| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
We tried to construct the detection system of target cells with high sensitivity based on the signal amplification triggered by a protein highly expressed on the membrane of target cells. A nucleic acid aptamer with arbitrary tag sequence was used as an initiator of the amplification reaction. A single stranded DNA which can work as a trigger for DNA circuit of signal amplification reaction was successfully released from a duplex by the strand exchange with the tag tethered with aptamer bound on the target cell surface. Specific initiation of DNA circuit by the trigger was confirmed in vitro. Then, we tried to detect the target cell by the initiation of DNA circuit using the trigger specifically released by the tag on the target cell. However, signal amplification was not efficient. The optimization of condition of DNA circuit should be needed for obtaining enough signal to detect target cells.
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