Tow-dimensional membrane scaffolds for oriented immobilization of various sensing molecules

• Project/Area Number: 16K04888
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nanomaterials chemistry
• Research Institution: Tokyo University of Agriculture (2018-2019); Osaka University (2016-2017)
• Principal Investigator: IIJIMA Masumi 東京農業大学, 応用生物科学部, 准教授 (40390728)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ナノバイオ / ナノ材料 / バイオテクノロジー / 生体分子 / 酵素反応

Outline of Final Research Achievements

Both clustering and oriented immobilization of enzymes on solid phase are critical for improving the enzyme-catalyzed chemical transformation of biosensors. Here, we have developed novel bio-nanocapusle-based scaffolds for clustering and oriented immobilization of enzymes. Furthermore, two-dimensional membrane scaffolds have also been developed for the oriented immobilization of biosensing molecules. These achievements could be applicable to develop the nanoblock scaffolds which could ordered-immobilize various biosensing molecules.

Academic Significance and Societal Importance of the Research Achievements

本研究成果によって確立された新規足場分子による酵素の精密整列提示方法、さらに、2次元膜上におけるセンシング分子の精密整列提示方法は、他に類を見ない技術であり、その独自性や優位性は非常に高いと考える。今後、本技術は、様々なデバイス（バイオセンサー、バイオリアクター、MEMS、μ-TAS等）の固相表面での超効率的な反応、触媒、エネルギー変換、物質生産などへの応用も期待できることから、その学術的意義や社会的意義は高いと考える。

Research Products

• [Journal Article] Two-dimensional membrane scaffold for the oriented immobilization of biosensing molecules (2020)
  • Author(s): Iijima Masumi、Nakayama Tsutomu、Kuroda Shun'ichi
  • Journal Title: Biosensors and Bioelectronics Volume: 150 Pages: 111860-111860
  • DOI: 10.1016/j.bios.2019.111860
  • Peer Reviewed
• [Journal Article] Oriented immobilization to nanoparticles enhanced the therapeutic efficacy of antibody drugs (2019)
  • Author(s): Iijima M, Araki K, Liu Q, Somiya M, Kuroda S
  • Journal Title: Acta Biomaterialia Volume: 86 Pages: 373-380
  • DOI: 10.1016/j.actbio.2019.01.011
  • Peer Reviewed
• [Journal Article] The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis (2019)
  • Author(s): Yamagishi Ayana、Susaki Moe、Takano Yuta、Mizusawa Mei、Mishima Mari、Iijima Masumi、Kuroda Shun'ichi、Okada Tomoko、Nakamura Chikashi
  • Journal Title: International Journal of Biological Sciences Volume: 15 Issue: 7 Pages: 1546-1556
  • DOI: 10.7150/ijbs.33423
  • Peer Reviewed / Open Access
• [Journal Article] Ultrahigh-sensitive wireless QCM with bio-nanocapsules (2019)
  • Author(s): Noi Kentaro、Iijima Masumi、Kuroda Shun’ichi、Ogi Hirotsugu
  • Journal Title: Sensors and Actuators B: Chemical Volume: 293 Pages: 59-62
  • DOI: 10.1016/j.snb.2019.04.150
  • NAID: 120007148402
  • Peer Reviewed
• [Journal Article] In vivo uterine local gene delivery system using TAT‐displaying bionanocapsules (2019)
  • Author(s): Koizumi Kaori、Nakamura Hitomi、Iijima Masumi、Matsuzaki Takashi、Somiya Masaharu、Kumasawa Keiichi、Kimura Tadashi、Kuroda Shun'ichi
  • Journal Title: The Journal of Gene Medicine Volume: 21 Issue: 12
  • DOI: 10.1002/jgm.3140
  • Peer Reviewed
• [Journal Article] A hepatitis B virus-derived human hepatic cell-specific heparin-binding peptide: identification and application to a drug delivery system (2019)
  • Author(s): Liu Qiushi、Somiya Masaharu、Iijima Masumi、Tatematsu Kenji、Kuroda Shun'ichi
  • Journal Title: Biomaterials Science Volume: 7 Issue: 1 Pages: 322-335
  • DOI: 10.1039/c8bm01134f
  • Peer Reviewed
• [Journal Article] Development of a Macrophage-Targeting and Phagocytosis-Inducing Bio-nanocapsule-based Nanocarrier for Drug Delivery (2018)
  • Author(s): Li Hao、Tatematsu Kenji、Somiya Masaharu、Iijima Masumi、ichi Kuroda Shun'
  • Journal Title: Acta Biomaterialia Volume: 490 Pages: 155-160
  • DOI: 10.1016/j.actbio.2018.04.023
  • Peer Reviewed
• [Journal Article] CD11c-specific bio-nanocapsule enhances vaccine immunogenicity by targeting immune cells (2018)
  • Author(s): Matsuo Hidenori、Somiya Masaharu、Iijima Masumi、Arakawa Takeshi、Kuroda Shun’ichi
  • Journal Title: Journal of Nanobiotechnology Volume: 16 Issue: 1 Pages: 59-59
  • DOI: 10.1186/s12951-018-0386-6
  • Peer Reviewed
• [Journal Article] Scaffolds for oriented and close-packed immobilization of immunoglobulins (2017)
  • Author(s): Iijima Masumi、Kuroda Shun’ichi
  • Journal Title: Biosensors and Bioelectronics Volume: 89 Pages: 810-821
  • DOI: 10.1016/j.bios.2016.10.009
  • Peer Reviewed
• [Journal Article] Synthesis and assembly of Hepatitis B virus envelope protein-derived particles in Escherichia coli (2017)
  • Author(s): Li Hao、Onbe Keisuke、Liu Qiushi、Iijima Masumi、Tatematsu Kenji、Seno Masaharu、Tada Hiroko、Kuroda Shun’ ichi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 490 Issue: 2 Pages: 155-160
  • DOI: 10.1016/j.bbrc.2017.06.015
  • Peer Reviewed / Open Access
• [Journal Article] A New Cell Separation Method Based on Antibody-Immobilized Nanoneedle Arrays for the Detection of Intracellular Markers (2017)
  • Author(s): Kawamura Ryuzo、Miyazaki Minami、Shimizu Keita、Matsumoto Yuta、Silberberg Yaron R.、Sathuluri Ramachandra Rao、Iijima Masumi、Kuroda Shun’ichi、Iwata Futoshi、Kobayashi Takeshi、Nakamura Chikashi
  • Journal Title: Nano Letters Volume: 17 Issue: 11 Pages: 7117-7124
  • DOI: 10.1021/acs.nanolett.7b03918
  • Peer Reviewed
• [Journal Article] Cytokine-dependent activation of the JAK-STAT pathway in Saccharomyces cerevisiae (2016)
  • Author(s): Yoshimoto, N., Ikeda, Y., Tatematsu, K., Iijima, M., Nakai, T., Okajima, T., Tanizawa, K., Kuroda, S.
  • Journal Title: Biotechnology and Bioengineering Volume: 印刷中 Issue: 8 Pages: 1796-1804
  • DOI: 10.1002/bit.25948
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Bio-nanocapsules displaying various immunoglobulins as an active targeting-based drug delivery system (2016)
  • Author(s): Kenji Tatematsu, Masumi Iijima, Nobuo Yoshimoto, Tadashi Nakai, Toshihide Okajima, Shun’ichi Kuroda
  • Journal Title: Acta Biomaterialia Volume: 35 Pages: 238-247
  • DOI: 10.1016/j.actbio.2016.02.010
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Bio-nanocapsule-based scaffold improves the sensitivity and ligand-binding capacity of mammalian receptors on the sensor chip (2016)
  • Author(s): Iijima, M., Yoshimoto, N., Niimi, T., Maturana, A.D., Kuroda, S.
  • Journal Title: Biotechnology journal Volume: 印刷中 Issue: 6 Pages: 805-813
  • DOI: 10.1002/biot.201500443
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 生体分子の整列固定化法 (2016)
  • Author(s): 飯嶋益巳、黒田俊一
  • Journal Title: 生物工学会誌 Volume: 94 Pages: 497-497
  • Peer Reviewed
• [Journal Article] Scaffold protein enigma homolog 1 overcomes the repression of myogenesis activation by inhibitor of DNA binding 2 (2016)
  • Author(s): Nakatani M. Ito J. Koyama R. Iijima M. Yoshimoto N. Niimi T. Kuroda S. Maturana AD
  • Journal Title: Biochem Biophys Res Commun. Volume: 0 Issue: 2 Pages: 0-0
  • DOI: 10.1016/j.bbrc.2016.04.119
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mutational analysis of hepatitis B virus pre-S1 (9-24) fusogenic peptide (2016)
  • Author(s): Qiushi Liu, Masaharu Somiya, Naohiko Shimada, Wakako Sakamoto, Nobuo Yoshimoto, Masumi Iijima, Kenji Tatematsu, Tadashi Nakai, Toshihide Okajima, Atsushi Maruyama, Shun'ichi Kuroda
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 474 Issue: 2 Pages: 406-412
  • DOI: 10.1016/j.bbrc.2016.04.125
  • Peer Reviewed / Open Access
• [Journal Article] RBM20 and RBM24 cooperatively promote the expression of short enh splice variants (2016)
  • Author(s): Jumpei Ito, Masumi Iijima, Nobuo Yoshimoto, Tomoaki Niimi, Shun'ichi Kuroda, Andres D. Maturana
  • Journal Title: FEBS Letter Volume: 590 Issue: 14 Pages: 2262-2274
  • DOI: 10.1002/1873-3468.12251
  • Peer Reviewed / Open Access
• [Journal Article] Core-fucosylation plays a pivotal role in hepatitis B pseudo virus infection: a possible implication for HBV glycotherapy. (2016)
  • Author(s): Takamatsu S, Shimomura M, Kamada Y, Maeda H, Sobajima T, Hikita H, Iijima M, Okamoto Y, Misaki R, Fujiyama K, Nagamori S, Kanai Y, Takehara T, Ueda K, Kuroda S, Miyoshi E.
  • Journal Title: Glycobiology. Volume: 26 Pages: 1180-1189
  • DOI: 10.1093/glycob/cww067
  • Peer Reviewed
• [Journal Article] Cellular uptake of hepatitis B virus envelope L particles is independent of sodium taurocholate cotransporting polypeptide, but dependent on heparan sulfate proteoglycan (2016)
  • Author(s): Somiya M, Liu Q, Yoshimoto N, Iijima M, Tatematsu K, Nakai T, Okajima T, Kuroki K, Ueda K, Kuroda S.
  • Journal Title: Virology Volume: 497 Pages: 23-32
  • DOI: 10.1016/j.virol.2016.06.024
  • Peer Reviewed
• [Journal Article] High efficiency penetration of antibody-immobilized nanoneedle thorough plasma membrane for in situ detection of cytoskeletal proteins in living cells (2016)
  • Author(s): R. Kawamura, K. Shimizu, Y. Matsumoto, A. Yamagishi, Y. R. Silberberg, M. Iijima, S. Kuroda, K. Fukazawa, K. Ishihara and C. Nakamura
  • Journal Title: Journal of Nanobiotechnology Volume: 14 Issue: 1 Pages: 74-82
  • DOI: 10.1186/s12951-016-0226-5
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] バイオナノカプセル足場技術を用いた食物アレルゲンの簡便・迅速・高感度検出法の開発 (2020)
  • Author(s): 山本 星里、藤方 楓、黒田 俊一、中山 勉、飯嶋 益巳
  • Organizer: 日本農芸化学会2020年度大会
• [Presentation] バイオナノカプセル足場技術を用いた食品検査キットの高感度化 (2020)
  • Author(s): 水野 麻美、黒田 俊一、中山 勉、飯嶋 益巳
  • Organizer: 日本農芸化学会2020年度大会
• [Presentation] Analysis of mechanical property of intermediate filament in a living cell using antibody-functionalized nanoneedle and AFM (2019)
  • Author(s): Susaki M., Yamagishi A., Iijima M., Kuroda S., Nakamura C.
  • Organizer: 11th EUROPEAN MEETING ON INTERMEDIATE FILAMENTS AND COST ACTION EUROCELLNET MEETING
  • Int'l Joint Research
• [Presentation] Bio-nanocapsule-based scaffolding technology for clustering and oriented immobilization of various sensing molecules on biosensor surface (2019)
  • Author(s): Iijima M., Nakayama T., Kuroda S.
  • Organizer: 6th International Conference on Bio-Sensing Technology
  • Int'l Joint Research
• [Presentation] ナノニードルを用いた繰り返し引っ張り試験による中間径フィラメントの可動性解析 (2019)
  • Author(s): 中村 史, 須﨑 萌, 水澤愛衣, 山岸彩奈, 飯嶋益巳, 黒田俊一
  • Organizer: 第71回日本生物工学会大会
• [Presentation] センシング分子ナノ整列化技術を用いた食品分析の高感度化 (2019)
  • Author(s): 飯嶋益巳
  • Organizer: 日本食品分析学会 令和元年度総会・学術集会
  • Invited
• [Presentation] Mechanism of affinity enhanced protein adsorption on bio-nanocapsules studied by viscoelasticity measurement with wireless QCM biosensor (2019)
  • Author(s): Noi K., Iijima M., Kuroda S., Ogi H.
  • Organizer: 第40回超音波エレクトロニクスの基礎と応用に関するシンポジウム
• [Presentation] Bio-nanocapsule-based scaffolding technology: Oriented immobilization and clustering of Fc-fused lectins and DNA aptamers on biosensor surface (2018)
  • Author(s): Iijima, M., Yamada, Y., Kuroda S.
  • Organizer: Biosensors2018
  • Int'l Joint Research
• [Presentation] Discrimination and mechanical separation of neural stem cell derived from human iPS cell using antibody-functionalized nanoneedle array (2018)
  • Author(s): Matsumoto, Y., Kawamura, R., Yamagishi, A., Iijima, M., Kuroda, S., Nakamura, C.
  • Organizer: Biosensors2018
  • Int'l Joint Research
• [Presentation] Bio-nanocapsule-based Scaffolds for Biosensing Techniques: A Clustering and Oriented Immobilization of Sensing Molecules (2018)
  • Author(s): Yamada, Y., Iijima, M., Kuroda, S.
  • Organizer: The First International Joint Symposium of CEFMS-NCTU, RCAS-AS (Taiwan) and 5-Star Alliance (Japan)
  • Int'l Joint Research
• [Presentation] バイオナノカプセル整列固定化技術によるDNAアプタマーセンサーの高感度化 (2018)
  • Author(s): 山田裕紀、飯嶋益巳、黒田俊一
  • Organizer: 第70回日本生物工学会大会
• [Presentation] バイオセンサ表層におけるセンシング分子精密整列化技術の開発 (2018)
  • Author(s): 飯嶋益巳
  • Organizer: 応用物理学会 有機分子・バイオエレクトロニクス分科会講習会
  • Invited
• [Presentation] Bio-nanocapsule-based scaffold for clustering and oriented-immobilization of sensing molecules (2018)
  • Author(s): Yamada, Y., Iijima, M., Kuroda S.
  • Organizer: 21st SANKEN International Symposium
  • Int'l Joint Research
• [Presentation] DNAアプタマーを整列固定化するバイオナノカプセル足場分子の開発 (2018)
  • Author(s): 山田裕紀, 飯嶋益巳, 黒田俊一
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] 抗体修飾ナノニードルアレイを用いた機械的細胞分離 (2018)
  • Author(s): 松本雄太, 川村 隆三, 山岸彩奈, 飯嶋益巳, 黒田俊一, 中村 史
  • Organizer: 第65回応用物理学会春季学術講演会
• [Presentation] ナノニードルアレイを用いたヒトiPS細胞由来神経幹細胞の機械的細胞分離 (2018)
  • Author(s): 松本雄太, 川村 隆三, 山岸彩奈, 飯嶋益巳, 黒田俊一, 中村 史
  • Organizer: 日本化学会第98春季年会（2018）
• [Presentation] 抗体を整列固定化する平面膜の開発 (2017)
  • Author(s): 飯嶋益巳、黒田俊一
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-17
• [Presentation] バイオセンサー表層におけるセンシング分子のナノレベル精密整列化に関する研究 (2017)
  • Author(s): 飯嶋益巳
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都
  • Year and Date: 2017-03-17
  • Invited
• [Presentation] ナノニードルアレイを用いた機械的細胞分離技術の開発 (2017)
  • Author(s): 松本雄太、飯嶋益巳、黒田俊一、山岸彩奈、中村 史
  • Organizer: 日本化学会第97春季年会（2017）
  • Place of Presentation: 神奈川
  • Year and Date: 2017-03-16
• [Presentation] 細胞骨格の機械的特性の解析に向けた中間径フィラメントの引張試験 (2017)
  • Author(s): 須崎 萌、竹田 至、山岸 彩奈、飯嶋 益巳、黒田 俊一、岡田 知子、金 賢徹、中村 史
  • Organizer: 第64回応用物理学会春季学術講演会
  • Place of Presentation: 横浜
  • Year and Date: 2017-03-14
• [Presentation] Planar membrane displaying IgGs in an oriented immobilization manner for biosensor surface (2017)
  • Author(s): Iijima, M., Kuroda S.
  • Organizer: 5th International Conference on Bio-Sensing Technology
  • Int'l Joint Research
• [Presentation] Capsular- and Planar-Scaffold for Clustering and Oriented Immobilization of Sensing Molecules (2017)
  • Author(s): Iijima, M., Kuroda S.
  • Organizer: Nanotech France 2017 Conference and Exhibition
  • Int'l Joint Research
• [Presentation] ヒトiPS由来神経幹細胞の機械的細胞分離 (2017)
  • Author(s): 松本雄太, 清水桂太, 川村 隆三, 山岸彩奈, 飯嶋益巳, 黒田俊一, 中村 史
  • Organizer: 第11回バイオ関連化学シンポジウム
• [Presentation] Development of scaffolding molecule for improving function of biomolecules (2017)
  • Author(s): Iijima, M., Kuroda S.
  • Organizer: 42nd FEBS Congress
  • Int'l Joint Research
• [Presentation] Mechanical Separation of Neural Stem Cell Derived from Human iPS Cell Using Nanoneedle Array (2017)
  • Author(s): Matsumoto, Y., Shimizu, K., Kawamura, R., Yamagishi, A., Iijima, M., Kuroda, S., Nakamura, C.
  • Organizer: IGER International Symposium on Cell Surface Structures and Functions 2017
  • Int'l Joint Research
• [Presentation] Evaluation of mechanical property of intermediate filament related with stiffness of breast cancer cell by use of nanoneedle and AFM (2017)
  • Author(s): Yamagishi, A., Susaki, M., Takano, U., Iijima, M., Kuroda, S., Okada, T., Nagasaki, A., Nakamura, C.
  • Organizer: The 2017 MRS Fall Meeting
  • Int'l Joint Research
• [Presentation] バイオセンサー表層におけるセンシング分子のナノレベル精密整列化に関する研究 (2017)
  • Author(s): 飯嶋益巳
  • Organizer: 日本農芸化学会関西支部例会（第501回講演会）
  • Invited
• [Presentation] ナノ界面における抗体分子整列化技術および医療診断への応用 (2017)
  • Author(s): 飯嶋益巳
  • Organizer: 2017 Promega Dynamic Connection
  • Invited
• [Presentation] Reconstituted planar ZZ-L membrane for the oriented immobilization of immunoglobulin G on biosensors (2016)
  • Author(s): Iijima, M., Kuroda S.
  • Organizer: The 20th SANKEN International The 15th SANKEN Nanotechnology Symposium, The Institute of Scientific and Industrial Research (ISIR)
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2016-12-12
  • Int'l Joint Research
• [Presentation] Synthesis and assembly of hepatitis B virus envelope protein-derived particles in Escherichia coli (2016)
  • Author(s): Li, H., Onbe, K., Liu, Q., Iijima, M., Yoshimoto, N., Nakai, T., Tatematsu, K., Okajima, T., Seno, M., H, Tada., Kuroda, S.
  • Organizer: The 20th SANKEN International The 15th SANKEN Nanotechnology Symposium, The Institute of Scientific and Industrial Research (ISIR)
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2016-12-12
  • Int'l Joint Research
• [Presentation] High efficiency insertion of antibody-immobilized nanoneedle into living cells for in situ detection of cytoskeletal proteins (2016)
  • Author(s): Nakamura, C., Shimizu, K., Kawamura, R., Iijima, M., Kuroda, S., Fukazawa, K., Ishihara, K.
  • Organizer: PRiME2016
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2016-10-02
  • Int'l Joint Research
• [Presentation] センシング分子を最密充填状態で精密に整列固定化する二次元膜の開発 (2016)
  • Author(s): 飯嶋益巳、黒田俊一
  • Organizer: 第68回日本生物工学会大会
  • Place of Presentation: 富山
  • Year and Date: 2016-09-28
• [Presentation] ナノニードルアレイを用いたビメンチン陽性癌細胞分離技術の開発 (2016)
  • Author(s): 松本雄太、川村隆三、飯嶋益巳、黒田俊一、中村 史
  • Organizer: 第68回日本生物工学会大会
  • Place of Presentation: 富山
  • Year and Date: 2016-09-28
• [Presentation] 様々な抗体を提示可能なバイオナノカプセルによる能動的標的化DDSナノキャリアの開発 (2016)
  • Author(s): 立松健司、李昊、飯嶋益巳、黒田俊一
  • Organizer: 第68回日本生物工学会大会
  • Place of Presentation: 富山
  • Year and Date: 2016-09-28
• [Presentation] Reconstituted ZZ-L particles and planar ZZ-L membrane for the oriented immobilization of Fc-fused molecules on various types of biosensors. (2016)
  • Author(s): Iijima, M., Kuroda S.
  • Organizer: Biosensors2016
  • Place of Presentation: Gothenburg, Sweden
  • Year and Date: 2016-05-25
  • Int'l Joint Research
• [Presentation] High efficiency penetration of antibody-immobilized nanoneedle thorough plasma membrane for in situ detection of cytoskeletal proteins in living cells (2016)
  • Author(s): Shimizu, K., Kawamura, R., Iijima, M., Kuroda, S., Fukazawa, K., Ishihara, K., Nakamura, C.
  • Organizer: Biosensors2016
  • Place of Presentation: Gothenburg, Sweden
  • Year and Date: 2016-05-25
  • Int'l Joint Research
• [Book] 細胞・生体分子の固定化と機能発現 第1編 第13章 バイオナノカプセル足場分子を用いた抗体の精密整列固定化とバイオセンシング技術への応用 (2018)
  • Author(s): 飯嶋益巳、黒田俊一
  • Total Pages: 294
  • Publisher: CMC出版
  • ISBN: 9784781313269
• [Book] 飯嶋益巳、黒田俊一 (2018)
  • Author(s): 化学と生物
  • Total Pages: 60
  • Publisher: 国際文献社
• [Book] ケミカルエンジニアリング，特集＝センシング技術の話題 (2017)
  • Author(s): 飯嶋益巳, 黒田俊一
  • Total Pages: 80
  • Publisher: 化学工業社
• [Remarks] ZZ-BNCを用いた生体分子のナノレベル整列固定化技術
  • URL: http://www.sanken.osaka-u.ac.jp/labs/smb/index.html
• [Patent(Industrial Property Rights)] 医薬 (2017)
  • Inventor(s): 黒田俊一、飯嶋益巳、立松健司
  • Industrial Property Rights Holder: 黒田俊一、飯嶋益巳、立松健司
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017-03-21