| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
This project is intended to find a clue for the understanding how sensory signals are processed in the brain, by using Photometric patch electrode (PME) recording system which enables recordings of neural electrical and Ca fluorescence signals simultaneously in the deep brain region. Ca signals were measured by using the indicator either GCaMP6 or OGB-1-AM. GCaMP6 was expressed in adult mice hippocampus and measured the neural spontaneous activity or sensory evoked activity in awake mice. Processing of auditory information was studied in field L (avian auditory cortex) of the chick after acute injection of OGB-1-AM. Ascending auditory projection to field L was separately visualized by mCherry expressed by Avian Adeno Associated virus. Furthermore, application of PME was extended to olfactory receptor epithelium for promoting the technology among other researchers in the field of neuroscience.
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