High-resolution imaging of intermolecular interactions undergoing mechanical modulation

• Project/Area Number: 16K04908
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nanobioscience
• Research Institution: Kindai University (2018-2019); Kyoto University (2016-2017)
• Principal Investigator: Nishiyama Masayoshi 近畿大学, 理工学部, 准教授 (10346075)
• Co-Investigator(Kenkyū-buntansha): 森松 賢順 岡山大学, 医歯薬学総合研究科, 助教 (70580934)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 高圧力顕微鏡 / 水和 / 分子間相互作用 / 分子機械 / 細胞活動の活性化 / 細胞骨格

Outline of Final Research Achievements

Application of hydrostatic pressure is a powerful method for modulating intermolecular interactions between protein and water molecules. We have developed a high-pressure microscope that is optimized both for the best image formation and for the stability to hydrostatic pressure up to 150 MPa. The developed apparatus allows us to visualize the structural and functional changes of molecular machines. By using high-pressure microscopy, we have developed a novel technique that controls the molecular machines working in living cells. The developed technique successfully demonstrated that application of pressures can increase the activity in living cells.

Academic Significance and Societal Importance of the Research Achievements

これまでの生命科学分野では、遺伝子操作や化学物質を添加することで、細胞内での生命活動を操作する研究が実施されてきた。本研究で開発した技術は、これらの既存の技術とは異なり、静水圧という「力」を用いた新しい細胞操作技術である。今後も研究を継続し、圧力による活性化を通じて、新しい医療技術の芽を育むことができるように努めていく。

Research Products

• [Journal Article] High hydrostatic pressure induces vigorous flagellar beating in Chlamydomonas non-motile mutants lacking the central apparatus (2020)
  • Author(s): Yagi T. & Nishiyama M.
  • Journal Title: Scientific Reports Volume: 10 Issue: 1
  • DOI: 10.1038/s41598-020-58832-8
  • NAID: 120006799256
  • Peer Reviewed / Open Access
• [Journal Article] Increased hydrostatic pressure induces nuclear translocation of DAF-16/FOXO in C.elegans (2020)
  • Author(s): Watanabe Naoshi、Morimatsu Masatoshi、Fujita Ayano、Teranishi Mika、Sudevan Surabhi、Watanabe Masaru、Iwasa Hiroaki、Hata Yutaka、Kagi Hiroyuki、Nishiyama Masayoshi、Naruse Keiji、Higashitani Atsushi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 523 Issue: 4 Pages: 853-858
  • DOI: 10.1016/j.bbrc.2020.01.047
  • Peer Reviewed / Open Access
• [Journal Article] High pressure inhibits signaling protein binding to the flagellar motor and bacterial chemotaxis through enhanced hydration (2020)
  • Author(s): Hata H., Nishihara Y., Nishiyama M., Sowa Y., Kawagishi I. & Kitao A.
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 2351-2351
  • DOI: 10.1038/s41598-020-59172-3
  • NAID: 120006811771
  • Peer Reviewed / Open Access
• [Journal Article] Session 1SHA-control of biological functions with hydrostatic pressure stimulation. (2020)
  • Author(s): Hata H. & Nishiyama M.
  • Journal Title: Biophysical Reviews Volume: - Issue: 2 Pages: 269-270
  • DOI: 10.1007/s12551-020-00658-9
  • Peer Reviewed
• [Journal Article] Molecular dynamics simulation of proteins under high pressure: Structure, function and thermodynamics (2020)
  • Author(s): Hata Hiroaki、Nishiyama Masayoshi、Kitao Akio
  • Journal Title: Biochimica et Biophysica Acta (BBA) - General Subjects Volume: 1864 Issue: 2 Pages: 129395-129395
  • DOI: 10.1016/j.bbagen.2019.07.004
  • Peer Reviewed
• [Journal Article] Controlling the Motility of ATP-Driven Molecular Motors Using High Hydrostatic Pressure (2018)
  • Author(s): Nishiyama Masayoshi
  • Journal Title: The Role of Water in ATP Hydrolysis Energy Transduction Volume: - Pages: 325-337
  • DOI: 10.1007/978-981-10-8459-1_19
  • ISBN: 9789811084584, 9789811084591
  • Peer Reviewed
• [Journal Article] 細菌の祖先がもつ運動マシナリーを現代に蘇らせる (2018)
  • Author(s): 西山雅祥, 金井保, 竹川宣宏
  • Journal Title: 生物工学会誌 Volume: 96 Pages: 11-14
  • Open Access
• [Journal Article] Commonly stabilized cytochromes c from deep-sea Shewanella and Pseudomonas (2018)
  • Author(s): Fujii Sotaro、Masanari-Fujii Misa、Kobayashi Shinya、Kato Chiaki、Nishiyama Masayoshi、Harada Yoshie、Wakai Satoshi、Sambongi Yoshihiro
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 82 Issue: 5 Pages: 792-799
  • DOI: 10.1080/09168451.2018.1448255
  • Peer Reviewed
• [Journal Article] Morphological Control of Microtubule-Encapsulating Giant Vesicles by Changing Hydrostatic Pressure (2018)
  • Author(s): Takiguchi K., Hayashi M., Kazayama Y., Toyota T., Harada Y. & Nishiyama M.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 41 Issue: 3 Pages: 288-293
  • DOI: 10.1248/bpb.b17-00366
  • NAID: 130006407352
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access
• [Journal Article] Tracking the Movement of a Single Prokaryotic Cell in Extreme Environmental Conditions. (2017)
  • Author(s): Nishiyama M. & Arai Y.
  • Journal Title: Methods in Molecular Biology Volume: 1593 Pages: 175-184
  • DOI: 10.1007/978-1-4939-6927-2_13
  • ISBN: 9781493969265, 9781493969272
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] High-pressure microscopy for tracking dynamic properties. of molecular machines. (2017)
  • Author(s): Nishiyama M.
  • Journal Title: Biophysical Chemistry Volume: 印刷中 Pages: 71-78
  • DOI: 10.1016/j.bpc.2017.03.010
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 高圧力顕微鏡法による微小管のダイナミックコントロール (2016)
  • Author(s): 西山雅祥, 瀧口金吾, 林真人
  • Journal Title: 顕微鏡 Volume: 51 Pages: 118-121
  • NAID: 130007695967
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Activation of the motility machineries using high-pressure techniques. (2019)
  • Author(s): Nishiyama M.
  • Organizer: 第57回日本生物物理学会年会
  • Invited
• [Presentation] 静水圧を力学刺激とする生命操作技術の開発 (2019)
  • Author(s): 西山 雅祥
  • Organizer: 日本メカノバイオロジー研究会
  • Invited
• [Presentation] Pressure-induced activation of the cell motility (2018)
  • Author(s): Nishiyama M.
  • Organizer: 第56回日本生物物理学会年会
  • Invited
• [Presentation] High hydrostatic pressure induces cardiomyocyte contraction (2018)
  • Author(s): 山口陽平, 西山 雅祥, 甲斐 寛彬, 入部玄太郎, 成瀬恵治, 森松 賢順
  • Organizer: 第56回日本生物物理学会年会
• [Presentation] Mechanical stress modulates the homeostasis of periodontal ligament (2018)
  • Author(s): 藤田 彩乃, 森松賢順, 西山雅祥, 高柴正悟, 成瀬恵治
  • Organizer: 第56回日本生物物理学会年会
• [Presentation] Viewing the rhythmical beating motion of paralyzed-flagella mutants of Chlamydomonas at high-pressure (2018)
  • Author(s): Nishiyama M., Yagi T.
  • Organizer: HPBB2018
  • Int'l Joint Research
• [Presentation] High-pressure microscopy for controlling molecular machines in living cells. (2018)
  • Author(s): Nishiyama M.
  • Organizer: International Congress on Pure & Applied Chemistry (ICPAC) 2018, Siem Reap, Cambodia
  • Int'l Joint Research / Invited
• [Presentation] 咀嚼時に高圧力が負荷されるヒト由来歯根膜細胞を可視化する (2018)
  • Author(s): 藤田彩乃, 森松賢順, 西山雅祥，高柴正悟, 成瀬恵治
  • Organizer: 2018年 生体運動研究合同班会議
• [Presentation] EFFECTS OF MECHANICAL STRESS ON PERIODONTAL LIGAMENT (2018)
  • Author(s): Fujita A., Morimatsu, M., Nishiyama M., Takashiba S., Naruse K.
  • Organizer: 62nd Annual Meeting of Biophysical Society, San Francisco, USA
  • Int'l Joint Research
• [Presentation] REVERSIBLE MORPHOLOGICAL CONTROL OF TUBULIN-ENCAPSULATING GIANT LIPOSOMES BY HYDROSTATIC PRESSURE (2017)
  • Author(s): Nishiyama M, Hayashi M., Takiguchi K. & Harada Y.
  • Organizer: 61st Annual Meeting of Biophysical Society
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2017-02-11
  • Int'l Joint Research
• [Presentation] 静水圧を用いた細胞活動の活性化イメージング (2017)
  • Author(s): 西山 雅祥
  • Organizer: 同志社大
• [Presentation] ヒト由来歯根膜細胞の力学刺激応答イメージング (2017)
  • Author(s): 藤田彩乃, 森松賢順, 西山雅祥，高柴正悟, 成瀬恵治
  • Organizer: 第78回 応用物理学会秋季学術講演会
• [Presentation] メカノストレスによる歯周組織リモデリング機構の解明 (2017)
  • Author(s): 藤田彩乃, 森松賢順, 西山雅祥，高柴正悟, 成瀬恵治
  • Organizer: 第55回 日本生物物理学会年会
• [Presentation] 高圧力顕微鏡を用いたヒト由来歯根膜細胞の実時間観察 (2017)
  • Author(s): 藤田彩乃, 森松賢順, 西山雅祥，高柴正悟, 成瀬恵治
  • Organizer: 特殊環境微生物セミナー2017
• [Presentation] 高圧力顕微鏡法を用いたヒト由来歯根膜細胞の動態イメージング (2017)
  • Author(s): 藤田彩乃, 森松賢順, 西山雅祥，高柴正悟, 成瀬恵治
  • Organizer: 第58回高圧討論会
• [Presentation] 生きた細胞内で働く分子機械を高圧力顕微鏡法でみる (2016)
  • Author(s): 西山雅祥
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜, 横浜
  • Year and Date: 2016-11-30
  • Invited
• [Presentation] High-pressure microscopy for controlling molecular machines in living cells. (2016)
  • Author(s): Nishiyama M.
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場, 筑波
  • Year and Date: 2016-11-25
  • Invited
• [Presentation] 高静水圧環境の分子ダイナミクスを直接みる (2016)
  • Author(s): 西山雅祥
  • Organizer: 第59回日本顕微鏡学会シンポジウム
  • Place of Presentation: 帝京大学
  • Year and Date: 2016-11-18
  • Invited
• [Presentation] Microscopic analysis of the swimming motility of deep-sea bacteria at high pressure. (2016)
  • Author(s): Nishiyama M., Kato c., Imai H.
  • Organizer: 5th International Workshop on Deep-Sea Microbiology, Kyoto
  • Place of Presentation: 京都大学, 京都
  • Year and Date: 2016-09-10
  • Int'l Joint Research / Invited
• [Presentation] High-pressure microscopy for controlling the dynamics of microtubule cytoskeleton (2016)
  • Author(s): Nishiyama M.
  • Organizer: 9th International Conference on High Pressure Bioscience and Biotechnology
  • Place of Presentation: Tronto University, Toronto, Canada
  • Year and Date: 2016-07-25
  • Int'l Joint Research / Invited
• [Presentation] 高圧力顕微鏡を用いた深海微生物の運動観察 (2016)
  • Author(s): 西山雅祥
  • Organizer: 第6回分子モーター討論会
  • Place of Presentation: 大阪大学, 大阪
  • Year and Date: 2016-07-24
  • Invited
• [Presentation] 高圧力顕微鏡法による細胞構造コントロール (2016)
  • Author(s): 西山雅祥
  • Organizer: 第55回日本生体医工学会大会
  • Place of Presentation: 富山国際会議場, 富山
  • Year and Date: 2016-04-26
  • Invited
• [Presentation] 高圧力顕微鏡を使った生体運動イメージング (2016)
  • Author(s): 西山雅祥
  • Organizer: Bio-Divセミナー
  • Place of Presentation: JAMSTEC, 横浜
  • Invited
• [Book] 高圧力顕微鏡法によるタンパク質分子機械の回転制御 (2017)
  • Author(s): 西山雅祥
  • Publisher: 養賢堂
• [Remarks] 失った細胞機能を水圧で再生 ヒト精子の不妊治療など医療技術に応用できる可能性も
  • URL: http://www.news2u.net/releases/167989