| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this study, a selective extraction method for phospholipids with fluorous affinity has been developed. Perfluoroalkyl-containing compounds show unique “fluorous” properties, which are related to the specific affinity between perfluoroalkyl groups. Therefore, fluorous compounds can be easily and selectively extracted utilizing fluorous solvents, such as perfluoroalkanes. In this method, phospholipids could be selectively extracted into fluorous solvent with capturing by metal-immobilized perfluoropolyether carboxylic acid. Furthermore, this method was successfully applied to extraction of phospholipids in human plasma and they could be analyzed by LC-MS/MS without any interference. This result supported that this method will be a powerful tool for phospholipids analysis in targeted lipidomics.
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