Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Outline of Final Research Achievements |
The aim of this study is to be clarified the mechanisms that the thiol (containing -SH group) and persulfide (containing -SSH group that is one of the most powerful anti-oxidant thiol compounds) species could reduce intracellular oxidative stresses in the ischemic diseases. First, we performed measurements of the thiol and persulfide in the ischemic mice heart, suggesting that many thiol-containing metabolites play important roles to prevent from heart failure. Besides quantifications of thiol-compounds, we tried the MALDI (Matrix-Assisted Laser/Desorption Ionization) MS imaging for the visualization of the thiol and persulfide on the tissue sections. However, MALDI-MS could oxidize the thiol group easily, making us underestimate the contents of thiol compound on the tissue. Therefore, we apply another method, SERS (Surface-enhanced Raman spectroscopy) imaging technology. SERS enabled us to visualize the tissue distribution of the thiol and persulfide without labelling or staining.
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