The role of lipid metabolism in the exercise-induced autophagy

• Project/Area Number: 16K01824
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Saga University
• Principal Investigator: Nishida Yuichiro 佐賀大学, 医学部, 講師 (50530185)
• Co-Investigator(Kenkyū-buntansha): 見市 文香 (三田村文香) 佐賀大学, 医学部, 講師 (70576818)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 運動誘発性オートファジー / 有酸素運動 / 脂質代謝 / 生理学

Outline of Final Research Achievements

The current study investigated the effects of low-intensity exercise (10 m/min, 90 min) and high-intensity exercise (15 m/min, 60 min) on the autophagy activation in the liver and skeletal muscle (gastrocnemius muscle) and explored the mechanisms underlying the exercise-induced autophagy activation in male GFP-LC3 mice.
Autophagy activation was assessed by the intensity of GFP in the liver and skeletal muscle. The current results suggest that the high-intensity exercise is more effective in increasing the autophagy in the liver in comparison to low-intensity exercise, whereas the low-intensity exercise is more effective for increasing the autophagy in the skeletal muscle in comparison to high-intensity exercise.

Academic Significance and Societal Importance of the Research Achievements

先行研究により、一過性の運動によるインスリン作用の改善に骨格筋におけるオートファジーの活性化が必須であるということが報告されたが、骨格筋のオートファジーを最も効果的に活性化する運動条件については全くわかっていなかった。本研究の結果は、骨格筋のオートファジー活性化には低強度運動の方が効果が高い可能性と、肝臓のオートファジー活性化には高強度運動の方が効果が高い可能性を示唆するものである。