| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The Lasso peptide is a kind of cyclic peptide, and it is known to exert various pharmacological activities. In this study, we screened for new lasso peptides using genetic information (genome mining) and succeeded in obtaining many new lasso peptides mainly from actinomycetes. We also worked on the development of a heterologous production method using a soil bacterium Sphingomonas as a host, and succeeded in producing the new lasso peptide named brevunsin. The obtained rasso peptide was subjected to structure determination using NMR and ESI-MS, and the three-dimensional three-dimensional structure was successfully determined based on the NOE experiments.
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