| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Here we developed a novel kinase assay system by using fluorescent liposome and membrane penetrating compounds. The newly prepared oligoarginines possessing a hydrophobic group and guanidinated polyallylamines showed effective translocation activities against the lipid membrane of the liposomes. The highly activated membrane penetrating compounds provided an effective kinase assay system, which needed a tiny amount of the enzyme reaction mixture, c.a. 0.56 microliter, to achieve the fluorescence assay.
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