuchiya M
    • Journal Title

      Mechanisms of Development

      Volume: 145 Pages: S131-S131

    • DOI

      10.1016/j.mod.2017.04.361

    • Related Report
      2017 Research-status Report
  • [Journal Article] Alteration in plasma free amino acid levels and its association with gout2017

    • Author(s)
      MH Mahbub, Natsu Yamaguchi, Hidekazu Takahashi, Ryosuke Hase, Hiroki Amano, Mikiko Kobayashi-Miura, Hideyuki Kanda, Yasuyuki Fujita, Hiroshi Yamamoto, Mai Yamamoto, Shinya Kikuchi, Atsuko Ikeda, Naoko Kageyama, Mina Nakamura, Yasutaka Ishimaru, Hiroshi Sunagawa, Tsuyoshi Tanabe
    • Journal Title

      Environmental Health and Preventive Medicine

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Plasma free amino acid profiles evaluate risk of metabolic syndrome, diabetes, dyslipidemia, and hypertension in a large Asian population2017

    • Author(s)
      Natsu Yamaguchi, M. H. Mahbub, Hidekazu Takahashi, Ryosuke Hase, Yasutaka Ishimaru, Hiroshi Sunagawa, Hiroki Amano, Mikiko Kobayashi- Miura, Hideyuki Kanda, Yasuyuki Fujita, Hiroshi Yamamoto, Mai Yamamoto, Shinya Kikuchi, Atsuko Ikeda, Mariko Takasu, Naoko Kageyama, Mina Nakamura, Tsuyoshi Tanabe
    • Journal Title

      Environmental Health and Preventive Medicine

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human thioredoxin-1 attenuates the rate of lipopolysaccharide-induced preterm delivery in mice in association with its anti-inflammatory effect.2016

    • Author(s)
      Namba F, Kobayashi-Miura M, Goda T, Nakura Y, Nishiumi F, Son A, Kubota A, Yodoi J, Yanagihara I.
    • Journal Title

      Pediatr Res.

      Volume: 80 Issue: 3 Pages: 433-439

    • DOI

      10.1038/pr.2016.100

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ラット硝子軟骨の各関節部位によるコラーゲン(COL)とグリコサミノグリカン(GAG)量の比較と加齢に伴う変化2020

    • Author(s)
      三浦美樹子, 長子晴美, 日吉峰麗, 原伸正, 土屋美加子
    • Organizer
      第52回日本結合組織学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The comparison of matrix component between male and female articular cartilage in rat femur of the knee joint2019

    • Author(s)
      Mikiko Kobayashi-Miura, Harumi Osago, Ikuko Takano, Yu Okada, Mineyoshi Hiyoshi, Nobumasa Hara, Mikako Tsuchiya
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 哺乳動物初代培養細胞におけるNAD+代謝の解析2019

    • Author(s)
      原伸正, 長子晴美, 日吉峰麗, 三浦美樹子, 土屋美加子
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ラット組織におけるコラーゲン、エラスチンのクロスリンク組成の比較2019

    • Author(s)
      長子晴美, 三浦美樹子, 河野通快, 原伸正, 日吉峰麗, 土屋美加子
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 新生仔から成獣におけるラット膝関節の大腿骨および脛骨関節軟骨のマトリックス構成成分の比較2018

    • Author(s)
      三浦 美樹子、長子 晴美、高野 育子, 日吉 峰麗, 原 伸正, 土屋 美加子
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 関節軟骨におけるグリコサミノグリカン組成2018

    • Author(s)
      長子 晴美、三浦 美樹子、原 伸正, 日吉 峰麗, 土屋 美加子
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] NAD+代謝におけるSIRT1の関与について2018

    • Author(s)
      原 伸正, 長子 晴美, 日吉 峰麗, 三浦 美樹子, 土屋 美加子
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Quantitative analyses of rat articular cartilage matrix during the maturation2017

    • Author(s)
      Kobayashi-Miura M, Osago H, Hiyoshi M, Hamasaki Y, Hara N, Tsuchiya M
    • Organizer
      the International Society of Developmental Biologists 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Qnatitative analyses of GAG and collagen in rat articular cartilage during the maturaion2017

    • Author(s)
      Kobayashi-Miura M, Osago H, Hiyoshi M, Hara N, Tsuchiya M
    • Organizer
      Consortium of Biological Sciences 2017
    • Related Report
      2017 Research-status Report
  • [Presentation] The characterization of matrix contents in the articular cartilage of the knee at the perinatal rats2016

    • Author(s)
      Mikiko Kobyaashi-Miura, Harumi Osago, Mineyoshi Hiyoshi, Nobumasa Hara, Mikako Tsuchiya
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台市 仙台国際センター 東北大学川内北キャンパス
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] 定量的オミクス解析のための結合組織の完全可溶化の試み2016

    • Author(s)
      長子 晴美、三浦 美樹子、濱崎 由文、原 伸正、日吉 峰麗、土屋 美加子
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台市 仙台国際センター 東北大学川内北キャンパス
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi