Control of Nano-structure Surface for Highly Sensitive Biosensors
| Project/Area Number |
16K04928
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied materials
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
OHNUKI Hitoshi 東京海洋大学, 学術研究院, 教授 (60223898)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | バイオセンサ / 免疫センサ / 抗原抗体反応 / 配向制御 / 電気化学インピーダンス法 / ミオグロビン / 心筋梗塞 / 再生技術 / 抗体配向効果 |
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| Outline of Final Research Achievements |
We developed novel biosensors which have antibody oriented surface together with regenerable feature. We employed Protein G as a receptor. The Protein G selectively binds to the IgG type antibody with keeping a fixed molecular direction under a neutral pH condition, but it cuts the bind under a low pH condition. Accordingly, the antibodies captured by the Protein G are oriented on the surface, exposed their sensing portion to the outside.
In this study, the anti-myoglobin antibodies were immobilized on Protein Gs to form a myoglobin biosensor. The obtained myoglobin biosensor showed 3 times larger intensity and the regenerable property.
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| Academic Significance and Societal Importance of the Research Achievements |
心筋梗塞は発症後すぐに病院で治療を受けた際の死亡率が 10 % 以下だと言われている．つまり，心筋梗塞による死者を減らすには早期発見が非常に重要となる．この早期発見において注目されている物質がミオグロビンである．本研究では，ミオグロビン等の疾病の進行に伴い急増するバイオマーカーを，高感度でその場測定する手法としてバイオセンサの開発を行った．さらに，本研究で開発されたセンサの再生技術を組み合わせれば，1回計測あたりの経費節減とセンサ作製時間の短縮が可能となる．このような高感度バイオセンサは，将来の予防医療分野の発展を促進し，健康福祉社会の実現に大きく貢献できると考える．
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Report
(4 results)
Research Products
(19 results)
