| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The aim of the present study is to elucidate the mechanism of dynamic instability of microtubules through investigation of their behaviors inside femtoliter chambers. Tubulins were encapsulated in the arrays of cylindrical microchambers patterned on PDMS devices, and polymerization and depolymerization reactions were observed under the optical microscope. The rate of polymerization in this confined space was apparently higher than that in the space that is 1000 times larger in volume. After reaching to the barrier of PDMS walls, flexible microtubules grew in curved shape along the wall supposedly incorporating lattice defects in the structure. In this phase, the rate of polymerization decreased depending on the curvature of the microtubule. These results seem to be consistent with conformational plasticity model of microtubules.
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