| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
In this study, we aim to develop fluorescence imaging of cellular histone deacetylase (HDAC) activity that involves in cancer and neurodegenerative diseases. The fluorescent probe increases DNA binding affinity and fluorescence intensity upon deacetylation of substrate. Modification of the probe structure by introduction of the substructure of HDAC inhibitor expanded the HDAC selectivity. We also improved turn-on ratio of the fluorescence signals by introducing multiple acetyllysine substrates. As a result, nuclear HDAC activity from HeLa cells was fluorescently detected using the modified fluorescent probe.
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