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Development of single-microdroplet microscope using quantum nature of light and its application to biomoleculer fluctuation

Research Project

Project/Area Number 16K05661
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Physical chemistry
Research InstitutionOsaka City University

Principal Investigator

Sakota Kenji  大阪市立大学, 大学院理学研究科, 准教授 (80346767)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords微小液滴 / イオントラップ / 両親媒性分子 / 励起エネルギー移動 / シアニン色素 / ゆらぎ / 顕微分光 / 単一分子分光
Outline of Final Research Achievements

A single micro droplet can be regarded as a tiny optical resonator. By utilizing the amplification of the light generated in the optical resonator, it becomes possible to detect the molecule dissolved in the droplet with high sensitivity. In this study, we succeeded in the detection of the molecule dissolved in the droplet at about 80 times sensitivity in comparison with the previously-reported equipment by using a home-built three-dimensional ion trap and microscope. In addition, it succeeded in quantitatively measuring the excitation light resonance which the excitation light irradiated to the droplet shows in wide size region. As one of the model biomembrane research, we observed the energy transfer between amphiphilic molecules which were adsorbed on the air-liquid interface in the droplet. It was found that the energy transfer proceeds efficiently in the droplet in comparison with the bulk solution.

Academic Significance and Societal Importance of the Research Achievements

近年,微小液滴のようなミクロな空間で進行する化学反応は,より大きなスケール(バルク溶液)で進む化学反応とは,本質的な違いがあることが明らかになりつつある.バルク溶液では殆ど進行しない化学反応が,微小液滴では効率的に進行する例も報告されている.本研究によって,微小液滴に溶存した分子を高感度に検出することが可能となった.この方法を応用すれば,バルク溶液と微小液滴での化学反応機構の違いを明らかにできる可能性があり,将来的には,バルク溶液では合成することが困難な薬のような有用物質を微小液滴内で合成することが可能になるなど,創薬分野への波及効果も期待できる.

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (27 results)

All 2019 2018 2017 2016

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (24 results) (of which Int'l Joint Research: 9 results,  Invited: 9 results)

  • [Journal Article] Synthesis of Bare Iron Nanoparticles from Ferrocene Hexane Solution by Femtosecond Laser Pulses2018

    • Author(s)
      T. Okamoto, T. Nakamura, R. Kihara, T. Asahi, K. Sakota, T. Yatsuhashi
    • Journal Title

      ChemPhysChem

      Volume: 19 Issue: 19 Pages: 2480-2485

    • DOI

      10.1002/cphc.201800436

    • NAID

      120006734420

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2019763

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Real-time observation of the photoionization-induced water rearrangement dynamics in the 5-hydroxyindole-water cluster by time-resolved IR spectroscopy2018

    • Author(s)
      Mitsuhiko Miyazaki, Ayumi Naito, Takamasa Ikeda, Johanna Klyne, Kenji Sakota, Hiroshi Sekiya, Otto Dopfer, Masaaki Fujii
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 20 Issue: 5 Pages: 3079-3091

    • DOI

      10.1039/c7cp06127g

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Elevation of the Energy Threshold for Isomerization of 5-Hydroxyindole-(tert-butyl alcohol)1 Cluster Cations2017

    • Author(s)
      Takamasa Ikeda, Kenji Sakota, Hiroshi Sekiya
    • Journal Title

      J. Phys. Chem. A

      Volume: 121 Issue: 31 Pages: 5809-5816

    • DOI

      10.1021/acs.jpca.7b05434

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Fluorescence enhancement caused by the excitation light confinement in a single microdroplet2019

    • Author(s)
      Kenji Sakota
    • Organizer
      Manchester International Symposium: Highly-excited States and Non-covalent Interactions
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The cavity mode dependent confinement of excitation light in a single levitated microdroplet2019

    • Author(s)
      Kenji Sakota
    • Organizer
      The 23th East Asian Workshop on Chemical Dynamics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A single droplet microscopy for highly sensitive fluorescence detection2019

    • Author(s)
      Kenji Sakota
    • Organizer
      WRHI International Workshop
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Microscopy of a single levitated microdroplet for biomolecular applications2019

    • Author(s)
      Kenji Sakota
    • Organizer
      13th International Symposium on Nanomedicine (ISNM2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 単一微小液滴で生じる励起光の閉じ込めに起因した蛍光増強とその液滴径依存性2019

    • Author(s)
      佐野元哉,八ッ橋知幸,迫田憲治
    • Organizer
      第13回分子科学討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 平行平板型イオントラップとエンドキャップトラップの性能比較2019

    • Author(s)
      野口昌起,佐野元哉,八ッ橋知幸,迫田憲治
    • Organizer
      第13回分子科学討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Performance comparison with a parallel plate iontrap and an endcap iontrap2019

    • Author(s)
      Masaki Noguchi, Motoya Sano, Tomoyuki Yatsuhashi, Kenji Sakota
    • Organizer
      International Workshop on Quantum Sensing & Biophotonics 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 両親媒性シアニン色素の繊維状J会合体形成に関する分光学的研究2019

    • Author(s)
      山本駿介,八ッ橋知幸,迫田憲治
    • Organizer
      第99回日本化学会春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] J-aggregate formation of amphiphilic cyanine dyes in alcohol-water mixed solutions2018

    • Author(s)
      Shunsuke Yamamoto, Hiroyuki Miyake, Tomoyuki Yatsuhashi, Kenji Sakota
    • Organizer
      34th Symposium on Chemical Kinetics and Dynamics
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] メタノール/水混合溶液中における両親媒性シアニン色素のファイバー状J会合体形成2018

    • Author(s)
      山本駿介,三宅弘之,津田明彦,八ッ橋知幸,迫田憲治
    • Organizer
      第12回分子科学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] Laser chemistry for Eu recovery2018

    • Author(s)
      Nobuaki Nakashima, Tomoyuki Yatsuhashi, Kenji Sakota, Izumi Iwakura, Keiichi Yokoyama
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 空間捕捉した単一微小液滴表面におけるシアニン色素のレーザー顕微分光2017

    • Author(s)
      大谷拓也,迫田憲治
    • Organizer
      日本化学会年会
    • Place of Presentation
      慶應大学(神奈川県・横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Laser spectroscopy of a single levitated microdroplet ~Lasing and resonance energy transfer~2017

    • Author(s)
      Kenji Sakota
    • Organizer
      International Symposium on "Diversity of Chemical Reaction Dynamics"
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 単一微小液滴の顕微分光とバルク溶液中におけるキラルJ会合体の研究2017

    • Author(s)
      迫田憲治
    • Organizer
      第40回溶液化学シンポジウム
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 空間捕捉した単一微小液滴のレーザー顕微分光2017

    • Author(s)
      安冨翔太,佐野元哉,山本駿介,大谷拓也,関谷博,八ツ橋知幸,迫田憲治
    • Organizer
      第11回分子科学討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] 両親媒性シアニン色素のキラルJ会合体形成における混合溶媒の組成比依存性2017

    • Author(s)
      大谷拓也,三宅弘之,八ツ橋知幸,迫田憲治
    • Organizer
      第40回溶液化学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 分光測定から観た分子科学-真空中から細胞模倣環境までー2016

    • Author(s)
      迫田憲治
    • Organizer
      第17回大つくば物理化学セミナー
    • Place of Presentation
      城西大学鋸南セミナーハウス(千葉県・安房郡鋸南町)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 空間捕捉した単一微小液滴の表面に構築した単分子膜のレーザー顕微分光2016

    • Author(s)
      安冨翔太,迫田憲治
    • Organizer
      第39回溶液化学シンポジウム
    • Place of Presentation
      産業技術総合研究所(茨城県・つくば市)
    • Year and Date
      2016-11-09
    • Related Report
      2016 Research-status Report
  • [Presentation] 空間捕捉した単一微小液滴表面に構築した生体膜のレーザー顕微分光2016

    • Author(s)
      安冨翔太,迫田憲治
    • Organizer
      第10回分子科学討論会
    • Place of Presentation
      神戸ファッションマート(兵庫県・神戸市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒドロキシプロピルセルロースの構造転移に対するホフマイスターアニオンおよびポリエチレングリコールの複合効果2016

    • Author(s)
      米山可凛,関谷博,迫田憲治
    • Organizer
      第10回分子科学討論会
    • Place of Presentation
      神戸ファッションマート(兵庫県・神戸市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Research-status Report
  • [Presentation] リボヌクレアーゼAの安定性に対する電解質と分子混み合いの複合効果2016

    • Author(s)
      大内仁,関谷博,迫田憲治
    • Organizer
      第10回分子科学討論会
    • Place of Presentation
      神戸ファッションマート(兵庫県・神戸市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Research-status Report
  • [Presentation] The Influence of Molecular-weight Specific Adsorption of Polyethylene Glycol on Two-state Transition of Myoglobin2016

    • Author(s)
      Shota Yasutomi, Kenji Sakota, Hiroshi Sekiya
    • Organizer
      Recent Progress in Molecular Spectroscopy and Dynamics
    • Place of Presentation
      Kyushu University(福岡県・福岡市)
    • Year and Date
      2016-07-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Laser Microscopy on Biomolecules Dissolved in a Levitated Single Microdroplet2016

    • Author(s)
      Kenji Sakota
    • Organizer
      20th East Asia Workshop on Chemical Dynamics
    • Place of Presentation
      National Kaohsiung University (Taiwan)
    • Year and Date
      2016-06-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 生体膜研究への応用を目指した単一微小液滴のレーザー顕微分光2016

    • Author(s)
      迫田憲治
    • Organizer
      研究会「分子を使った寄木細工」~自己組織化したソフトマテリアルが織りなす「かたち」と機能~
    • Place of Presentation
      島根大学(島根県・松江市)
    • Year and Date
      2016-05-27
    • Related Report
      2016 Research-status Report
    • Invited

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Published: 2016-04-21   Modified: 2021-02-19  

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