| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
To investigate how peptide hormones develop their biological activity on molecular evolution, the folding mechanism of the precursor proteins, such as prouroguanylin and pro-ANP were investigated.
Structural analyses of the proteins by means of NMR and X-ray crystallography including their folding intermediates suggested that the secondary structural shift at the hinge region of the processing sites maintains the correct conformation of the final folding products during the molecular evolution. In addition,the propeptide region was fused to de novo designed peptides to regulate the bioactive conformation, resulting in the stabilization of the correct conformation of the de novo peptide. Thus, we successfully obtained the bioactive de novo peptides.
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