| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
So far, we have developed a technology for cutting out a predetermined DNA fragment from long DNA using peptide nucleic acid (PNA) and a technology for purifying a target DNA fragment from many DNA fragments. In this study, we focused on the development of a new method to analyze transcription factors those bind to specific sites of DNA by fusing our technologies. As a result, we succeeded in isolating and purifying DNA fragments prepared using PNA and an enzyme that specifically cleaves single-stranded DNA portion. In addition, it has become clear that the cerium (IV)-ethylenediaminetetraacetic acid system conventionally used as an artificial enzyme system is insufficient in DNA cleavage activity. The search for catalyst system has shown that cerium oxide nanoparticles are promising.
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