Colorimetric Sensing of Trace Amounts of Albumin/Amino Acids using Surface-Enhanced Photochromic Phenomena
| Project/Area Number |
16K05817
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Yamaguchi University |
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Principal Investigator |
Adachi Kenta 山口大学, 大学院創成科学研究科, 准教授 (80535245)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | タンパク質 / アミノ酸 / ナノ粒子 / フォトクロミズム / 吸着 / 比色分析 / ポリオキソメタレート / 分析化学 / アルブミン / センシング |
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| Outline of Final Research Achievements |
Due to the biological importance of α-amino acids and their derivatives, the development of novel colorimetric probes for these molecules has been an active research area in recent years. In this study, the surface-enhanced photochromic phenomena by various α-amino acids adsorbed on molybdenum(VI) oxide (MoO3) nanoparticles in the aqueous system have been investigated by means of UV-vis absorption spectrometry. No derivatization of α-amino acids is required, and eventually high-accuracy determination is obtained from the UV-induced MoO3 photochromic coloration. During UV irradiation, the detection process could be visually observed by naked eyes. A simple and unique approach to explore sensitive "label-free" colorimetric sensing toward α-amino acids is proposed. Additionally, human serum albumin was added to tungsten(VI) oxide (WO3) and/or MoO3 nanoparticle aqueous solution to probe the photochromic properties.
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| Academic Significance and Societal Importance of the Research Achievements |
昨今の急速な高齢化社会の進展と生活習慣病の増加を鑑みるに『生体試料中のタンパク質、そしてその基本構成単位であるα-アミノ酸を、選択的、かつ高感度に測定できる簡便・安価な新規分析法の開発』は、生体分析化学分野における喫緊の重要課題の一つである。本研究では、表面増強フォトクロミズムを用いたラベルフリーでのタンパク質、およびα-アミノ酸検出に関する定性・定量的な知見を得ることができた。
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Report
(4 results)
Research Products
(26 results)
