| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
This study aimed to develop a methodology to regulate the target signal transduction in the cell by using site-directed RNA editing technology that utilizes endogenous RNA editing mechanism. In the previous our research, we have established basic methodology for site-directed RNA editing with the guide RNA (AD-gRNA) that could induce the RNA editing activity of adenosine deaminase acting on RNA into the target-site. In order to improve this site-directed RNA editing to apply for the regulation of intracellular signaling pathway, we newly designed the short-type AD-gRNA (shAD-gRNA) with the equal activity of the original gRNA. shAD-gRNAs succeeded in site-directed RNA editing in the intracellular environment and could be applied to the regulation of target gene expression in ADAR-expressing cells.
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