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Development of sensitive biosensing methods by functional biochips with nano-scale layered structure

Research Project

Project/Area Number 16K05824
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Analytical chemistry
Research InstitutionTokyo University of Technology

Principal Investigator

YANO Kazuyoshi  東京工科大学, 応用生物学部, 教授 (40262109)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsプロテオミクス / アプタマー / プラズマ重合 / 分析化学 / 薄膜 / 蛍光増強 / バイオセンシング / 蛍光
Outline of Final Research Achievements

Nano-scale layered structure was fabricated on glass slides modified with silver layer as a metal mirror and with plasma-polymerized film as an optical interference layer. Acetonitrile was utilized as a monomer aiming for the purpose of creating amino group on the surface of plasma-polymerized film. It was shown that fluorescence intensity of Cy5-labeled target DNA was enhanced 6-fold on the modified glass slide compared with the unmodified one, resulting in highly sensitive detection. Amino group-modified probe DNA was immobilized on the surface of acetonitrile film via glutaraldehyde and successfully hybridized to Cy5-labeled target DNA, which was confirmed by enhanced fluorescence intensity. Furthermore, it was confirmed by fourier transform infrared spectroscopy (FT-IR) that amino group was present on the surface of plasma-polymerized film.

Academic Significance and Societal Importance of the Research Achievements

本研究は疾病の早期発見を目指し、従来半導体微細加工に利用されてきたプラズマ重合法をバイオテクノロジーの分野に活用しようとするものである。今回の科研費助成事業により、目的物質の検出シグナルを増強させることに成功できたため、本技術が医療分野に貢献できる可能性を示すことができた。またプラズマ重合法はドライプロセスによる一括加工が可能で大量生産に適した技術であるため、将来の事業化という観点からも極めて優れたポテンシャルを有しており、大変意義深い。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2018 2017

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results)

  • [Journal Article] Enhancement of Fluorescence-Based Sandwich Immunoassay Using Multilayered Microplates Modified with Plasma-Polymerized Films2017

    • Author(s)
      Kazuyoshi Yano and Akira Iwasaki
    • Journal Title

      Sensors

      Volume: 17 Issue: 1 Pages: 37-46

    • DOI

      10.3390/s17010037

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 蛍光増強のためのナノ積層基板の作製と固定化DNAによるバイオアッセイへの応用2018

    • Author(s)
      松家祐太郎、岡田麻衣子、杉本岩雄、矢野和義
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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