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Molecular evolution of precursor protein of peptide hormone and its application for molecular modeling

Research Project

Project/Area Number 16K01925
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomolecular chemistry
Research InstitutionKindai University

Principal Investigator

Hidaka Yuji  近畿大学, 理工学部, 教授 (70212165)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords前駆体 / フォールディング / ペプチドホルモン / ジスルフィド / 分子進化 / シャペロン / ウログアニリン
Outline of Final Research Achievements

To investigate how peptide hormones develop their biological activity on molecular evolution, the folding mechanism of the precursor proteins, such as prouroguanylin and pro-ANP were investigated.
Structural analyses of the proteins by means of NMR and X-ray crystallography including their folding intermediates suggested that the secondary structural shift at the hinge region of the processing sites maintains the correct conformation of the final folding products during the molecular evolution. In addition,the propeptide region was fused to de novo designed peptides to regulate the bioactive conformation, resulting in the stabilization of the correct conformation of the de novo peptide. Thus, we successfully obtained the bioactive de novo peptides.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、分子進化において、これまで殆ど情報が得られていない蛋白質の立体構造形成レベルでの情報を与えるものである。また、本研究の独創性として、その分子進化のターゲットをペプチドホルモンの前駆体としたことから、そのプロセッシング機構を伴う、真の意味での分子進化を解明することができた。本観点から、様々なペプチドホルモンについて検討を行った結果、それら分子進化と生理活性構造の品質管理機構が、プロセッシング部位そのものにあることを確定し、今後の分子モデリングにおける分子内シャペロンの重要性を提示するに至った。
本概念に基づき、新たな人工設計生理活性ペプチドを獲得し、今後の医薬の開発に貢献できた。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (76 results)

All 2020 2019 2018 2017 2016

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (59 results) (of which Int'l Joint Research: 18 results)

  • [Journal Article] Cloning and Functional Analysis of Digestive Enzyme Derived from Nephila Clavata2019

    • Author(s)
      Tsubasa Tagawa , Teruki Hagiwara , Shigeru Shimamoto , Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2018 Pages: 101-101

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Control and Functional Analysis of the Precursor Protein of the Atrial Natriuretic Peptide2019

    • Author(s)
      Hayato Ueda , Shigeru Shimamoto , Yuji HidakaHayato Ueda , Shigeru Shimamoto , Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2018 Pages: 94-94

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Analyses of an N-terminal Extracellular Domain of the Amyloid Precursor Protein2019

    • Author(s)
      Mizuho Imamura , Shingo Kanemura , Masaki Okumura , Hiroshi Yamaguchi , Shigeru Shimamoto , Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2018 Pages: 23-23

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Coupling effects of thiol and urea-type groups for promotion of oxidative protein folding2019

    • Author(s)
      Shunsuke Okada, Motonori Matsusaki, Kenta AraiK , Yuji Hidaka, Kenji Inaba, Masaki Okumura, Takahiro Muraoka
    • Journal Title

      Chemical Communications

      Volume: 55(6) Issue: 6 Pages: 759-762

    • DOI

      10.1039/c8cc08657e

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cloning and Functional Analaysis of Digestive Enzyme Derived from Nephila Clavata2019

    • Author(s)
      Tsubasa Tagawa, Teruki Hagiwara, Shigeru Shimamoto and Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2018

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural Analyses of An N-terminal Extracellular Domain of The Amyloid Precursor Protein2019

    • Author(s)
      Mizuho Imamura, Shingo Kanemura, Masaki Okumura, Hiroshi Yamaguchi, Shigeru Shimamoto, Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2018

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural Control And Functional Analysis Of The Precursor Protein Of Atraial Natriuretic Peptide2019

    • Author(s)
      Hayato Ueda, Shigeru Shimamoto, Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2018

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural Analyses of the Linker Region of the Amyloid Precursor Protein2018

    • Author(s)
      Mizuho Imamura, Shingo Kanemura, Masaki Okumura,Shigeru Shimamoto, and Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2017 Pages: 206-207

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of Disulfide-Coupled Folding of a De Novo Designed Protein2018

    • Author(s)
      Saya Nishihara, Kosuke Toyama, Kenta Mori, Shigeru Shimamoto, Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2017 Pages: 208-209

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular Evolution of L-PGDS: Substrate Recognition Mechanism of Medaka L-PGDS2018

    • Author(s)
      Kimi Torii, Takahiro Maruno, Yuji Kobayashi, Yuji Hidaka, and Shigeru Shimamoto
    • Journal Title

      Peptide Science

      Volume: 2017 Pages: 210-211

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Disulfide-Coupled Folding of Pro-Uroguanylin on Molecular Evolution2018

    • Author(s)
      Kenta Mori, Kosuke Toyama, Saya Nishihara, Shigeru Shimamoto, Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2017 Pages: 214-215

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gene expression of the concentration-sensitive sodium channel is suppressed in lipopolysaccharide-induced acute lung injury in mice2017

    • Author(s)
      Hagiwara Teruki、Yoshida Shigeru、Hidaka Yuji
    • Journal Title

      Experimental Lung Research

      Volume: 43 Issue: 3 Pages: 150-157

    • DOI

      10.1080/01902148.2017.1321064

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Disulfide-Coupled Folding of Pro-Uroguanylin on Molecular Evolution2017

    • Author(s)
      Kenta Mori, Kosuke Toyama, Shigeru Shimamoto, Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2016 Pages: 103-104

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Activation mechanism of Cocoonase2017

    • Author(s)
      Nagisa Tajima, Shigeru Shimamoto, Mitsuhiro Miyazawa, Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2016 Pages: 105-106

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Preparation of an Orexin Precorsor Protein Using an E. coli Expression System by Acid Treatment2017

    • Author(s)
      Natsumi Mitsuoka, Shigeru Shimamoto, Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2016 Pages: 221-222

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of the Disulfide-Coupled Folding of De Novo Designed Peptides by Alpha-Helix Formation2017

    • Author(s)
      Saya Nishihara, Kosuke Toyama, Shigeru Shimamoto, Yuji Hidaka
    • Journal Title

      Peptide Science

      Volume: 2016 Pages: 223-224

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quantification of DNA damages by real-time PCR reaction and its application to radiation monitoring system2016

    • Author(s)
      Kikuo Shimizu, Ryuto Nakashima, Youichirou Matuo, Yuji Hidaka, Norihito Saito, Takayoshi Yamamoto
    • Journal Title

      日本放射線安全学会誌

      Volume: 15 Pages: 52-58

    • NAID

      130006895871

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] INTRA-MOLECULAR CHAPERONE MEDIATED FOLDING OF A PEPTIDE HORMONE IN MOLECULAR EVOLUTION2020

    • Author(s)
      Toi Osumi, Aman L. Maharjan, Mayu Fukutsuji, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      64th Annual Meeting of the Biophysical Society
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] MECHANISM OF THE DISULFIDE-COUPLED FOLDING OF A DE NOVO DESIGNED PROUROGUANYLIN PROTEIN2020

    • Author(s)
      Mayu Fukutsuji, Aman L. Maharjan, Toi Osumi, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      64th Annual Meeting of the Biophysical Society
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] EXPLORING SERUM PROTEINS TO STABILIZE THE CONFORMATION OF THE PRECURSOR PROTEIN OF ANP2020

    • Author(s)
      Yuji Hidaka, Hayato Ueda, Shigeru Shimamoto
    • Organizer
      64th Annual Meeting of the Biophysical Society
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ENZYME ACTIVATION MECHANISM OF COCOONASE2020

    • Author(s)
      Mai Takegawa, Tsubasa Tagawa, Ayumi Ogata, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      64th Annual Meeting of the Biophysical Society
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PDIファミリーが触媒する前駆体タンパク質の酸化的フォールディング機構の解明2019

    • Author(s)
      増田光紀, 木下 岬, 山口 宏, 日高雄二, 稲葉謙次, 奥村正樹
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 新規レドックス分子のデザイン法確立と酸化的フォールディングへの応用2019

    • Author(s)
      松崎元紀, 岡田隼輔, 荒井堅太, 日高雄二, 稲葉謙次, 村岡貴博, 奥村正樹
    • Organizer
      第92回 日本生化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] メダカ由来プロスタグランジン結合蛋白質の機能解明2019

    • Author(s)
      岩壁一樹, 鳥居希美, 島本 茂, 日高雄二
    • Organizer
      第51回若手ペプチド夏の勉強会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ペプチドホルモン前駆体タンパク質の生理活性構造形成機構2019

    • Author(s)
      大角, 泰唯, 島本 茂, 日高雄二
    • Organizer
      第51回若手ペプチド夏の勉強会
    • Related Report
      2019 Annual Research Report
  • [Presentation] カイココクナーゼの酵素活性化機構の解明2019

    • Author(s)
      竹川 舞, 島本 茂, 日高雄二
    • Organizer
      第51回若手ペプチド夏の勉強会
    • Related Report
      2019 Annual Research Report
  • [Presentation] de novo デザイン蛋白質の立体構造形成機構の解明2019

    • Author(s)
      福辻真由, 島本 茂, 日高雄二
    • Organizer
      第51回若手ペプチド夏の勉強会
    • Related Report
      2019 Annual Research Report
  • [Presentation] タンパク質へのSeCys残基の選択的導入の検討2019

    • Author(s)
      藤田克也, 島本 茂, 日高雄二
    • Organizer
      第51回若手ペプチド夏の勉強会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 心房性ナトリウム利尿ペプチド前駆体タンパク質の構造制御および機能解析2019

    • Author(s)
      上田 隼, 島本 茂, 日高雄二
    • Organizer
      第51回若手ペプチド夏の勉強会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 分子進化におけるプロウログアニリンの立体構造形成機構の解明2019

    • Author(s)
      マハラジャン アマン ラル, 島本 茂, 日高雄二
    • Organizer
      第51回若手ペプチド夏の勉強会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 心房性ナトリウム利尿ペプチド前駆体の構造制御及び機能解析2019

    • Author(s)
      上田 隼, 島本 茂, 日高 雄二
    • Organizer
      第19回日本蛋白質科学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ジョロウグモ由来消化酵素のクローニングおよび機能解析2019

    • Author(s)
      田川 翼, 萩原 央記, 宮澤 光博, 島本 茂, 日高 雄二
    • Organizer
      第19回日本蛋白質科学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] PDI ファミリーによる前駆体タンパク質フォールディング中間体の分子認識機構の解明2019

    • Author(s)
      増田 光紀, 金村 進吾, 木下 岬, 山口 宏, 日高 雄二, 稲葉 謙次, 奥村 正樹
    • Organizer
      第19回日本蛋白質科学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 新規レドックス分子による酸化的フォールディングの促進と応用2019

    • Author(s)
      松崎 元紀, 岡田 隼輔, 荒井 堅太, 日高 雄二, 稲葉 謙次, 村岡 貴博, 奥村 正樹
    • Organizer
      第19回日本蛋白質科学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 造血器型プロスタグランジンD 合成酵素と補酵素・基質の分子認識機構と反応機構の解明2019

    • Author(s)
      東野 貴大, 島本 茂, 日高 雄二
    • Organizer
      第19回日本蛋白質科学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] メダカ由来プロスタグランジンD 合成酵素類縁体の機能解明2019

    • Author(s)
      岩壁 一樹, 鳥居 希美, 島本 茂, 加川 尚, 日高 雄二
    • Organizer
      第19回日本蛋白質科学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 等温滴定型熱測定による睡眠物質合成酵素と基質の相互作用解析2019

    • Author(s)
      島本 茂, 中川 悠介, 日高 雄二, 丸野 孝浩, 小林 祐次, 河原 一樹, 吉田 卓也, 大久保 忠恭, 有竹 浩介, 裏出 良博
    • Organizer
      第19回日本蛋白質科学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] QUALITY CONTROL OF DISULFIDE-COUPLED FOLDING OF A PEPTIDE HORMONE, UROGUANYLIN, ON MOLECULAR EVOLUTION2019

    • Author(s)
      Aman Lall Maharjan, Toi Osumi, Mayu Fukutsuji, Shigeru Shimamoto, and Yuji Hidaka
    • Organizer
      第56回ペプチド討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] MECHANISM OF THE DISULFIDE-COUPLED FOLDING OF A DE NOVO DESIGNED PROUROGUANYLIN PROTEIN2019

    • Author(s)
      Mayu Fukutsuji, A. L. Maharjan, Toi Osumi, Saya Nishihara, Shigeru Shimamoto, and Yuji Hidaka
    • Organizer
      第56回ペプチド討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] CLONING AND FUNCTIONAL ANALYSES OF DIGESTIVE ENZYMES DERIVED FROM NEPHILA CLAVATA2019

    • Author(s)
      Tsubasa Tagawa, Teruki Hagiwara, Mitsuhiro Miyazawa, Shigeru Shimamoto, and Yuji Hidaka
    • Organizer
      第56回ペプチド討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ENZYME ACTIVATION MECHANISM OF COCOONASE2019

    • Author(s)
      Mai Takegawa, TsubasaTagawa, Ayumi Ogata, Mitsuhiro Miyazawa, Shigeru Shimamoto, and Yuji Hidaka
    • Organizer
      第56回ペプチド討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 前駆体タンパク質のプロ領域が制御するフォールディング機構の解明2018

    • Author(s)
      小林 優真、奥村 正樹、島本 茂、稲葉 謙次、山口 宏、日高 雄二
    • Organizer
      第18回日本蛋白質科学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Cloning and Functional Analaysis of Digestive Enzyme Derived from Nephila Clavata2018

    • Author(s)
      Tsubasa Tagawa, Teruki Hagiwara, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      The 10th International Peptide Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Structural Analyses of An N-terminal Extracellular Domain of The Amyloid Precursor Protein2018

    • Author(s)
      Mizuho Imamura, Shingo Kanemura, Masaki Okumura, Hiroshi Yamaguchi, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      The 10th International Peptide Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Structural Control And Functional Analysis of The Precursor Protein of Atraial Natriuretic Peptide2018

    • Author(s)
      Hayato Ueda, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      The 10th International Peptide Symposium
    • Related Report
      2018 Research-status Report
  • [Presentation] βアミロイド前駆体蛋白質N末端ドメインの構造解析2018

    • Author(s)
      今村瑞穂、島本 茂、日髙雄二
    • Organizer
      第35回 関西地区ペプチドセミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] L-PGDSの分子進化:メダカL-PGDSの基質認識機構2018

    • Author(s)
      鳥居希美、島本 茂、日髙雄二
    • Organizer
      第35回 関西地区ペプチドセミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] 心房性ナトリウム利尿ペプチド前駆体の構造制御および機能解析2018

    • Author(s)
      上田 隼、島本 茂、日髙雄二
    • Organizer
      第35回 関西地区ペプチドセミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] ジョロウグモ由来酵素のクローニングおよび機能解析2018

    • Author(s)
      田川 翼、島本 茂、日髙雄二
    • Organizer
      第35回 関西地区ペプチドセミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] 蛋白質へのSeCys残基の選択的導入の検2018

    • Author(s)
      藤田克也、島本 茂、日髙雄二
    • Organizer
      第35回 関西地区ペプチドセミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] 分子進化におけるプロウログアニリンの立体構造形成機構の解明2018

    • Author(s)
      MAHARJAN AMAN LALL、島本 茂、日髙雄二
    • Organizer
      第35回 関西地区ペプチドセミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] MOLECULAR EVOLUTION OF L-PGDS: SUBSTRATE RECOGNITION MECHANISM2018

    • Author(s)
      Kimi Torii, Yuji Hidaka, Shigeru Shimamoto
    • Organizer
      62nd Annual meeting of Biophysical Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] DISULFIDE-COUPLED FOLDING OF PROUROGUANYLIN ON MOLECULAR EVOLUTION2018

    • Author(s)
      Kenta Mori, Saya Nishihara, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      62nd Annual meeting of Biophysical Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] FOLDING ANALYSES OF A DE NOVO DESIGNED PROUROGUANYLIN2018

    • Author(s)
      Yuji Hidaka, Saya Nishihara, Kenta Mori, Shigeru Shimamoto
    • Organizer
      62nd Annual meeting of Biophysical Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] STRUCTURAL ANALYSES OF A LINKER REGION OF THE AMYLOID PRECURSOR2018

    • Author(s)
      Mizuho Imamura, Shingo Kanemura, Masaki Okumura,Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      62nd Annual meeting of Biophysical Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ACTIVATION MECHANISM OF COCOONASE2018

    • Author(s)
      Nagisa Tajima, Mitsuhiro Miyazawa, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      62nd Annual meeting of Biophysical Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] MOLECULAR RECOGNITION MECHANISM OF HEMATOPOIETIC PROSTAGLANDIN AND ITS SUBSTRATE2018

    • Author(s)
      Shigeru Shimamoto, Keisuke Asada, Yuji Hidaka
    • Organizer
      62nd Annual meeting of Biophysical Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Regulation of protein folding using organic solvents and ionic liquids2017

    • Author(s)
      Yuji Hidaka, Wataru Ito, Ryosuke, Nishimura, Shigeru Shimamoto
    • Organizer
      Biophysical Society 61st Annual Meeting
    • Place of Presentation
      ニューオーリンズ(米国)
    • Year and Date
      2017-02-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Structural analysis of the precursor protein of the atrial natriuretic peptide2017

    • Author(s)
      Sumika Futori, Satomi Higashigawa, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      Biophysical Society 61st Annual Meeting
    • Place of Presentation
      ニューオーリンズ(米国)
    • Year and Date
      2017-02-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular recognition mechanism of Hematopoietic Prostaglandin D Synthase with its cofactor and its substrate2017

    • Author(s)
      eisuke Asada, Shigeru Shimamoto, Tomohiro Oonoki, Takahiro Maruno, Yuji Kobayashi, Kosuke Aritake, Yoshihiro Urade, Yuji Hidaka
    • Organizer
      Biophysical Society 61st Annual Meeting
    • Place of Presentation
      ニューオーリンズ(米国)
    • Year and Date
      2017-02-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Preparation of an Orexin Precorsor Protein by Chemical Digestion2017

    • Author(s)
      Natsumi Mitsuoka, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      Biophysical Society 61st Annual Meeting
    • Place of Presentation
      ニューオーリンズ(米国)
    • Year and Date
      2017-02-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Regulation of Folding of De Novo Designed Peptides by Alpha-Helix Formation2017

    • Author(s)
      Saya Nishihara, Kosuke Toyama, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      Biophysical Society 61st Annual Meeting
    • Place of Presentation
      ニューオーリンズ(米国)
    • Year and Date
      2017-02-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Lipocalin-Type Prostaglandin D Synthase Possesses Two Binding Sites for its product.2017

    • Author(s)
      Shigeru Shimamoto, Yusuke Nakagawa, Yuta Nakahata,Takahiro Maruno, Yuji Kobayashi, Kosuke Aritake, Yoshihiro Urade, Yuji Hidaka
    • Organizer
      Biophysical Society 61st Annual Meeting
    • Place of Presentation
      ニューオーリンズ(米国)
    • Year and Date
      2017-02-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] STRUCTURAL ANALYSES OF A LINKER REGION OF AMYLOID PRECURSOR PROTEIN2017

    • Author(s)
      Mizuho Imamura, Shingo Kanemura, Masaki Okumura,Shigeru Shimamoto, and Yuji Hidaka
    • Organizer
      第54回ペプチド討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] Disulfide-Coupled Folding of Pro-Uroguanylin on Molecular Evolution2017

    • Author(s)
      Kenta Mori, Kosuke Toyama, Saya Nishihara, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      第54回ペプチド討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] Molecular Evolution of L-PGDS: Substrate Recognition Mechanism of Medaka L-PGDS2017

    • Author(s)
      Kimi Torii, Takahiro Maruno, Yuji Kobayashi, Yuji Hidaka, and Shigeru Shimamoto
    • Organizer
      第54回ペプチド討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] Regulation of Disulfide-Coupled Folding of a De Novo Designed Protein2017

    • Author(s)
      Saya Nishihara, Kosuke Toyama, Kenta Mori, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      第54回ペプチド討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] 分子進化におけるプロウログアニリンの立体構造形成機構の解明2017

    • Author(s)
      森 健太、日高 雄二
    • Organizer
      第34回関西地区ペプチドセミナー
    • Related Report
      2017 Research-status Report
  • [Presentation] プロペプチドによるカイココクナーゼの立体構造形成機構の解明2017

    • Author(s)
      田嶋 渚、日高 雄二
    • Organizer
      第34回関西地区ペプチドセミナー
    • Related Report
      2017 Research-status Report
  • [Presentation] de novo設計蛋白質の立体構造形成機構の解明2017

    • Author(s)
      西原 紗彩、日高 雄二
    • Organizer
      第34回関西地区ペプチドセミナー
    • Related Report
      2017 Research-status Report
  • [Presentation] L-PGDSの分子進化:メダカL-PGDSの基質認識機構2017

    • Author(s)
      鳥居 希美、日高 雄二、島本 茂
    • Organizer
      第34回関西地区ペプチドセミナー
    • Related Report
      2017 Research-status Report
  • [Presentation] アミロイドβ前駆体蛋白質のリンカー領域の立体構造解析2017

    • Author(s)
      今村 瑞穂、日高 雄二
    • Organizer
      第34回関西地区ペプチドセミナー
    • Related Report
      2017 Research-status Report
  • [Presentation] Disulfide-Coupled Folding of Pro-Uroguanylin on Molecular Evolution2016

    • Author(s)
      Kenta Mori, Kosuke Toyama, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      第52回ペプチド討論会
    • Place of Presentation
      京都テルサ(京都)
    • Year and Date
      2016-10-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Activation mechanism of Cocoonase2016

    • Author(s)
      Nagisa Tajima, Shigeru Shimamoto, Mitsuhiro Miyazawa, Yuji Hidaka
    • Organizer
      第52回ペプチド討論会
    • Place of Presentation
      京都テルサ(京都)
    • Year and Date
      2016-10-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Preparation of an Orexin Precorsor Protein Using an E. coli Expression System by Acid Treatment2016

    • Author(s)
      Natsumi Mitsuoka, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      第52回ペプチド討論会
    • Place of Presentation
      京都テルサ(京都)
    • Year and Date
      2016-10-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Regulation of the Disulfide-Coupled Folding of De Novo Designed Peptides by Alpha-Helix Formation2016

    • Author(s)
      Saya Nishihara, Kosuke Toyama, Shigeru Shimamoto, Yuji Hidaka
    • Organizer
      第52回ペプチド討論会
    • Place of Presentation
      京都テルサ(京都)
    • Year and Date
      2016-10-26
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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